Anti-MCAK 抗体 (ab70536)
Key features and details
- Rabbit polyclonal to MCAK
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MCAK antibody
MCAK 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MCAK -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, IPmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human MCAK.
Database link: Q99661 -
ポジティブ･コントロール
- IHC: human skin carcinoma and breast carcinoma tissues IP: HeLa cell lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70536の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IP |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Promotes ATP-dependent removal of tubulin dimers from microtubules. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis. -
組織特異性
Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific. -
配列類似性
Belongs to the kinesin-like protein family. MCAK/KIF2 subfamily.
Contains 1 kinesin-motor domain. -
発生段階
Isoform 2 is expressed in fetal testis. -
ドメイン
The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by STK12, regulates association with centromeres and kinetochores and the microtubule depolymerization activity. -
細胞内局在
Cytoplasm > cytoskeleton. Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Centromeric localization requires the presence of BUB1 and SGOL2. - Information by UniProt
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参照データベース
- Entrez Gene: 11004 Human
- Omim: 604538 Human
- SwissProt: Q99661 Human
- Unigene: 720061 Human
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別名
- 4930402F02Rik antibody
- CT139 antibody
- ESTM5 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCAK antibody (ab70536)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma (left) and breast carcinoma (right) tissues labelling MCAK with ab70536 at 1/1000 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-MCAK antibody (ab70536)MCAK was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab70536, ab264148 and ab264149 at 3 µg per reaction. Western blot was performed from the immunoprecipitate using ab264148 at 1 µg/ml.
Lane 1: ab70536 IP in HeLa whole cell lysate.
Lane 2: ab264148 IP in HeLa whole cell lysate.
Lane 3: ab264149 IP in HeLa whole cell lysate.
Lane 4: Control IgG.Exposure time: 10 seconds.
Detection: Chemiluminescence
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab70536 は論文での使用が確認できていません。