Anti-MC2-R 抗体 (ab77347)
Key features and details
- Goat polyclonal to MC2-R
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MC2-R antibody
MC2-R 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to MC2-R -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Sheep, Horse, Cow, Dog, Chimpanzee
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免疫原
Synthetic peptide corresponding to Human MC2-R aa 9-21 (N terminal). (NP_000520.1)
Sequence:ENINNTARNNSDC
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ポジティブ･コントロール
- Human skin lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab77347 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77347の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
| 特記事項 |
|---|
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Receptor for ACTH. This receptor is mediated by G proteins (G(s)) which activate adenylate cyclase. -
組織特異性
Melanocytes and corticoadrenal tissue. -
関連疾患
Defects in MC2R are the cause of glucocorticoid deficiency type 1 (GCCD1) [MIM:202200]; also known as familial glucocorticoid deficiency type 1 (FGD1). GCCD1 is an autosomal recessive disorder due to congenital insensitivity or resistance to adrenocorticotropin (ACTH). It is characterized by progressive primary adrenal insufficiency, without mineralocorticoid deficiency. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4158 Human
- Omim: 607397 Human
- SwissProt: P34974 Cow
- SwissProt: Q01718 Human
- SwissProt: Q9TU77 Sheep
- Unigene: 248144 Human
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別名
- ACTH receptor antibody
- ACTH-R antibody
- ACTHR antibody
see all
画像
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Western blot - Anti-MC2-R antibody (ab77347)Anti-MC2-R antibody (ab77347) at 1 µg/ml + Human skin lysate in RIPA buffer at 35 µg
Predicted band size: 34 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab77347 は 2 報の論文で使用されています。
- Wierzbicka JM et al. Differentiation of Keratinocytes Modulates Skin HPA Analog. J Cell Physiol 232:154-66 (2017). PubMed: 27061711
- Wierzbicka JM et al. Bioactive forms of vitamin D selectively stimulate the skin analog of the hypothalamus-pituitary-adrenal axis in human epidermal keratinocytes. Mol Cell Endocrinol 437:312-322 (2016). PubMed: 27524410