Anti-MASH1/Achaete-scute homolog 1 抗体 [EPR19840] (ab211327)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)](https://www.abcam.co.jp/ps/products/211/ab211327/Images/ab211327-278392-anti-mash1-achaete-scute-homolog-1-antibody-epr19840-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19840] to MASH1/Achaete-scute homolog 1
- Suitable for: ChIP-sequencing, IHC-P, WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840]
MASH1/Achaete-scute homolog 1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR19840] to MASH1/Achaete-scute homolog 1 -
由来種
Rabbit -
アプリケーション
適用あり: ChIP-sequencing, IHC-P, WB, ICC/IF, IPmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Mouse medullary thyroid cancer lysate; NCI-H69 whole cell lysate. IHC-P: Mouse small cell lung cancer section; Ascl1CreER/+; ROSA26mTmG/+ mouse thyroid tissue. ICC/IF: NCI-H69 cells. IP: Mouse medullary thyroid cancer lysate; NCI-H69 whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR19840 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab211327の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ChIP-sequencing |
Use 8µg for 107 cells.
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| IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa).
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| ICC/IF | (2) |
1/100.
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| IP |
1/30.
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| 特記事項 |
|---|
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ChIP-sequencing
Use 8µg for 107 cells. |
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IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa). |
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ICC/IF
1/100. |
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IP
1/30. |
ターゲット情報
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機能
May play a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Activates transcription by binding to the E box (5'-CANNTG-3'). -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 429 Human
- Entrez Gene: 17172 Mouse
- Omim: 100790 Human
- SwissProt: P50553 Human
- SwissProt: Q02067 Mouse
- Unigene: 703025 Human
- Unigene: 136217 Mouse
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別名
- Achaete scute complex homolog 1 antibody
- Achaete scute complex homolog like 1 antibody
- Achaete scute complex homologue 1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)
Immunohistochemical analysis of 4% paraformaldehyde-fixed mouse small cell lung cancer section labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 dilution, followed by Donkey anti Rabbit Alexa-594 at 1/500 dilution (red).
Nuclear counterstain: DAPI (blue).
Positive staining of MASH1/Achaete-scute homolog 1 on mouse small cell lung cancer.
The image was kindly provided by our collaborator Dr. Hai Song, Zhejiang University.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)](https://www.abcam.co.jp/ps/products/211/ab211327/Images/ab211327-278391-anti-mash1-achaete-scute-homolog-1-antibody-epr19840-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)Immunohistochemical analysis of 4% paraformaldehyde-fixed Ascl1CreER/+; ROSA26mTmG/+ mouse thyroid tissue labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 dilution, followed by Tyramide Signal Amplification (red).
Counterstain: DAPI (blue) and chick anti-GFP (green).
ROSA26mTmG/+ mice were bred with Ascl1CreER/+ mice, in which the Ascl1 promoter drives the expression of Cre, to generate mice carrying the genotype of Ascl1CreER/+; ROSA26mTmG/+. Cre activation upon Tamoxifen administration resulted in eGFP expression from the ROSA26mTmG/+ allele. The thyroid tissue from Ascl1CreER/+; ROSA26mTmG/+ mouse was fixed and embedded in paraffin. Paraffin section was stained with rabbit anti-MASH1/Achaete-scute homolog 1 (ab211327) antibody (Red color) and chick anti-GFP antibody (Green color). Tyramide signal amplification was used for high-resolution imaging of low-abundance targets.
The image was kindly provided by our collaborator Dr. Hai Song, Zhejiang University.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)](https://www.abcam.co.jp/ps/products/211/ab211327/Images/ab211327-278393-anti-mash1-achaete-scute-homolog-1-antibody-epr19840-western-blot.jpg)
Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)All lanes : Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327) at 1/1000 dilution
Lane 1 : Mouse medullary thyroid cancer lysate at 20 µg
Lane 2 : NCI-H69 (Human small cell lung cancer cell line) whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 25 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 15 seconds; Lane 2: 10 seconds
Ascl1 is highly expressed in medullary thyroid cancer and small cell lung cancer (PMID: 17090547 and 25267614). Our results showed specific band on mouse medullary thyroid cancer lysate and NCI-H69 (Human small cell lung cancer) lysate. The lysates were kindly provided by our collaborator Dr. Hai Song, Zhejiang University.
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![Immunocytochemistry/ Immunofluorescence - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)](https://www.abcam.co.jp/ps/products/211/ab211327/Images/ab211327-278390-anti-mash1-achaete-scute-homolog-1-antibody-epr19840-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.5% Triton X-100 permeabilized NCI-H69 Human small cell lung cancer cells labeling MASH1/Achaete-scute homolog 1 with ab211327 at 1/100 dilution (red).
The nuclear counterstain is DAPI (blue).
Nuclear MASH1/Achaete-scute homolog 1 staining on NCI-H69 cells.
The images were kindly provided by our collaborator Dr. Hai Song, Zhejiang University.
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![Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)](https://www.abcam.co.jp/ps/products/211/ab211327/Images/ab211327-278389-anti-mash1-achaete-scute-homolog-1-antibody-epr19840-immunoprecipitation.jpg)
Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)MASH1/Achaete-scute homolog 1 was immunoprecipitated from 0.35 mg of mouse medullary thyroid cancer lysate with ab211327 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211327 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
Lane 1: Mouse medullary thyroid cancer lysate 10 μg (Input).
Lane 2: ab211327 IP in mouse medullary thyroid cancer lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211327 in mouse medullary thyroid cancer lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)](https://www.abcam.co.jp/ps/products/211/ab211327/Images/ab211327-278388-anti-mash1-achaete-scute-homolog-1-antibody-epr19840-immunoprecipitation.jpg)
Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)MASH1/Achaete-scute homolog 1 was immunoprecipitated from 0.35 mg of NCI-H69 (Human small cell lung cancer cell line) whole cell lysate with ab211327 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211327 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
Lane 1: NCI-H69 whole cell lysate 10 μg (Input).
Lane 2: ab211327 IP in NCI-H69 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211327 in NCI-H69 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)](https://www.abcam.co.jp/ps/products/211/ab211327/Images/ab211327-7-benefits-of-recombinant-antibodies.png)
Anti-MASH1/Achaete-scute homolog 1 antibody [EPR19840] (ab211327)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (21)
ab211327 は 21 報の論文で使用されています。
- Xie Y et al. New AAV tools fail to detect Neurod1-mediated neuronal conversion of Müller glia and astrocytes in vivo. EBioMedicine 90:104531 (2023). PubMed: 36947961
- Xiao M et al. Skin Wound following Irradiation Aggravates Radiation-Induced Brain Injury in a Mouse Model. Int J Mol Sci 24:N/A (2023). PubMed: 37445879
- Vogt M et al. c-Cbl Regulates Murine Subventricular Zone-Derived Neural Progenitor Cells in Dependence of the Epidermal Growth Factor Receptor. Cells 12:N/A (2023). PubMed: 37830613
- Zhao X et al. 4D imaging analysis of the aging mouse neural stem cell niche reveals a dramatic loss of progenitor cell dynamism regulated by the RHO-ROCK pathway. Stem Cell Reports 17:245-258 (2022). PubMed: 35030320
- Huang S et al. In vivo development and single-cell transcriptome profiling of human brain organoids. Cell Prolif 55:e13201 (2022). PubMed: 35141969