Anti-Mast Cell Chymase 抗体 [EPR13136] - C-terminal (ab186417)
![Western blot - Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (ab186417)](https://www.abcam.co.jp/ps/products/186/ab186417/Images/ab186417-220448-anti-mast-cell-chymase-antibody-epr13136-c-terminal-western-blot.jpg "Western blot - Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (ab186417)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13136] to Mast Cell Chymase - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal
Mast Cell Chymase 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13136] to Mast Cell Chymase - C-terminal -
由来種
Rabbit -
特異性
Not suitable for mouse or rat samples. Gave high background in IHC-P during initial testing. -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human small intestine tissue lysate and Human colon tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR13136 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186417の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/10000 - 1/50000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
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| IHC-P | (1) |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). |
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. -
組織特異性
Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions. -
配列類似性
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
細胞内局在
Secreted. Cytoplasmic granule. Mast cell granules. - Information by UniProt
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参照データベース
- Entrez Gene: 1215 Human
- Omim: 118938 Human
- SwissProt: P23946 Human
- Unigene: 135626 Human
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別名
- Alpha-chymase antibody
- Chymase 1 antibody
- Chymase 1 mast cell antibody
see all
画像
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Western blot - Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (ab186417)Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (ab186417) at 1/10000 dilution + Human small intestine tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (ab186417)](https://www.abcam.co.jp/ps/products/186/ab186417/Images/ab186417-220447-anti-mast-cell-chymase-antibody-epr13136-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (ab186417)Immunohistochemical analysis of paraffin embedded Human colon tissue labeling Mast Cell Chymase with ab186417 at 1/500 dilution. Secondary staining with HRP Polymer for Rabbit IgG and counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (ab186417)](https://www.abcam.co.jp/ps/products/186/ab186417/Images/ab186417-3-benefits-of-recombinant-antibodies.png)
Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (ab186417)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (9)
ab186417 は 9 報の論文で使用されています。
- Butts B et al. Impact of early pericardial fluid chymase activation after cardiac surgery. Front Cardiovasc Med 10:1132786 (2023). PubMed: 37265571
- Fan Z et al. Immune-related SERPINA3 as a biomarker involved in diabetic nephropathy renal tubular injury. Front Immunol 13:979995 (2022). PubMed: 36304455
- Ud-Din S et al. Pre-Emptive Priming of Human Skin Improves Cutaneous Scarring and Is Superior to Immediate and Delayed Topical Anti-Scarring Treatment Post-Wounding: A Double-Blind Randomised Placebo-Controlled Clinical Trial. Pharmaceutics 13:N/A (2021). PubMed: 33917842
- Garcia-Rodriguez KM et al. Differential mast cell numbers and characteristics in human tuberculosis pulmonary lesions. Sci Rep 11:10687 (2021). PubMed: 34021178
- Jitmana R et al. Role of cardiac mast cells in exercise training-mediated cardiac remodeling in angiotensin II-infused ovariectomized rats. Life Sci 219:209-218 (2019). PubMed: 30658099
- Ud-Din S et al. A Double-Blind, Randomized Trial Shows the Role of Zonal Priming and Direct Topical Application of Epigallocatechin-3-Gallate in the Modulation of Cutaneous Scarring in Human Skin. J Invest Dermatol 139:1680-1690.e16 (2019). PubMed: 30822414
- Bahri R et al. Mast cell activation test in the diagnosis of allergic disease and anaphylaxis. J Allergy Clin Immunol 142:485-496.e16 (2018). PubMed: 29518421
- Akyol GY et al. IVIG activates Fc?RIIB-SHIP1-PIP3 Pathway to stabilize mast cells and suppress inflammation after ICH in mice. Sci Rep 7:15583 (2017). PubMed: 29138419
- Sun N et al. Angiotensin-Converting Enzyme Inhibitor (ACEI)-Mediated Amelioration in Renal Fibrosis Involves Suppression of Mast Cell Degranulation. Kidney Blood Press Res 41:108-18 (2016). PubMed: 26881856