Anti-MARCO antibody [RM1219]
Anti-MARCO antibody [RM1219]
- BOND RX™ Validated
- RabMAb
- Recombinant
- 20ul selling size
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Rabbit Recombinant Multiclonal MARCO antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.
別名を表示する
Macrophage receptor MARCO, Macrophage receptor with collagenous structure, Marco, SCARA2, MARCO, Macrophage receptor MARCO, Macrophage receptor with collagenous structure, Scavenger receptor class A member 2
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling MARCO with ab323717 at 1/200 (2.49 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on the Kupffer cells in human liver. The section was incubated with ab323717 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling MARCO with ab323717 at 1/200 (2.49 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control : no staining in human pancreas. The section was incubated with ab323717 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [RM1219] (AB323717)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized Human PBMC (human primary peripheral blood mononuclear cell) cells labelling MARCO with ab323717 at 1/500 (0.996 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).
Confocal image showing the cytoplasmic and membranous staining is increased in subsets of human PBMCs (shown in green) treated with 50 ng/ml M-CSF for 72 hours, then add 20 ng/ml IL-10 for 24 hours. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.
- mIHC
Supplier Data
Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human liver tissue staining MARCO with ab323717 at a 1 : 200 (2.49 ug/ml) dilution, ab213363 anti-CD68 used at 1 : 500 (1.26 ug/ml) dilution and ab237721 anti-CD3 used at a 1 : 2000 (0.26 ug/ml) dilution.
Panel A : merged staining of anti-MARCO (green; Opal™520), anti-CD68 (magenta; Opal™570) and anti-CD3 (yellow; Opal™690) on human liver.
Panel B : anti-MARCO staining macrophages in human liver.
Panel C : ant-CD68 staining macrophages in human liver.
Panel D : ant-CD3 staining T lymphocytes in human liver.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining : in the order of ab323717, ab213363 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Nuclear counter stain with DAPI.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)
Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling MARCO with ab323717 at 1/200 (2.49 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on the macrophages in human lung. The section was incubated with ab323717 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- mIHC
Supplier Data
Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human liver tissue staining MARCO with ab323717 at a 1 : 200 (2.49 ug/ml) dilution, ab320735 anti-CD19 used at 1 : 2000 (0.255 ug/ml) dilution and ab237721 anti-CD3 used at a 1 : 2000 (0.26 ug/ml) dilution.
Panel A : merged staining of anti-MARCO (green; Opal™520), anti-CD19 (gray; Opal™570) and anti-CD3 (magenta; Opal™690) on human liver.
Panel B : anti-MARCO staining macrophages in human liver.
Panel C : ant-CD19 staining B lymphocytes in human liver.
Panel D : ant-CD3 staining T lymphocytes in human liver.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining : in the order of ab323717, ab320735 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Nuclear counter stain with DAPI.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
- mIHC
Supplier Data
Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse spleen tissue staining MARCO with ab323717 at a 1 : 200 (2.49 ug/ml) dilution, ab245235 anti-CD19 used at 1 : 1000 (0.22 ug/ml) dilution and ab237721 anti-CD3 used at a 1 : 2000 (0.26 ug/ml) dilution.
Panel A : merged staining of anti-MARCO (green; Opal™520), anti-CD19 (gray; Opal™690) and anti-CD3 (magenta; Opal™570) on mouse spleen.
Panel B : anti-MARCO staining macrophages in mouse spleen.
Panel C : ant-CD19 staining B lymphocytes in mouse spleen.
Panel D : ant-CD3 staining T lymphocytes in mouse spleen.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining : in the order of ab323717, ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Nuclear counter stain with DAPI.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
- mIHC
Supplier Data
Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse liver tissue staining MARCO with ab323717 at a 1 : 200 (2.49 ug/ml) dilution, ab245235 anti-CD19 used at 1 : 1000 (0.22 ug/ml) dilution and ab237721 anti-CD3 used at a 1 : 2000 (0.26 ug/ml) dilution.
Panel A : merged staining of anti-MARCO (green; Opal™520), anti-CD19 (gray; Opal™690) and anti-CD3 (magenta; Opal™570) on mouse liver.
Panel B : anti-MARCO staining macrophages in mouse liver.
Panel C : ant-CD19 staining B lymphocytes in mouse liver.
Panel D : ant-CD3 staining T lymphocytes in mouse liver.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining : in the order of ab323717, ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Nuclear counter stain with DAPI.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [RM1219] (AB323717)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized J774A.1(mouse reticulum cell sarcoma monocyte/macrophage) cells labelling MARCO with ab323717 at 1/500 (0.996 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).
Confocal image showing increased cytoplasmic staining in J774A.1 cells treated with 1 ng/ml lipopolysaccharide (LPS) for 24 hours (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling MARCO with ab323717 at 1/400 (1.245 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Positive staining on the Kupffer cells in rat liver. The primary antibody was incubated overnight at 4°C.
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)
Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling MARCO with ab323717 at 1/400 (1.245 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Positive staining on the macrophages in rat lung. The primary antibody was incubated overnight at 4°C.
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling MARCO with ab323717 at 1/200 (2.49 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on the Kupffer cells in mouse liver. The section was incubated with ab323717 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- mIHC
Supplier Data
Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse lung tissue staining MARCO with ab323717 at a 1 : 200 (2.49 ug/ml) dilution, ab245235 anti-CD19 used at 1 : 1000 (0.22 ug/ml) dilution and ab237721 anti-CD3 used at a 1 : 2000 (0.26 ug/ml) dilution.
Panel A : merged staining of anti-MARCO (green; Opal™520), anti-CD19 (gray; Opal™690) and anti-CD3 (magenta; Opal™570) on mouse lung.
Panel B : anti-MARCO staining macrophages in mouse lung.
Panel C : ant-CD19 staining B lymphocytes in mouse lung.
Panel D : ant-CD3 staining T lymphocytes in mouse lung.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining : in the order of ab323717, ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Nuclear counter stain with DAPI.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling MARCO with ab323717 at 1/200 (2.49 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on the macrophages in mouse lung. The section was incubated with ab323717 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- mIHC
Supplier Data
Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat liver tissue staining MARCO with ab323717 at a 1 : 200 (2.49 ug/ml) dilution, ab283654 anti-CD68 used at 1 : 100 (4.43 ug/ml) dilution and ab237721 anti-CD3 used at a 1 : 2000 (0.26 ug/ml) dilution.
Panel A : merged staining of anti-MARCO (green; Opal™520), anti-CD68 (magenta; Opal™690) and anti-CD3 (yellow; Opal™570) on rat liver.
Panel B : anti-MARCO staining macrophages in rat liver.
Panel C : ant-CD68 staining macrophages in rat liver.
Panel D : ant-CD3 staining T lymphocytes in rat liver.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining : in the order of ab323717, ab283654 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Nuclear counter stain with DAPI.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
- mIHC
Supplier Data
Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse lung tissue staining MARCO with ab323717 at a 1 : 200 (2.49 ug/ml) dilution, ab283654 anti-CD68 used at 1 : 100 (4.43 ug/ml) dilution and ab237721 anti-CD3 used at a 1 : 2000 (0.26 ug/ml) dilution.
Panel A : merged staining of anti-MARCO (green; Opal™520), anti-CD68 (magenta; Opal™690) and anti-CD3 (yellow; Opal™570) on mouse lung.
Panel B : anti-MARCO staining macrophages in mouse lung.
Panel C : ant-CD68 staining macrophages in mouse lung.
Panel D : ant-CD3 staining T lymphocytes in mouse lung.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining : in the order of ab323717, ab283654 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Nuclear counter stain with DAPI.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)
Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling MARCO with ab323717 at 1/200 (2.49 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control : no staining in mouse pancreas. The section was incubated with ab322705 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)
Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling MARCO with ab323717 at 1/400 (1.245 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Negative control : no staining in rat pancreas. The primary antibody was incubated overnight at 4°C.
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)
- mIHC
Supplier Data
Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat liver tissue staining MARCO with ab323717 at a 1 : 200 (2.49 ug/ml) dilution, ab64088 anti-CD20 used at 1 : 100 (2.91 ug/ml) dilution and ab237721 anti-CD3 used at a 1 : 2000 (0.26 ug/ml) dilution.
Panel A : merged staining of anti-MARCO (green; Opal™520), anti-CD20 (gray; Opal™690) and anti-CD3 (magenta; Opal™570) on rat liver.
Panel B : anti-MARCO staining macrophages in rat liver.
Panel C : ant-CD20 staining B lymphocytes in rat liver.
Panel D : ant-CD3 staining T lymphocytes in rat liver.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining : in the order of ab323717, ab64088 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Nuclear counter stain with DAPI.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
- WB
Supplier Data
Western blot - Anti-MARCO antibody [RM1219] (AB323717)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
Negative control : pancreas.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
All lanes:
Western blot - Anti-MARCO antibody [RM1219] (ab323717) at 1/1000 dilution
Lane 1:
Human spleen tissue lysate at 40 µg
Lane 2:
Human pancreas tissue lysate at 40 µg
Lane 3:
Human lung tissue lysate at 40 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 75 kDa,36 kDa
false
Exposure time: 180s
- WB
Supplier Data
Western blot - Anti-MARCO antibody [RM1219] (AB323717)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 26892079).
The expression of MARCO/Macrophage receptor is induced by Lipopolysaccharides (LPS) treatment (PMID : 9916718).
In Western blot loading control Anti-Vinculin antibody [EPR8185] (ab129002)(1/5000)(124KDa).
All lanes:
Western blot - Anti-MARCO antibody [RM1219] (ab323717) at 1/1000 dilution
Lane 1:
Untreated J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage ), whole cell lysate at 40 µg
Lane 2:
J774A.1 treated with 1ug/ml Lipopolysaccharide (LPS) and 300ng/ml 24h Brefeldin A (BFA) for 24 hours, whole cell lysate at 40 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 60-75 kDa,124 kDa
false
Exposure time: 180s
- WB
Supplier Data
Western blot - Anti-MARCO antibody [RM1219] (AB323717)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
Negative control : pancreas, skeletal muscle.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
All lanes:
Western blot - Anti-MARCO antibody [RM1219] (ab323717) at 1/1000 dilution
Lane 1:
Mouse lymph node tissue lysate at 20 µg
Lane 2:
Mouse pancreas tissue lysate at 20 µg
Lane 3:
Mouse skeletal muscle tissue lysate at 20 µg
Lane 4:
Rat lymph node tissue lysate at 5 µg
Lane 5:
Rat skeletal muscle tissue lysate at 5 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 75 kDa
false
Exposure time: 59s
Reactivity data
製品の詳細
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- - The sensitivity of polyclonal antibodies by recognising multiple epitopes
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
View our range of recombinant multiclonal antibodies.
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