AB274415

Anti-MCH 抗体 [EPR23778-94]

Name: Anti-MCH 抗体 [EPR23778-94] (ab274415) | Abcam
Brand: Abcam Limited
SKU: AB274415
Price: 30000 JPY
Availability: InStock
Rating: 5 (4 reviews)

Anti-MCH antibody [EPR23778-94]

BOND RX™ Validated
RabMAb
Recombinant
20ul selling size
詳細を見る

(4 Reviews)

(3 Publications)

Rabbit Recombinant Monoclonal MCH antibody. Suitable for IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

別名を表示する

MCH, PMCH, Pro-MCH

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (AB274415)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (AB274415)

Immunohistochemical analysis of paraffin-embedded Human hypothalamus tissue labeling PMCH with ab274415 at 1/2000 (0.271 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human hypothalamus (PMID : 30420052). The section was incubated with ab274415 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (AB274415)

Immunohistochemical analysis of paraffin-embedded Rat hypothalamus tissue labeling PMCH with ab274415 at 1/2000 (0.271 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat hypothalamus (PMID : 30420052). The section was incubated with ab274415 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Frozen sections) - Anti-MCH antibody [EPR23778-94] (AB274415)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-MCH antibody [EPR23778-94] (AB274415)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hypothalamus tissue labeling PMCH with ab274415 at 1/500 (1.084 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on mouse hypothalamus is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-MCH antibody [EPR23778-94] (AB274415)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hypothalamus tissue labeling PMCH with ab274415 at 1/500 (1.084 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on rat hypothalamus is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (AB274415)

Immunohistochemical analysis of paraffin-embedded Mouse hypothalamus tissue labeling PMCH with ab274415 at 1/2000 (0.271 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse hypothalamus (PMID : 30420052). The section was incubated with ab274415 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR23778-94

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

IHC-Fr, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

This antibody recognizes the PMCH chain.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Common marmoset": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Melanin-concentrating hormone (MCH) originally isolated from fish is a peptide hormone that plays a significant role in energy balance and feeding behavior. It is about 19 amino acids long in humans and has a molecular mass of approximately 2.4 kDa. MCH is mainly expressed in the lateral hypothalamus and zona incerta of the brain which suggests its central role in neurologic function. This peptide binds to MCH receptor 1 (MCHR1) influencing various signaling pathways.

Biological function summary

MCH affects a range of physiological processes and is part of the neuropeptide signaling system. It regulates feeding and energy homeostasis by influencing appetite increase and reducing energy expenditure. MCH operates within a complex network of neurotransmitters and hormones in the central nervous system. Its interactions with other neuropeptides such as neuropeptide Y (NPY) and agouti-related peptide (AgRP) support its role in the complex regulation of energy homeostasis.

Pathways

MCH engages in signaling linked to G-protein coupled receptors (GPCRs) connecting with pathways that regulate food intake and energy management. It influences the orexin/hypocretin pathway which also modulates sleep-wake cycles and arousal states. The interaction with orexin highlights MCH's broad regulatory influence balancing energy levels and adaptive responses. The protein's signaling is associated with molecules like leptin and insulin marking its integration into wider metabolic pathways.

MCH is linked to obesity and mood disorders due to its influence on appetite and emotional regulation. A dysregulated MCH signaling pathway is associated with overeating and weight gain highlighting its role in obesity physiology. Additionally altered levels of MCH could contribute to mood disorders such as depression where its interaction with serotonin and dopamine pathways may impact emotional stability.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

MCH may act as a neurotransmitter or neuromodulator in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. May also have a role in spermatocyte differentiation.

See full target information PMCH

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in nutrition 11:1468874 PubMed39758319

2025

Melanin-concentrating hormone attenuates the hedonic feeding induced by orexin-A in the ventral tegmental area of high-fat diet male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoning Liu,Helin Yang,Wenguang Xu,Xuezhe Wang,Wenhui Tang,Xiaoxuan Wang,Yang Jiao,Xinchi Luan,Pengmeng Li,Feifei Guo

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 49:1468-1480 PubMed38734818

2024

Ventral subiculum promotes wakefulness through several pathways in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xue-Fen Zhang,Yi-Dan Li,Yue Li,Ying Li,Dan Xu,Lin-Lin Bi,Hai-Bo Xu

Neuroendocrinology 114:605-622 PubMed38547853

2024

Regulation of the MCHergic Neural Circuit to Dorsal Raphe Nucleus on Emotion-Related Behaviors and Intestinal Dysfunction in Mice Model of Irritable Bowel Syndrome with Diarrhea.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Ming,Shengli Gao,Jinqiu Sun,Nana Zhang,Ruixiao Guo,Xufei Feng,Xinchi Luan,Han Xing,Yang Jiao,Feifei Guo

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MCH 抗体 [EPR23778-94]

Anti-MCH antibody [EPR23778-94]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (AB274415)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (AB274415)

Immunohistochemistry (Frozen sections) - Anti-MCH antibody [EPR23778-94] (AB274415)

Immunohistochemistry (Frozen sections) - Anti-MCH antibody [EPR23778-94] (AB274415)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (AB274415)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

Abcam product promise