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Anti-MBD2 + MBD3 抗体 [106B691] (ab45027)

Reviews (1)Q&A (1)References (11)
Western blot - Anti-MBD2 + MBD3 antibody [106B691] (ab45027)
  • Flow Cytometry (Intracellular) - Anti-MBD2 + MBD3 antibody [106B691] (ab45027)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 + MBD3 antibody [106B691] (ab45027)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 + MBD3 antibody [106B691] (ab45027)

Key features and details

  • Mouse monoclonal [106B691] to MBD2 + MBD3
  • Suitable for: IHC-P, Flow Cyt (Intra), ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-MBD2 + MBD3 antibody [106B691]

  • 製品の詳細

    Mouse monoclonal [106B691] to MBD2 + MBD3

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-P, Flow Cyt (Intra), ICC/IF, WBmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Xenopus laevis, Zebrafish

  • 免疫原

    Synthetic peptide corresponding to Human MBD2 + MBD3 aa 200-300. Synthetic peptide:

    CKAFMVTDEDIRKQEE

    , corresponding to amino acids 215-230 of Human MBD3
    Database link: Q9UBB5
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • HeLa cells (nuclear fraction). IHC-P: Human placenta tissue Flow Cyt (Intra): HeLa cells.

  • 特記事項

    The theoretical molecular weight of human MBD2 (411 amino acids) is 45 kD and human MBD3 (291 amino acids) is 33 kD.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein G purified

  • 一次抗体 備考

    The theoretical molecular weight of human MBD2 (411 amino acids) is 45 kD and human MBD3 (291 amino acids) is 33 kD.

  • ポリ/モノ

    モノクローナル

  • クローン名

    106B691

  • アイソタイプ

    IgG1

  • 軽鎖の種類

    kappa

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab45027の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/300.
Flow Cyt (Intra)
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF
Use at an assay dependent concentration.
WB (1)
Use a concentration of 2 µg/ml. Predicted molecular weight: 33, 45 kDa.
特記事項
IHC-P
1/300.
Flow Cyt (Intra)
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF
Use at an assay dependent concentration.
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 33, 45 kDa.

ターゲット情報

  • 関連性

    DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.

  • 細胞内局在

    Nuclear

  • 参照データベース

    see all

  • 別名

    • Demethylase antibody
    • DMTase antibody
    • MBD 2 antibody
    • MBD 3 antibody
    • Methyl CpG binding domain protein 2 antibody
    • Methyl CpG binding domain protein 3 antibody
    • Methyl CpG binding protein MBD2 antibody
    • Methyl CpG binding protein MBD3 antibody
    see all

画像

  • Western blot - Anti-MBD2 + MBD3 antibody [106B691] (ab45027)
    Western blot - Anti-MBD2 + MBD3 antibody [106B691] (ab45027)
    All lanes : Anti-MBD2 + MBD3 antibody [106B691] (ab45027) at 2 µg/ml

    Lane 1 : HeLa cell lysate (nuclear fraction) without immunising peptide
    Lane 2 : HeLa cell lysate (nuclear fraction) with immunising peptide

    Predicted band size: 33, 45 kDa
    Observed band size: 33,45 kDa why is the actual band size different from the predicted?
    Additional bands at: 160 kDa, 40 kDa. We are unsure as to the identity of these extra bands.

  • Flow Cytometry (Intracellular) - Anti-MBD2 + MBD3 antibody [106B691] (ab45027)
    Flow Cytometry (Intracellular) - Anti-MBD2 + MBD3 antibody [106B691] (ab45027)
    Overlay histogram showing HeLa cells stained with ab45027 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45027, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 + MBD3 antibody [106B691] (ab45027)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 + MBD3 antibody [106B691] (ab45027)Image courtesy of an anonymous Abreview.
    ab45027 at 2µg/ml staining MBD2 + MBD3 in human glioma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed and samples were blocked using 10% BSA. The secondary used was an HRP conjugated anti rabbit/mouse mix.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 + MBD3 antibody [106B691] (ab45027)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD2 + MBD3 antibody [106B691] (ab45027)
     
    Immunohistochemistry analysis of immersion fixed paraffin-embedded sections of human placenta labeling MBD2 + MBD3 with  ab45027 at 1/300 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).

     

参考文献 (11)

ab45027 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab45027 は 11 報の論文で使用されています。

  • Liao R  et al. BCL11B and the NuRD complex cooperatively guard T-cell fate and inhibit OPA1-mediated mitochondrial fusion in T cells. EMBO J 42:e113448 (2023). PubMed: 37737560
  • Kraushaar DC  et al. The gene repressor complex NuRD interacts with the histone variant H3.3 at promoters of active genes. Genome Res 28:1646-1655 (2018). PubMed: 30254051
  • Xu M  et al. MeCP2 suppresses LIN28A expression via binding to its methylated-CpG islands in pancreatic cancer cells. Oncotarget 7:14476-85 (2016). WB . PubMed: 26910839
  • Gómez-Del Arco P  et al. The Chromatin Remodeling Complex Chd4/NuRD Controls Striated Muscle Identity and Metabolic Homeostasis. Cell Metab 23:881-92 (2016). PubMed: 27166947
  • Teperek M  et al. Sperm and spermatids contain different proteins and bind distinct egg factors. Int J Mol Sci 15:16719-40 (2014). WB ; Frog . PubMed: 25244019
View all Publications for this product

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-MBD2 + MBD3 antibody [106B691] - ChIP Grade

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (glioma cell line)
Loading amount
20 µg
Specification
glioma cell line
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 13 2010

Question

Interested in using ab45027 with rat samples.

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Answer

Thanks for your call today and for your questions about ab45027. Please see below for information about our testing discount program, and let me know if you have any questions. Since ab45027 has not been tested in rat, I can offer a discount off a future purchase if you buy ab45027 now, test it inrat samples,and submit feedback to us in the form of an Abreview.The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab45027 in rat. I will then send a discount code. This code must be issued before purchasing ab45027 so please wait for my reply before ordering. 2. Purchase ab45027 either by phone, fax, or online (www.abcam.com). 3. Test it in rat. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab45027 turns out to be unsuitable for rat, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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