Anti-MCAK 抗体 [2488C3a] (ab50778)
![Western blot - Anti-MCAK antibody [2488C3a] (ab50778)](https://www.abcam.co.jp/ps/products/50/ab50778/Images/ab50778-270786-ab50778KOWB.jpg "Western blot - Anti-MCAK antibody [2488C3a] (ab50778)")
Key features and details
- Mouse monoclonal [2488C3a] to MCAK
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-MCAK antibody [2488C3a]
MCAK 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2488C3a] to MCAK -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant human MCAK fragment
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. -
ポリ/モノ
モノクローナル -
クローン名
2488C3a -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50778の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 81 kDa.
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| 特記事項 |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 81 kDa. |
ターゲット情報
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機能
Promotes ATP-dependent removal of tubulin dimers from microtubules. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis. -
組織特異性
Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific. -
配列類似性
Belongs to the kinesin-like protein family. MCAK/KIF2 subfamily.
Contains 1 kinesin-motor domain. -
発生段階
Isoform 2 is expressed in fetal testis. -
ドメイン
The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by STK12, regulates association with centromeres and kinetochores and the microtubule depolymerization activity. -
細胞内局在
Cytoplasm > cytoskeleton. Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Centromeric localization requires the presence of BUB1 and SGOL2. - Information by UniProt
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参照データベース
- Entrez Gene: 11004 Human
- Omim: 604538 Human
- SwissProt: Q99661 Human
- Unigene: 720061 Human
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別名
- 4930402F02Rik antibody
- CT139 antibody
- ESTM5 antibody
see all
画像
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Western blot - Anti-MCAK antibody [2488C3a] (ab50778)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: MCAK knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HEK293 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab50778 observed at 90 kDa. Red - loading control, ab176560, observed at 50 kDa.ab50778 was shown to specifically react with MCAK when MCAK knockout samples were used. Wild-type and MCAK knockout samples were subjected to SDS-PAGE. Ab50778 and ab176560 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-MCAK antibody [2488C3a] (ab50778)](https://www.abcam.co.jp/ps/products/50/ab50778/Images/BMR00446.jpg)
Western blot - Anti-MCAK antibody [2488C3a] (ab50778)Anti-MCAK antibody [2488C3a] (ab50778) + immunizing peptide
Predicted band size: 81 kDa
データシートおよび資料
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Datasheet download
参考文献 (2)
ab50778 は 2 報の論文で使用されています。
- D'Angelo L et al. MCAK-mediated regulation of endothelial cell microtubule dynamics is mechanosensitive to myosin-II contractility. Mol Biol Cell 28:1223-1237 (2017). PubMed: 28298485
- Braun A et al. Rac1 and Aurora A regulate MCAK to polarize microtubule growth in migrating endothelial cells. J Cell Biol 206:97-112 (2014). PubMed: 25002679
