Anti-MATH2/NEUROD6 抗体 (ab85824)
Key features and details
- Rabbit polyclonal to MATH2/NEUROD6
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MATH2/NEUROD6 antibody
MATH2/NEUROD6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MATH2/NEUROD6 -
由来種
Rabbit -
特異性
Replenishment batches of ab85824 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Abpromise guarantee.
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アプリケーション
適用あり: ICC/IFmore details
適用なし: WB -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Monkey
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免疫原
Synthetic peptide corresponding to Human MATH2/NEUROD6 aa 300 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab95917) -
ポジティブ･コントロール
- ICC/IF: U-87 MG cell line.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98.98% PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85824の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
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| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Activates E box-dependent transcription in collaboration with TCF3/E47. May be a trans-acting factor involved in the development and maintenance of the mammalian nervous system. Transactivates the promoter of its own gene. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 540464 Cow
- Entrez Gene: 63974 Human
- Entrez Gene: 11922 Mouse
- Entrez Gene: 500137 Rat
- Omim: 611513 Human
- SwissProt: Q08DI0 Cow
- SwissProt: Q96NK8 Human
- SwissProt: P48986 Mouse
see all -
別名
- Atoh 2 antibody
- Atoh2 antibody
- Atonal homolog 2 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-MATH2/NEUROD6 antibody (ab85824)
ab85824 staining MATH2/NEUROD6 in U-87 MG cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with the antibody ab85824 at 5µg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab85824 は 6 報の論文で使用されています。
- Bhave S et al. Ednrb-/- mice with hirschsprung disease are missing Gad2-expressing enteric neurons in the ganglionated small intestine. Front Cell Dev Biol 10:917243 (2022). PubMed: 35959491
- Komura H et al. Alzheimer Aß Assemblies Accumulate in Excitatory Neurons upon Proteasome Inhibition and Kill Nearby NAKa3 Neurons by Secretion. iScience 13:452-477 (2019). PubMed: 30827871
- Luna E et al. Differential a-synuclein expression contributes to selective vulnerability of hippocampal neuron subpopulations to fibril-induced toxicity. Acta Neuropathol 135:855-875 (2018). PubMed: 29502200
- Sullivan CS et al. Perineuronal Net Protein Neurocan Inhibits NCAM/EphA3 Repellent Signaling in GABAergic Interneurons. Sci Rep 8:6143 (2018). PubMed: 29670169
- Tachibana N et al. Pten Regulates Retinal Amacrine Cell Number by Modulating Akt, Tgfß, and Erk Signaling. J Neurosci 36:9454-71 (2016). PubMed: 27605619
- Klisch TJ et al. In vivo Atoh1 targetome reveals how a proneural transcription factor regulates cerebellar development. Proc Natl Acad Sci U S A 108:3288-93 (2011). ICC/IF ; Mouse . PubMed: 21300888