Anti-MCAF1 抗体 (ab84497)
Key features and details
- Rabbit polyclonal to MCAF1
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-MCAF1 antibody -
製品の詳細
Rabbit polyclonal to MCAF1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
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免疫原
Synthetic peptide within Human MCAF1 aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. AAQ92978.1
Database link: Q6VMQ6 -
ポジティブ･コントロール
- Human small cell lung cancer. Mouse squamous cell carcinoma.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab84497の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/100 - 1/500.
Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. |
| 特記事項 |
|---|
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IHC-P
1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. |
ターゲット情報
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関連性
MCAF1 is a recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. It can act both as an activator or a repressor depending on the context. It mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. MCAF1 is required to stimulate histone methyltransferase activity of SETDB1 and facilitate the conversion of dimethylated to trimethylated H3 'Lys-9'. The complex formed with MBD1 and SETDB1 represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 55729 Human
- Entrez Gene: 54343 Mouse
- SwissProt: Q6VMQ6 Human
- SwissProt: Q7TT18 Mouse
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別名
- Activating transcription factor 7 interacting protein 1 antibody
- ATF interacting protein antibody
- ATF IP antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCAF1 antibody (ab84497)ab84497, at 1/250 dilution, staining MCAF1 in formalin-fixed, paraffin-embedded human small cell lung cancer by Immunohistochemistry, using DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCAF1 antibody (ab84497)ab84497, at 1/250 dilution, staining MCAF1 in formalin-fixed, paraffin-embedded mouse squamous cell carcinoma by Immunohistochemistry, using DAB staining.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab84497 は 7 報の論文で使用されています。
- Patel LR et al. Genome-wide CRISPR-Cas9 screen analyzed by SLIDER identifies network of repressor complexes that regulate TRIM24. iScience 26:107126 (2023). PubMed: 37426340
- Sin JH et al. ATF7ip Targets Transposable Elements for H3K9me3 Deposition to Modify CD8+ T Cell Effector and Memory Responses. J Immunol 208:1155-1169 (2022). PubMed: 35110421
- Tsusaka T et al. The fibronectin type-III (FNIII) domain of ATF7IP contributes to efficient transcriptional silencing mediated by the SETDB1 complex. Epigenetics Chromatin 13:52 (2020). PubMed: 33256805
- Sin JH et al. The epigenetic regulator ATF7ip inhibits Il2 expression, regulating Th17 responses. J Exp Med 216:2024-2037 (2019). PubMed: 31217192
- Tsusaka T et al. ATF7IP regulates SETDB1 nuclear localization and increases its ubiquitination. EMBO Rep 20:e48297 (2019). PubMed: 31576654
- Tsusaka T et al. Tri-methylation of ATF7IP by G9a/GLP recruits the chromodomain protein MPP8. Epigenetics Chromatin 11:56 (2018). PubMed: 30286792
- Minkovsky A et al. The Mbd1-Atf7ip-Setdb1 pathway contributes to the maintenance of X chromosome inactivation. Epigenetics Chromatin 7:12 (2014). Mouse . PubMed: 25028596