Anti-MCL1 抗体 (ab28147)
Key features and details
- Rabbit polyclonal to MCL1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Rabbit, Guinea pig, Dog, Human, Pig, Monkey
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-MCL1 antibody
MCL1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MCL1 -
由来種
Rabbit -
特異性
This antibody detects an ~42/43 kDa protein doublet, corresponding to the expected molecular mass of Mcl1 on SDS-PAGE immunoblots. The antibody specificity is confirmed by peptide inhibition studies. An additional protein at ~33kDa may also be detected. Other additional proteins may also be seen in some samples. -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Rabbit, Guinea pig, Dog, Human, Pig, Monkey -
免疫原
Synthetic peptide corresponding to Human MCL1 aa 121-139 conjugated to Keyhole Limpet Haemocyanin (KLH).
Sequence:SPEEELDGYEPEPLGKRPA
-
ポジティブ・コントロール
- ICC/IF: Human HeLa cells; IHC-P: Human breast cancer tissue; WB: Hela, MDCK and RK-13 whole cell lysates.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab28147の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
|
|
WB |
Use a concentration of 0.1 µg/ml. Predicted molecular weight: 42-43 kDa.
0.1 µg/ml was sufficient for detection of Mcl1 in 20µg of HeLa Heat Shocked Cell Lysate by ECL immunoblot analysis. |
|
ICC/IF |
Use at an assay dependent concentration.
|
特記事項 |
---|
IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 0.1 µg/ml. Predicted molecular weight: 42-43 kDa. 0.1 µg/ml was sufficient for detection of Mcl1 in 20µg of HeLa Heat Shocked Cell Lysate by ECL immunoblot analysis. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
-
機能
Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis. -
配列類似性
Belongs to the Bcl-2 family. -
翻訳後修飾
Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.
Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.
Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites. -
細胞内局在
Membrane. Cytoplasm. Mitochondrion. Nucleus > nucleoplasm. Cytoplasmic, associated with mitochondria. - Information by UniProt
-
参照データベース
- Entrez Gene: 403537 Dog
- Entrez Gene: 4170 Human
- Entrez Gene: 100359222 Rabbit
- Omim: 159552 Human
- SwissProt: Q8HYS5 Dog
- SwissProt: Q07820 Human
- Unigene: 632486 Human
-
別名
- Bcl 2 related protein EAT/mcl1 antibody
- Bcl-2-like protein 3 antibody
- Bcl-2-related protein EAT/mcl1 antibody
see all
画像
-
All lanes : Anti-MCL1 antibody (ab28147) at 1/500 dilution
Lane 2 : HeLa whole cell lysate
Lane 3 : 3T3 whole cell lysate
Lane 4 : PC-12 whole cell lysate
Lane 5 : MDCK whole cell lysate
Lane 6 : RK-13 whole cell lysate
Predicted band size: 42-43 kDa -
ab28147 staining MCL1 in human breast cancer tissue cells by Immunohistochemistry
-
ab28147 staining MCL1 in human HeLa cells by Immunocytochemistry/ immunofluorescence.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (11)
ab28147 は 11 報の論文で使用されています。
- Tonozuka Y et al. The combination of brentuximab vedotin and chidamide synergistically suppresses the proliferation of T-cell lymphoma cells through the enhancement of apoptosis. Cancer Chemother Pharmacol 93:137-149 (2024). PubMed: 37921901
- Thus YJ et al. Inhibition of casein kinase 2 sensitizes mantle cell lymphoma to venetoclax through MCL-1 downregulation. Haematologica 108:797-810 (2023). PubMed: 36226498
- Zhu Y et al. miR-340-5p Mediates Cardiomyocyte Oxidative Stress in Diabetes-Induced Cardiac Dysfunction by Targeting Mcl-1. Oxid Med Cell Longev 2022:3182931 (2022). PubMed: 35126811
- Hoffmeister-Wittmann P et al. Bcl-xL as prognostic marker and potential therapeutic target in cholangiocarcinoma. Liver Int 42:2855-2870 (2022). PubMed: 35983950
- Yu B et al. Androgen receptor splicing variant 7 (ARV7) inhibits docetaxel sensitivity by inactivating the spindle assembly checkpoint. J Biol Chem 296:100276 (2021). PubMed: 33428943