Anti-MASPIN 抗体 (ab65136)
Key features and details
- Rabbit polyclonal to MASPIN
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MASPIN antibody
MASPIN 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MASPIN -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
A synthesized peptide derived from an internal region of human MASPIN
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ポジティブ･コントロール
- Extracts from Hela cells WB: HeLa, HaCaT, Caco-2 cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.4 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab65136の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 5 µg/ml.
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| WB |
1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 1 - 5 µg/ml. |
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WB
1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). |
ターゲット情報
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機能
Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. -
組織特異性
Normal mammary epithelial cells. -
配列類似性
Belongs to the serpin family. Ov-serpin subfamily. -
細胞内局在
Secreted > extracellular space. - Information by UniProt
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参照データベース
- Entrez Gene: 5268 Human
- Entrez Gene: 20724 Mouse
- Entrez Gene: 116589 Rat
- Omim: 154790 Human
- SwissProt: P36952 Human
- SwissProt: P70124 Mouse
- SwissProt: P70564 Rat
- Unigene: 55279 Human
see all -
別名
- Maspin antibody
- Ovalbumin antibody
- Peptidase inhibitor 5 antibody
see all
画像
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Western blot - Anti-MASPIN antibody (ab65136)All lanes : Anti-MASPIN antibody (ab65136) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SERPINB5 knockout HeLa cell lysate
Lane 3 : HaCaT cell lysate
Lane 4 : Caco-2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDaLanes 1 - 4: Merged signal (red and green). Green - ab65136 observed at 42 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab65136 was shown to react with MASPIN in wild-type cells in Western blot with loss of signal observed in SERPINB5 knockout cell line ab264750 (SERPINB5 knockout cell lysate ab258659). Wild-type and SERPINB5 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with ab65136 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1/500 dilution and a 1/20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 h at room temperature before imaging.
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Western blot - Anti-MASPIN antibody (ab65136)All lanes : Anti-MASPIN antibody (ab65136) at 1/500 dilution
Lane 1 : Extracts from Hela cells
Lane 2 : Extracts from Hela cells, plus immunising peptide
Predicted band size: 42 kDa
Observed band size: 42 kDa
The amount of positive control loading for the WB is 5-30 ug of total protein.The amount of the peptide for the WB is 5-10 ug. -
Immunocytochemistry/ Immunofluorescence - Anti-MASPIN antibody (ab65136)ICC/IF image of ab65136 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65136, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab65136 は 1 報の論文で使用されています。
- Todd AG et al. SMN, Gemin2 and Gemin3 associate with beta-actin mRNA in the cytoplasm of neuronal cells in vitro. J Mol Biol 401:681-9 (2010). PubMed: 20620147
