Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) 抗体 [EPR5463] (ab126731)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5463] to MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215)
- Suitable for: Indirect ELISA, WB, Dot blot
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] -
製品の詳細
Rabbit monoclonal [EPR5463] to MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) -
由来種
Rabbit -
特異性
This antibody detects MARK4 phosphorylated at Threonine 214, MARK2 phosphorylated at Threonine 208, MARK3 phosphorylated at Threonine 234 and MARK1 phosphorylated at Threonine 215.
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アプリケーション
適用あり: Indirect ELISA, WB, Dot blotmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 1.62 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5463 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab126731の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Indirect ELISA |
Use a concentration of 1000 µg/ml.
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WB |
1/10000 - 1/50000. Detects a band of approximately 110 kDa (predicted molecular weight: 89 kDa).
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Dot blot |
1/1000.
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特記事項 |
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Indirect ELISA
Use a concentration of 1000 µg/ml. |
WB
1/10000 - 1/50000. Detects a band of approximately 110 kDa (predicted molecular weight: 89 kDa). |
Dot blot
1/1000. |
ターゲット情報
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細胞内局在
MARK4: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, cilium axoneme. Cytoplasm. Localized at the tips of neurite-like processes in differentiated neuroblast cells. Detected in the cytoplasm and neuropil of the hippocampus. MARK2: Cell membrane. Phosphorylated by PRKCZ in polarized epithelial cells, resulting in an interaction with YWHAZ which promotes relocation from the lateral to the apical membrane. MARK1: Cytoplasm > cytoskeleton. Appears to localize to an intracellular network. -
参照データベース
- Entrez Gene: 2011 Human
- Entrez Gene: 4139 Human
- Entrez Gene: 57787 Human
- Entrez Gene: 13728 Mouse
- Entrez Gene: 226778 Mouse
- Entrez Gene: 232944 Mouse
- Entrez Gene: 117016 Rat
- Entrez Gene: 60328 Rat
see all
画像
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Western blot - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (ab126731)
Westernblot analysis of ab126731 at a 1/20 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as a scondary antibody at 1/20000 dilution.
Lane 1: HEK-293 (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate 15μg
Lane 2: HEK-293 transfected with MARK1 expression vector containing a myc-His-tag®, whole cell lysate 15μg
Lane 3: HEK-293 transfected with MARK1 expression vector containing a myc-His-tag®, whole cell lysate 15μg, then the membrane treated with Alkaline Phosphatase for 1 hour
Lane 4: HEK-293 transfected with MARK2 expression vector containing a myc-His-tag®, whole cell lysate 15μg
Lane 5: HEK-293 transfected with MARK2 expression vector containing a myc-His-tag®, whole cell lysate 15μg, then the membrane treated with Alkaline Phosphatase for 1 hour
Lane 6: HEK-293 transfected with MARK3 expression vector containing a myc-His-tag®, whole cell lysate 15μg
Lane 7: HEK-293 transfected with MARK3 expression vector containing a myc-His-tag®, whole cell lysate 15μg, then the membrane treated with Alkaline Phosphatase for 1 hour
Lane 8: HEK-293 transfected with MARK4 expression vector containing a myc-His-tag®, whole cell lysate 15μg
Lane 9: HEK-293 transfected with MARK4 expression vector containing a myc-His-tag®, whole cell lysate 15μg, then the membrane treated with Alkaline Phosphatase for 1 hour
Exposure time:
Lane 1 – 3, 6 - 9: 180 seconds
Lane 4 - 5: 7 seconds
Endogenous MARK proteins are observed at ~110 kDa reported by PMID: 9108484 and 16238695
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Indirect ELISA - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (ab126731)
Indirect ELISA antibody dose-response curve using ab126731 between 0-10000 ng/ml. Antigen concentration of 500 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
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Dot Blot - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (ab126731)
Dot Blot using ab126731 at a 1/1000 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at a 1/100000 dilution
Lane 1: Human MARK1 (T215) phospho peptide
Lane 2: Human MARK1 non-phospho peptide
Lane 3: Human MARK2 (T208) phospho peptide
Lane 4: Human MARK2 non-phospho peptide
Lane 5: Human MARK3 (T234) phospho peptide
Lane 6: Human MARK3 non-phospho peptide
Lane 7: Human MARK4 (T214) phospho peptide
Lane 8: Human MARK4 non-phospho peptide
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Western blot - Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antibody [EPR5463] (ab126731)All lanes : Anti-MARK4 (phospho T214) + MARK2 (phospho T208) + MARK3 (phospho T234) + MARK1 (phospho T215) antib (ab126731) at 1/10000 dilution
Lane 1 : SH-SY5Y cell lysate, untreated
Lane 2 : SH-SY5Y cell lysate, treated with Okadaic acid and Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 89 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab126731 は論文での使用が確認できていません。