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リコンビナントRabMAb

Anti-MBIP 抗体 [EPR13951] (ab186750)

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Western blot - Anti-MBIP antibody [EPR13951] (ab186750)
  • Western blot - Anti-MBIP antibody [EPR13951] (ab186750)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBIP antibody [EPR13951] (ab186750)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBIP antibody [EPR13951] (ab186750)
  • Immunocytochemistry/ Immunofluorescence - Anti-MBIP antibody [EPR13951] (ab186750)
  • Flow Cytometry (Intracellular) - Anti-MBIP antibody [EPR13951] (ab186750)
  • Anti-MBIP antibody [EPR13951] (ab186750)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13951] to MBIP
  • Suitable for: WB, Flow Cyt (Intra), IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-MBIP antibody [EPR13951]
    MBIP 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13951] to MBIP

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, Flow Cyt (Intra), IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Hela and 293 cell lysates, Human fetal lung and Human fetal heart lysates; Human cardiac muscle and Human clear cell carcinoma of kidney tissues; HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR13951

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab186750の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
Flow Cyt (Intra)
1/400.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/500.
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
Flow Cyt (Intra)
1/400.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/500.

ターゲット情報

  • 機能

    Inhibits the MAP3K12 activity to induce the activation of the JNK/SAPK pathway. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4.

  • 組織特異性

    Ubiquitous. High expression seen in the heart and lung.

  • 細胞内局在

    Nucleus. Cytoplasm. Shows a cytoplasmic localization when coexpressed with MAP3K12.
  • Information by UniProt

  • 参照データベース

    • 別名

      • MAP3K12 binding inhibitory protein 1 antibody
      • MAP3K12-binding inhibitory protein 1 antibody
      • MAPK upstream kinase binding inhibitory protein antibody
      • MAPK upstream kinase-binding inhibitory protein antibody
      • Mbip antibody
      • MBIP1_HUMAN antibody
      • MUK binding inhibitory protein antibody
      • MUK-binding inhibitory protein antibody
      • OTTHUMP00000178844 antibody
      see all

    画像

    • Western blot - Anti-MBIP antibody [EPR13951] (ab186750)
      Western blot - Anti-MBIP antibody [EPR13951] (ab186750)
      All lanes : Anti-MBIP antibody [EPR13951] (ab186750) at 1/10000 dilution

      Lane 1 : Hela cell lysate
      Lane 2 : 293 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 39 kDa



      Blocking buffer:  5% NFDM/TBST

    • Western blot - Anti-MBIP antibody [EPR13951] (ab186750)
      Western blot - Anti-MBIP antibody [EPR13951] (ab186750)
      All lanes : Anti-MBIP antibody [EPR13951] (ab186750) at 1/10000 dilution

      Lane 1 : Human fetal lung tissue lysate
      Lane 2 : Human fetal heart tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 39 kDa



      Blocking buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBIP antibody [EPR13951] (ab186750)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBIP antibody [EPR13951] (ab186750)

      Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling MBIP with ab186750 at 1/250 dilution.  Secondary antibody: Ready to use HRP Polymer for Rabbit IgG. Counter stain: Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBIP antibody [EPR13951] (ab186750)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBIP antibody [EPR13951] (ab186750)

      Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma of kidney tissue labeling MBIP with ab186750 at 1/250 dilution. Secondary ab: ready to use HRP Polymer for Rabbit IgG. Counter stain: Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-MBIP antibody [EPR13951] (ab186750)
      Immunocytochemistry/ Immunofluorescence - Anti-MBIP antibody [EPR13951] (ab186750)

      Immunofluorescence analysis of HeLa cells labeling MBIP with ab186750 at 1/500 dilution.  Secondary antibody: Goat anti rabbit IgG (Alexa Fluor® 488).  Fixative: 4% paraformaldehyde. Counter stain: Dapi (blue).

    • Flow Cytometry (Intracellular) - Anti-MBIP antibody [EPR13951] (ab186750)
      Flow Cytometry (Intracellular) - Anti-MBIP antibody [EPR13951] (ab186750)

      Intracellular flow cytometric analysis of HeLa cell labeling MBIP usingab186750 at 1/400 dilution. Secondary antibody: Goat anti rabbit IgG (FITC) at 1/400 dilution. Fixative: 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG. 

       

       

    • Anti-MBIP antibody [EPR13951] (ab186750)
      Anti-MBIP antibody [EPR13951] (ab186750)

    参考文献 (0)

    ab186750 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab186750 は論文での使用が確認できていません。

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