Anti-Mast Cell Chymase 抗体 (ab111239)
Key features and details
- Goat polyclonal to Mast Cell Chymase
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-Mast Cell Chymase antibody
Mast Cell Chymase 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Mast Cell Chymase -
由来種
Goat -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Mast Cell Chymase aa 50-150 (internal sequence) (Cysteine residue).
-
ポジティブ･コントロール
- Human testis and tonsil tissue
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab111239の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 1.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
IHC-P
Use a concentration of 1.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. -
組織特異性
Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions. -
配列類似性
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
細胞内局在
Secreted. Cytoplasmic granule. Mast cell granules. - Information by UniProt
-
参照データベース
- Entrez Gene: 1215 Human
- Omim: 118938 Human
- SwissProt: P23946 Human
- Unigene: 135626 Human
-
別名
- Alpha-chymase antibody
- Chymase 1 antibody
- Chymase 1 mast cell antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody (ab111239)ab111239, at 1.5 µg/ml, staining Mast Cell Chymase in formalin fixed, paraffin embedded Human testis tissue by Immunohistochemistry. Biotinylated secondary antibody, followed by alkaline phosphatase streptavidin and chromogen were used to develop staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody (ab111239)ab111239, at 1.5 µg/ml, staining Mast Cell Chymase in formalin fixed, paraffin embedded Human tonsil tissue by Immunohistochemistry. Biotinylated secondary antibody, followed by alkaline phosphatase streptavidin and chromogen were used to develop staining.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (9)
ab111239 は 9 報の論文で使用されています。
- Lapointe C et al. Chymase Inhibition Resolves and Prevents Deep Vein Thrombosis Without Increasing Bleeding Time in the Mouse Model. J Am Heart Assoc 12:e028056 (2023). PubMed: 36752268
- Wu HJ et al. Spatial intra-tumor heterogeneity is associated with survival of lung adenocarcinoma patients. Cell Genom 2:N/A (2022). PubMed: 36419822
- Yamanaka-Takaichi M et al. Stress and Nasal Allergy: Corticotropin-Releasing Hormone Stimulates Mast Cell Degranulation and Proliferation in Human Nasal Mucosa. Int J Mol Sci 22:N/A (2021). PubMed: 33803422
- Kovacs M et al. The pathogenic role of c-Kit+ mast cells in the spinal motor neuron-vascular niche in ALS. Acta Neuropathol Commun 9:136 (2021). PubMed: 34389060
- Trias E et al. Schwann cells orchestrate peripheral nerve inflammation through the expression of CSF1, IL-34, and SCF in amyotrophic lateral sclerosis. Glia 68:1165-1181 (2020). PubMed: 31859421
- Valero-Jiménez A et al. Transmembrane protease, serine 4 (TMPRSS4) is upregulated in IPF lungs and increases the fibrotic response in bleomycin-induced lung injury. PLoS One 13:e0192963 (2018). PubMed: 29529050
- Trias E et al. Mast cells and neutrophils mediate peripheral motor pathway degeneration in ALS. JCI Insight 3:N/A (2018). PubMed: 30282815
- Judd LM et al. Elevated IL-33 expression is associated with pediatric eosinophilic esophagitis, and exogenous IL-33 promotes eosinophilic esophagitis development in mice. Am J Physiol Gastrointest Liver Physiol 310:G13-25 (2016). Mouse . PubMed: 26514775
- Buzzelli JN et al. IL33 Is a Stomach Alarmin That Initiates a Skewed Th2 Response to Injury and Infection. Cell Mol Gastroenterol Hepatol 1:203-221.e3 (2015). PubMed: 28210674