Anti-MARK2 (phospho T595) 抗体 (ab34751)
Key features and details
- Rabbit polyclonal to MARK2 (phospho T595)
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
製品の概要
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製品名
Anti-MARK2 (phospho T595) antibody
MARK2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MARK2 (phospho T595) -
由来種
Rabbit -
特異性
MARK2 pT595 affinity purified antibody is directed against the phosphorylated form of human MARK2 at the pT595 residue. Reactivity occurs against human MARK2 pT595 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human MARK2 is minimal by ELISA. Expect at least partial reactivity with other isoforms of MARK. The immunogen sequence is present in all forms identified to date and localizes to T595 on MARK2, T587 on MARK3, T591 on MARK1 and T568 on MARK4. A BLAST analysis was used to suggest reactivity with this protein from human, dog, bovine, mouse and rat sources based on 100% homology for the immunogen sequence. Cross reactivity with MARK2 homologues from other sources has not been determined.
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Recombinant fragment
交差が予測される動物種: Mouse, Cow, Dog
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免疫原
Synthetic peptide corresponding to Human MARK2 aa 550-650 (phospho T595).
Database link: Q7KZI7 -
ポジティブ･コントロール
- HEK293 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
MARK2 pT595 affinity purified antibody is directed against the phosphorylated form of human MARK2 at the pT595 residue. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab34751の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/250 - 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 87 kDa).
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| 特記事項 |
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WB
1/250 - 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 87 kDa). |
ターゲット情報
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機能
Role in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection. -
組織特異性
High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain. -
細胞内局在
Cell membrane. Phosphorylated by PRKCZ in polarized epithelial cells, resulting in an interaction with YWHAZ which promotes relocation from the lateral to the apical membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 535197 Cow
- Entrez Gene: 483769 Dog
- Entrez Gene: 13728 Mouse
- SwissProt: Q05512 Mouse
- Unigene: 258986 Mouse
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別名
- ELKL motif kinase 1 antibody
- ELKL motif kinase antibody
- EMK-1 antibody
see all
画像
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Western blot - Anti-MARK2 (phospho T595) antibody (ab34751)All lanes : Anti-MARK2 (phospho T595) antibody (ab34751) at 1/500 dilution
Lane 1 : MW markers
Lane 2 : untransfected HEK293 cell lysate
Lane 3 : HEK293 cells transfected with pCMV-3×Flag-
MARK2 plus pCMV-Flag-PKC-zeta
Lane 4 : HEK293 cells transfected with pCMV-3×Flag-MARK2 only
Predicted band size: 87 kDa
Observed band size: 82 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab34751 は 5 報の論文で使用されています。
- Lu YN et al. MARK2 phosphorylates eIF2a in response to proteotoxic stress. PLoS Biol 19:e3001096 (2021). PubMed: 33705388
- Mirza AN et al. Combined inhibition of atypical PKC and histone deacetylase 1 is cooperative in basal cell carcinoma treatment. JCI Insight 2:N/A (2017). Mouse . PubMed: 29093271
- Yadav SK et al. Regulation of aPKC activity by Nup358 dependent SUMO modification. Sci Rep 6:34100 (2016). WB ; Human . PubMed: 27682244
- Tobias IS & Newton AC Protein Scaffolds Control Localized Protein Kinase C? Activity. J Biol Chem 291:13809-22 (2016). PubMed: 27143478
- Wu-Zhang AX et al. Cellular Pharmacology of Protein Kinase M? (PKM?) Contrasts with Its in Vitro Profile: IMPLICATIONS FOR PKM? AS A MEDIATOR OF MEMORY. J Biol Chem 287:12879-85 (2012). WB ; Rat . PubMed: 22378786