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AB322665

Anti-MASH1/Achaete-scute homolog 1 抗体 [RM1182]

Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182]

  • 20ul selling size
  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • Advanced Validation
  • 詳細を見る

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Rabbit Recombinant Multiclonal MASH1/Achaete-scute homolog 1 antibody. Suitable for WB, ChIP-seq, IHC-P, IP and reacts with Human, Mouse, Rat samples.

別名を表示する

ASH1, BHLHA46, HASH1, ASCL1, Achaete-scute homolog 1, ASH-1, hASH1, Class A basic helix-loop-helix protein 46, bHLHa46, Ash1, Mash-1, Mash1, Ascl1, Achaete-scute homolog 1, ASH-1, mASH-1, mASH1

11 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (AB322665)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (AB322665)

Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling MASH1/Achaete-scute homolog 1 with ab322665 at 1/50 (10.42 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on human thyroid carcinoma. The section was incubated with ab322665 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (AB322665)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (AB322665)

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling MASH1/Achaete-scute homolog 1 with ab322665 at 1/50 (10.42 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on human lung carcinoma. The section was incubated with ab322665 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (AB322665)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (AB322665)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling MASH1/Achaete-scute homolog 1 with ab322665 at 1/50 (10.42 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Negative control : no staining on human kidney. The section was incubated with ab322665 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (AB322665)
  • ChIP-seq

Supplier Data

ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (AB322665)

Chromatin was prepared from NCI-H69 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 cells and 8 µg of ab322665 [RM1182]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (AB322665)
  • ChIP-seq

Supplier Data

ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (AB322665)

Chromatin was prepared from NCI-H69 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 cells and 8 µg of ab322665 [RM1182]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (AB322665)
  • IP

Supplier Data

Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (AB322665)

MASH1/Achaete-scute homolog 1 was immunoprecipitated from 0.35 mg NCI-H69 (human lung small cell carcinoma cell line) whole cell lysate with ab322665 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab322665 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : NCI-H69 (human lung small cell carcinoma cell line) whole cell lysate
Lane 2 : ab322665 IP in NCI-H69 (human lung small cell carcinoma cell line) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab322665 in NCI-H69 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (ab322665) at 1/30 dilution

All lanes:

NCI-H69 (human lung small cell carcinoma cell line) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 76s

ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (AB322665)
  • ChIP-seq

Supplier Data

ChIP-sequencing - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (AB322665)

Chromatin was prepared from NCI-H69 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 cells and 8 µg of ab322665 [RM1182]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (AB322665)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (AB322665)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling MASH1/Achaete-scute homolog 1 with ab322665 at 1/100 (5.21 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on rat cerebrum. The section was incubated with ab322665 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (AB322665)
  • WB

Supplier Data

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (AB322665)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In lane 2, the lysate was stored at -80℃ prior to Western Blotting. The bands beneath the target band (30 kDa) are likey to be degradation products. In lane 1/3, To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

Exposure time : Lanes 1-2 : 180 seconds; Lane 3 : 59 seconds

All lanes:

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (ab322665) at 1/1000 dilution

Lanes 1 - 2:

NCI-H69 (human lung small cell carcinoma cell line) whole cell lysate at 20 µg

Lane 3:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 30 kDa

false

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (AB322665)
  • WB

Supplier Data

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (AB322665)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : A549 (PMID : 31231131).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (ab322665) at 1/1000 dilution

Lane 1:

NCI-H69 (human lung small cell carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 30 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (AB322665)
  • WB

Supplier Data

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (AB322665)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : kidney

The bands beneath the target band (30 kDa) are likey to be degradation products.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody [RM1182] (ab322665) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Human kidney tissue lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Lane 4:

Mouse kidney tissue lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Lane 6:

Rat kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 30 kDa,36 kDa

false

Exposure time: 180s

Key facts

宿主種

Rabbit

クローン性

Multiclonal

クローン番号

RM1182

アイソタイプ

IgG

キャリアフリー

No

交差種

Human, Mouse, Rat

アプリケーション

IP, IHC-P, ChIP-seq, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

Unsuitable for mouse IHC-P, mouse/rat IP.

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組合せ製品

Sample Prep & Detection Kits

AB185913

High-Sensitivity ChIP Kit

ChIP - High-Sensitivity ChIP Kit (AB185913)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ChIPseq" : {"fullname" : "ChIP-sequencing", "shortname":"ChIP-seq"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ChIPseq-species-checked": "testedAndGuaranteed", "ChIPseq-species-dilution-info": "8 µg for 10^7 Cells", "ChIPseq-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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製品の詳細

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Transcription factor that plays a key role in neuronal differentiation : acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Involved in the regulation of neuroendocrine cell development in the glandular stomach (By similarity).
See full target information ASCL1
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Abcam product promise

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