Anti-Mast Cell Tryptase 抗体 [AA1]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (AB2378)

IHC image of Mast Cell Tryptase staining in Human Normal Tonsil formalin fixed paraffin embedded tissue section*.

Performed on a Leica BOND^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. The section was then incubated with ab2378 at 0.05µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (AB2378)

Tryptase-stained skin biopsy.

Skin biopsy in CUrt patient stained for tryptase (ab2378, red) at baseline at low (10×, upper panels) and high (60×, lower panels) magnification and at 15 minutes following cold stimulation time test.

Skin biopsies were obtained at baseline and 15 minutes after CSTT (5 min) on the challenge site, placed into 10% neutral buffered formalin, embedded in paraffin and cut into 5-μm-thick sections. Local anesthesia was performed just before biopsy and did not delay the timing of the biopsy. Slides for immunohistochemistry were deparaffinized and stained. ab2378 was diluted 1 : 100 and incubated for 2 hours. A biotinylated undiluted secondary antibody of Goat anti-Mouse SS Link, was incubated for 32 minutes, followed with enzyme conjugate, and Fast Red chromogen.

Meyer at al PLoS One. 2013;8(2):e56773. doi: 10.1371/journal.pone.0056773. Epub 2013 Feb 22. Fig 2. Reproduced under the Creative Commons license https://creativecommons.org/publicdomain/zero/1.0/

• IHC-P

AbReview9249****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (AB2378)

ab2378 staining formalin fixed paraffin-embedded human colon tissue sections cut at 1 micron.

The section was subjected to heat mediated antigen retrieval and permeabilized in Triton-X prior to blocking in 6% BSA for 2 hours at 24°C. The primary anitbody was diluted 1/250 and incubated with the sample for 16 hours at 5°C. The secondary antibody was ab7064 (diluted 1/2000) and the slide was counterstained with DAPI.

This image is courtesy of an Abreview submitted by Dr Tom Donndelinger

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (AB2378)

AM distribution in thymic mast cells in cryostat sections of human newborn thymic tissue.

Sections were incubated with an antibody against tryptase (A, B) using ab2378 at a 1/100 dilution.

V : blood vessel.

Briefly, frozen sections (10 μm-thick) were cut with a cryostat at −20°C. Antigens were retrieved by heating the sections previously immersed in Bond Epitope Retrieval solution 1 at 100°C for 30 min. Sections were washed in phosphate buffered saline (PBS) and incubated with the primary antibody.

De Martin et al PLoS One. 2014 May 15;9(5):e97592. doi: 10.1371/journal.pone.0097592. eCollection 2014. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• IHC-P

AbReview42462****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (AB2378)

ab2378 staining Mast Cell Tryptase in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (1/7500 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/250) was used as the secondary antibody.

Image is courtesy of an AbReview submitted by Carl Hobbs

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (AB2378)

IHC image of Mast Cell Tryptase staining in Human Normal Skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. The section was then incubated with ab2378, 1/200,000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• IHC-P

AbReview23365****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (AB2378)

ab2378 staining Mast Cell Tryptase in mouse Lung (LPS inflammation) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Tissue was fixed with paraformaldehyde and blocked with 4% M.O.M Ig blocking for 1 hour at 20°C; antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (1/200 in blocking buffer) for 16 hours at 4°C. A Biotin-conjugated Rabbit anti-mouse IgG polyclonal (1/100) was used as the secondary antibody.

Image courtesy of an AbReview submitted by Dr Francois Daubeuf

• IHC-P

AbReview24760****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (AB2378)

ab2378 staining Mast Cell Tryptase in rat Lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

Image is courtesy of an AbReview submitted by Dr Fancois Daubeuf

• WB

Lab

Western blot - Anti-Mast Cell Tryptase antibody [AA1] (AB2378)

Mast Cell Tryptase contains an 18-residue signal sequence and a 12-residue activation peptide. The protein also has two potential glycosylation sites (Swissprot data). These post-translational modifications might explain the banding pattern observed. Abcam used 5% milk in TBS-T as a blocking agent for this blot. We found that this blocking agent removed more non-specific bands than BSA.

Lanes 1 - 3:

Western blot - Anti-Mast Cell Tryptase antibody [AA1] (ab2378) at 1/100 dilution

Lanes 4 - 6:

Western blot - Anti-Mast Cell Tryptase antibody [AA1] (ab2378) at 1/500 dilution

Lanes 1 and 4:

Human lung normal tissue lysate - total protein (40 - 65 years) at 20 µg

Lanes 2 and 5:

Human tonsil normal tissue lysate - total protein at 20 µg

Lanes 3 and 6:

Human skin tissue lysate - total protein at 20 µg

Secondary

All lanes:

Goat Anti-Mouse IgG H&L (HRP) preadsorbed at 1/5000 dilution

Predicted band size: 31 kDa

true

Exposure time: 2min