Anti-Mast Cell Tryptase 抗体 [EPR9522] (ab151757)
![Western blot - Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757)](https://www.abcam.co.jp/ps/products/151/ab151757/Images/ab151757-158878-anti-mast-cell-tryptase-antibody-epr9522-western-blot.jpg "Western blot - Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9522] to Mast Cell Tryptase
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Mast Cell Tryptase antibody [EPR9522]
Mast Cell Tryptase 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR9522] to Mast Cell Tryptase -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human skin, colon cancer or tonsil lysate, Human colon tissue. IP: Human skin lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR9522 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab151757の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
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| IHC-P | (4) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
1/50.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 30 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
1/50. |
ターゲット情報
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関連性
Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Tryptase is considered to be an important marker of mast cell activation as well as an important mediator of inflammation. -
細胞内局在
Secreted. Note: released from the secretory granules upon mast cell activation. -
参照データベース
- Entrez Gene: 7177 Human
- Entrez Gene: 100503895 Mouse
- Entrez Gene: 54271 Rat
- Omim: 191080 Human
- SwissProt: Q15661 Human
- SwissProt: Q02844 Mouse
- SwissProt: P27435 Rat
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別名
- alpha II antibody
- Lung tryptase antibody
- Mast cell alpha II tryptase antibody
see all
画像
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Western blot - Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757)All lanes : Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757) at 1/1000 dilution
Lane 1 : Human skin lysate
Lane 2 : Human colon cancer lysate
Lane 3 : Human tonsil lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 30 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757)](https://www.abcam.co.jp/ps/products/151/ab151757/Images/ab151757-158877-anti-mast-cell-tryptase-antibody-epr9522-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757)Immunohistochemical analysis of paraffin embedded Human tissue labeling Mast Cell Tryptase with ab151757 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757)](https://www.abcam.co.jp/ps/products/151/ab151757/Images/ab151757-5-anti-mast-cell-tryptase-antibody-epr9522-immunoprecipitation-skin-human.jpg)
Immunoprecipitation - Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757)Purified ab151757 at 1/50 dilution (2µg) immunoprecipitating Mast Cell Tryptase in Human skin lysate.
Lane 1 (input): Human skin lysate 10µg
Lane 2 (+): ab151757 + Human skin lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab151757 in Human skin lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 30 kDa -
![Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757)](https://www.abcam.co.jp/ps/products/151/ab151757/Images/ab151757-3-benefits-of-recombinant-antibodies.png)
Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab151757 は 14 報の論文で使用されています。
- Liu J et al. Amyloid Beta Peptides Lead to Mast Cell Activation in a Novel 3D Hydrogel Model. Int J Mol Sci 24:N/A (2023). ICC/IF . PubMed: 37569378
- Chaudhary R et al. EGFR Inhibition by Cetuximab Modulates Hypoxia and IFN Response Genes in Head and Neck Squamous Cell Carcinoma. Cancer Res Commun 3:896-907 (2023). PubMed: 37377902
- Shimbori C et al. Gut bacteria interact directly with colonic mast cells in a humanized mouse model of IBS. Gut Microbes 14:2105095 (2022). PubMed: 35905313
- Atiakshin D et al. Protease profile of normal and neoplastic mast cells in the human bone marrow with special emphasis on systemic mastocytosis. Histochem Cell Biol 155:561-580 (2021). PubMed: 33492488
- Janke LJ et al. Development of Mast Cell and Eosinophil Hyperplasia and HLH/MAS-Like Disease in NSG-SGM3 Mice Receiving Human CD34+ Hematopoietic Stem Cells or Patient-Derived Leukemia Xenografts. Vet Pathol 58:181-204 (2021). PubMed: 33208054