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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-MCAK 抗体 [EPR14838] (ab187652)

Submit a review Submit a question References (4)
Western blot - Anti-MCAK antibody [EPR14838] (ab187652)
  • Flow Cytometry (Intracellular) - Anti-MCAK antibody [EPR14838] (ab187652)
  • Western blot - Anti-MCAK antibody [EPR14838] (ab187652)
  • Immunocytochemistry/ Immunofluorescence - Anti-MCAK antibody [EPR14838] (ab187652)
  • Immunocytochemistry/ Immunofluorescence - Anti-MCAK antibody [EPR14838] (ab187652)
  • Anti-MCAK antibody [EPR14838] (ab187652)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14838] to MCAK
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free PE

製品の概要

  • 製品名

    Anti-MCAK antibody [EPR14838]
    MCAK 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14838] to MCAK

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), ICC/IF, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • MCF7, 293T, Hela and K562 cell lysates. Hela and MCF7 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR14838

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab187652の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/260.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/100.
WB
1/1000 - 1/2000. Predicted molecular weight: 81 kDa.
特記事項
Flow Cyt (Intra)
1/260.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/100.
WB
1/1000 - 1/2000. Predicted molecular weight: 81 kDa.

ターゲット情報

  • 機能

    Promotes ATP-dependent removal of tubulin dimers from microtubules. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis.

  • 組織特異性

    Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific.

  • 配列類似性

    Belongs to the kinesin-like protein family. MCAK/KIF2 subfamily.
    Contains 1 kinesin-motor domain.

  • 発生段階

    Isoform 2 is expressed in fetal testis.

  • ドメイン

    The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.

  • 翻訳後修飾

    Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by STK12, regulates association with centromeres and kinetochores and the microtubule depolymerization activity.

  • 細胞内局在

    Cytoplasm > cytoskeleton. Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Centromeric localization requires the presence of BUB1 and SGOL2.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 4930402F02Rik antibody
      • CT139 antibody
      • ESTM5 antibody
      • KIF 2C antibody
      • kif2c antibody
      • KIF2C_HUMAN antibody
      • Kinesein Family Member 2C antibody
      • Kinesin family member 2C antibody
      • kinesin like 6 (mitotic centromere associated kinesin) antibody
      • Kinesin like 6 antibody
      • Kinesin like protein 6 antibody
      • Kinesin like protein KIF2C antibody
      • Kinesin-like protein 6 antibody
      • Kinesin-like protein KIF2C antibody
      • KNS L6 antibody
      • KNSL 6 antibody
      • KNSL6 antibody
      • MCAK antibody
      • MGC11883 antibody
      • Mitotic centromere associated kinesin antibody
      • Mitotic centromere-associated kinesin antibody
      • OTTHUMP00000010066 antibody
      • X83316 antibody
      see all

    画像

    • Western blot - Anti-MCAK antibody [EPR14838] (ab187652)
      Western blot - Anti-MCAK antibody [EPR14838] (ab187652)

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: MCAK knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: HEK293 whole cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab187652 observed at 90 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab187652 was shown to specifically react with MCAK when MCAK knockout samples were used. Wild-type and MCAK knockout samples were subjected to SDS-PAGE.  Ab187652 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

       

    • Flow Cytometry (Intracellular) - Anti-MCAK antibody [EPR14838] (ab187652)
      Flow Cytometry (Intracellular) - Anti-MCAK antibody [EPR14838] (ab187652)

      Intracellular flow cytometric analysis of HeLa cells labeling MCAK with ab187652 at 1/260 dilution compared to negative control cells. Secondary antibody FITC-conjugated goat-anti-rabbit IgG used at 1/150 diution. 

       

       

    • Western blot - Anti-MCAK antibody [EPR14838] (ab187652)
      Western blot - Anti-MCAK antibody [EPR14838] (ab187652)
      All lanes : Anti-MCAK antibody [EPR14838] (ab187652) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
      Lane 3 : Hela cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 81 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-MCAK antibody [EPR14838] (ab187652)
      Immunocytochemistry/ Immunofluorescence - Anti-MCAK antibody [EPR14838] (ab187652)

      Immunofluorscence analysis of MCF7 cells labeling MCAK with ab187652 at 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter-stained with Dapi.

    • Immunocytochemistry/ Immunofluorescence - Anti-MCAK antibody [EPR14838] (ab187652)
      Immunocytochemistry/ Immunofluorescence - Anti-MCAK antibody [EPR14838] (ab187652)

      Immunofluorscence analysis of HeLa cells labeling MCAK with ab187652 at 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter-stained with Dapi.

    • Anti-MCAK antibody [EPR14838] (ab187652)
      Anti-MCAK antibody [EPR14838] (ab187652)

    参考文献 (4)

    ab187652 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab187652 は 4 報の論文で使用されています。

    • Li Q  et al. CKAP2L, a crucial target of miR-326, promotes prostate cancer progression. BMC Cancer 22:666 (2022). PubMed: 35715760
    • Marquis C  et al. Chromosomally unstable tumor cells specifically require KIF18A for proliferation. Nat Commun 12:1213 (2021). PubMed: 33619254
    • Hadders MA  et al. Untangling the contribution of Haspin and Bub1 to Aurora B function during mitosis. J Cell Biol 219:N/A (2020). PubMed: 32027339
    • Sellerio AL  et al. Overshoot during phenotypic switching of cancer cell populations. Sci Rep 5:15464 (2015). PubMed: 26494317

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