AB9700

Anti-MAX 抗体

Name: Anti-MAX 抗体 (ab9700) | Abcam
Brand: Abcam Limited
SKU: AB9700
Price: 74000 JPY
Availability: InStock

Anti-MAX antibody

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(2 Publications)

Goat Polyclonal MAX antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human MAX aa 100 to C-terminus.

別名を表示する

BHLHD4, MAX, Protein max, Class D basic helix-loop-helix protein 4, Myc-associated factor X, bHLHd4

1 Images

Supplier Data

Western blot - Anti-MAX antibody (AB9700)

Primary incubation for 1 hour. Detected using chemiluminescence.

All lanes:

Western blot - Anti-MAX antibody (ab9700) at 0.01 µg/mL

All lanes:

Jurkat cell lysate in RIPA buffer at 35 µg

Predicted band size: 18 kDa

Observed band size: 22 kDa

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Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P, WB

applications

免疫原

Synthetic Peptide within Human MAX aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P61244

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

バッファー組成

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The MAX protein also known as Myc-associated factor X functions mechanically as a transcription factor with a molecular weight of approximately 20 kDa. MAX is expressed in a wide range of tissues throughout the body. The protein forms homodimers or heterodimers with other members of the basic-helix-loop-helix-zipper (bHLHZ) family such as MYC MAD and MNT. The dimerization with different partners determines its role as an activator or repressor of transcription affecting the regulation of various genes.

Biological function summary

The MAX protein plays a significant role in controlling cell growth differentiation and apoptosis. The protein often forms part of complex regulatory networks within the cell. By interacting with different partner proteins MAX modulates transcriptional activity affecting cellular outcomes. Importantly it can switch between activating or repressing target genes depending on the dimer partner influencing processes such as proliferation and differentiation.

Pathways

MAX is deeply integrated into the MYC-MAX-MAD pathway an important pathway for regulating gene expression linked to cell cycle and proliferative processes. Within this pathway MAX pairs with either MYC or MAD depending on the cellular context to influence transcription. When paired with MYC it promotes transcription while pairing with MAD results in transcriptional repression. Additionally in the growth signaling pathway the MAX protein’s regulation of target genes is pivotal for ensuring proper cellular responses to growth signals.

The malfunction of the MAX protein has a strong association with cancer and neurodegenerative diseases. Alterations in MAX function can lead to uncontrolled cell growth contributing to oncogenesis particularly in relation to MYC which is a known oncogene. Furthermore dysregulation of the MAX protein is linked to neurodegenerative disorders where abnormal MAX activity may disrupt normal neural gene expression enhancing disease progression.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC : MAX complex is a transcriptional activator, whereas the MAD : MAX complex is a repressor. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. Represses MYC transcriptional activity from E-box elements.

See full target information MAX

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 10:537763 PubMed33251130

2020

Long Non-Coding RNA MEG3 Modifies Cell-Cycle, Migration, Invasion, and Proliferation Through AKAP12 by Sponging miR-29c in Meningioma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chenyu Ding,Xuehan Yi,Jiaheng Xu,Zhenhua Huang,Xingyao Bu,Desheng Wang,Hongliang Ge,Gaoqi Zhang,Jianjun Gu,Dezhi Kang,Xiyue Wu

Cell chemical biology 26:711-723.e14 PubMed30880155

2019

Stabilization of the Max Homodimer with a Small Molecule Attenuates Myc-Driven Transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas B Struntz,Andrew Chen,Anja Deutzmann,Robert M Wilson,Eric Stefan,Helen L Evans,Maricela A Ramirez,Tong Liang,Francisco Caballero,Mattheus H E Wildschut,Dylan V Neel,David B Freeman,Marius S Pop,Marie McConkey,Sandrine Muller,Brice H Curtin,Hanna Tseng,Kristen R Frombach,Vincent L Butty,Stuart S Levine,Clementine Feau,Sarah Elmiligy,Jiyoung A Hong,Timothy A Lewis,Amedeo Vetere,Paul A Clemons,Scott E Malstrom,Benjamin L Ebert,Charles Y Lin,Dean W Felsher,Angela N Koehler

View all publications

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