Anti-Matrin 3 抗体 (ab84422)
Key features and details
- Rabbit polyclonal to Matrin 3
- Suitable for: IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Matrin 3 antibody
Matrin 3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Matrin 3 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Chinese hamster, Orangutan, Bat
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免疫原
Synthetic peptide designed within residues: GFYCKLCSLF YTNEEVAKNT HCSSLPHYQK LKKFLNKLAE ERRQKKET, corresponding to C terminal amino acids 800-847 of Human Matrin 3 (NP_061322.2)
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ポジティブ･コントロール
- IHC: Human: breast carcinoma, colon adenocarcinoma. Mouse: squamous cell carcinoma.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab84422の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Note: expected to work on frozen sections.
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| ICC/IF | (2) |
1/100.
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| 特記事項 |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Note: expected to work on frozen sections. |
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ICC/IF
1/100. |
ターゲット情報
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機能
May play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. In association with the SFPQ-NONO heteromer may play a role in nuclear retention of defective RNAs. -
関連疾患
Defects in MATR3 are the cause of myopathy distal type 2 (MPD2) [MIM:606070]; also called vocal cord and pharyngeal dysfunction with distal myopathy (VCPDM). MPD2 is a muscular disorder characterized by distal weakness, with onset in hands and feet, associated with vocal cord and pharyngeal weakness causing a nasal voice and swallowing disorders. -
配列類似性
Contains 1 matrin-type zinc finger.
Contains 2 RRM (RNA recognition motif) domains. -
細胞内局在
Nucleus matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 9782 Human
- Entrez Gene: 17184 Mouse
- Omim: 164015 Human
- SwissProt: P43243 Human
- SwissProt: Q8K310 Mouse
- Unigene: 268939 Human
- Unigene: 595110 Human
- Unigene: 215034 Mouse
see all -
別名
- ALS21 antibody
- KIAA0723 antibody
- Matr3 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody (ab84422)Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human colon adenocarcinoma using 1/250 ab84422, followed by DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody (ab84422)Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Mouse squamous cell carcinoma using 1/250 ab84422, followed by DAB staining. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody (ab84422)Detection of Human Matrin 3 by Immunofluorescence.
Sample: FFPE section of human breast carcinoma.Antibody: ab84422 used at a dilution of 1/100.
Detection: Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG used at a dilution of 1/500. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody (ab84422)Detection of Human Matrin 3 by Immunofluorescence.
Sample: FFPE section of Human breast carcinoma.Antibody: ab84422 used at a dilution of 1/100.
Detection: Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG used at a dilution of 1/500. -
Immunocytochemistry/ Immunofluorescence - Anti-Matrin 3 antibody (ab84422)Image courtesy of Leilani Beltran by Abreview.ab84422 staining Matrin 3 in human U2OS cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde, permeabilized using 0.5% NP-40, blocked with 1% BSA for 1 hour a room temperature, then incubated with ab84422 at a 1/100 dilution for 1 hour. The secondary antibody used was an Alexa-Fluor 488 conjugate donkey polyclonal, used at a 1/1000 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab84422 は 6 報の論文で使用されています。
- You J et al. Selective Loss of MATR3 in Spinal Interneurons, Upper Motor Neurons and Hippocampal CA1 Neurons in a MATR3 S85C Knock-In Mouse Model of Amyotrophic Lateral Sclerosis. Biology (Basel) 11:N/A (2022). PubMed: 35205163
- Cha HJ et al. Inner nuclear protein Matrin-3 coordinates cell differentiation by stabilizing chromatin architecture. Nat Commun 12:6241 (2021). PubMed: 34716321
- Kao CS et al. Selective neuronal degeneration in MATR3 S85C knock-in mouse model of early-stage ALS. Nat Commun 11:5304 (2020). PubMed: 33082323
- Ramesh N et al. RNA dependent suppression of C9orf72 ALS/FTD associated neurodegeneration by Matrin-3. Acta Neuropathol Commun 8:177 (2020). PubMed: 33129345
- Zhao M et al. Knockdown of genes involved in axonal transport enhances the toxicity of human neuromuscular disease-linked MATR3 mutations in Drosophila. FEBS Lett 594:2800-2818 (2020). PubMed: 32515490
- Rajgor D et al. Identification of novel nesprin-1 binding partners and cytoplasmic matrin-3 in processing bodies. Mol Biol Cell 27:3894-3902 (2016). WB, ICC/IF ; Human . PubMed: 27733621