Anti-mCherry 抗体

• IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-mCherry antibody (AB183628)

Whole mount immunohistochemical analysis of 2 days-post-fertilization zebrafish embryos labeling mCherry-expressing cells with ab183628 at 1/100 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody (AB183628)

Immunofluorescent analysis of mCherry fusion protein-transfected HeLa (Human epithelial cell line from cervix adenocarcinoma) cells. Cells were fixed with 4% paraformaldehyde for 30 minutes at room temperature and then permeabilized with 0.1% Triton X-100 for 1 minute. mCherry (green) was labeled using ab183628 at a dilution of 1/500.

Red : mCherry fusion protein is expressed in the transfected cell.
Blue : Hoechst 33342 staining.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody (AB183628)

Immunofluorescent analysis of mCherry fusion protein-transfected HeLa (Human epithelial cell line from cervix adenocarcinoma) cells. Cells were fixed with 4% paraformaldehyde for 30 minutes at room temperature and then permeabilized with 0.1% Triton X-100 for 1 minute. mCherry (green) was labeled using ab183628 at a dilution of 1/500.

Red : mCherry fusion protein is expressed in the transfected cell.
Blue : Hoechst 33342 staining.

• IP

Supplier Data

Immunoprecipitation - Anti-mCherry antibody (AB183628)

Immunoprecipitation analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate/extract using ab183628 at a 1/3000 dilution.

Lane 1 : 20 μg whole cell lysate/extract of Cherry protein-expressing HEK-293T cells.
Lane 2 : Control with 2.5 μg of pre-immune rabbit IgG.
Lane 3 : Immunoprecipitation of mCherry protein with 2.5 μg of ab183628.

7.5% SDS-PAGE. An anti-rabbit IgG (HRP) was used as a secondary reagent.

All lanes:

Immunoprecipitation - Anti-mCherry antibody (ab183628)

true

• ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody (AB183628)

RABV and mGluR2 are internalized into cells and transported together in early and late endosomes.

N2a (Mouse neuroblastoma cells) cells were stained by using the Tyramide Signal Amplification immunofluorescent method. N2a cells infected with ERA-N-mCherry for 20 minutes at 37°C were used to perform immunofluorescence staining for RABV antigen (red), mGluR2 (green), Rab5 or Rab7 (purple), and the cell nuclei (blue). Co-localization of the RABV-mGluR2 complex with Rab5 (E, F).

Wang et al PLoS Pathog. 2018 Jul 20;14(7):e1007189. doi: 10.1371/journal.ppat.1007189. eCollection 2018 Jul. Fig 7. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• WB

Supplier Data

Western blot - Anti-mCherry antibody (AB183628)

All lanes:

Western blot - Anti-mCherry antibody (ab183628) at 1/5000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate/extract at 30 µg

Lane 2:

Whole cell lysate/extract of mCherry-tagged protein expressing HEK-293T cells at 30 µg

Predicted band size: 27 kDa

false

• WB

Supplier Data

Western blot - Anti-mCherry antibody (AB183628)

All lanes:

Western blot - Anti-mCherry antibody (ab183628) at 1/10000 dilution

Lane 1:

Non-transfected 293T whole cell lysates at 30 µg

Lane 2:

GFP tagged protein-transfected 293T whole cell lysates at 30 µg

Lane 3:

RFP tagged protein-transfected 293T whole cell lysates at 30 µg

Lane 4:

mCherry tagged protein-transfected 293T whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 27 kDa

false

• WB

Supplier Data

Western blot - Anti-mCherry antibody (AB183628)

All lanes:

Western blot - Anti-mCherry antibody (ab183628) at 1/2000 dilution

Lane 1:

Full length recombinant mCherry at 0.005 µg

Lane 2:

Full length recombinant mCherry at 0.01 µg

Lane 3:

Full length recombinant mCherry at 0.02 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 27 kDa

false

• WB

Supplier Data

Western blot - Anti-mCherry antibody (AB183628)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-mCherry antibody (ab183628) at 1/5000 dilution

Lane 1:

Non-transfected 293T whole cell lysates at 30 µg

Lane 2:

PLK4-mCherry-transfected 293T whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 27 kDa

false