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AB323717

Anti-MARCO antibody [RM1219]

Anti-MARCO antibody [RM1219]

  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • 20ul selling size
  • 詳細を見る

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Rabbit Recombinant Multiclonal MARCO antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.

別名を表示する

Macrophage receptor MARCO, Macrophage receptor with collagenous structure, Marco, SCARA2, MARCO, Macrophage receptor MARCO, Macrophage receptor with collagenous structure, Scavenger receptor class A member 2

22 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling MARCO with ab323717 at 1/200 (2.49 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on the Kupffer cells in human liver. The section was incubated with ab323717 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling MARCO with ab323717 at 1/200 (2.49 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining in human pancreas. The section was incubated with ab323717 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [RM1219] (AB323717)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [RM1219] (AB323717)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized Human PBMC (human primary peripheral blood mononuclear cell) cells labelling MARCO with ab323717 at 1/500 (0.996 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing the cytoplasmic and membranous staining is increased in subsets of human PBMCs (shown in green) treated with 50 ng/ml M-CSF for 72 hours, then add 20 ng/ml IL-10 for 24 hours. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)
  • mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human liver tissue staining MARCO with ab323717 at a 1 : 200 (2.49 ug/ml) dilution, ab213363 anti-CD68 used at 1 : 500 (1.26 ug/ml) dilution and ab237721 anti-CD3 used at a 1 : 2000 (0.26 ug/ml) dilution.

Panel A : merged staining of anti-MARCO (green; Opal™520), anti-CD68 (magenta; Opal™570) and anti-CD3 (yellow; Opal™690) on human liver.
Panel B : anti-MARCO staining macrophages in human liver.
Panel C : ant-CD68 staining macrophages in human liver.
Panel D : ant-CD3 staining T lymphocytes in human liver.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323717, ab213363 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling MARCO with ab323717 at 1/200 (2.49 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on the macrophages in human lung. The section was incubated with ab323717 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)
  • mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human liver tissue staining MARCO with ab323717 at a 1 : 200 (2.49 ug/ml) dilution, ab320735 anti-CD19 used at 1 : 2000 (0.255 ug/ml) dilution and ab237721 anti-CD3 used at a 1 : 2000 (0.26 ug/ml) dilution.

Panel A : merged staining of anti-MARCO (green; Opal™520), anti-CD19 (gray; Opal™570) and anti-CD3 (magenta; Opal™690) on human liver.
Panel B : anti-MARCO staining macrophages in human liver.
Panel C : ant-CD19 staining B lymphocytes in human liver.
Panel D : ant-CD3 staining T lymphocytes in human liver.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323717, ab320735 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)
  • mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse spleen tissue staining MARCO with ab323717 at a 1 : 200 (2.49 ug/ml) dilution, ab245235 anti-CD19 used at 1 : 1000 (0.22 ug/ml) dilution and ab237721 anti-CD3 used at a 1 : 2000 (0.26 ug/ml) dilution.

Panel A : merged staining of anti-MARCO (green; Opal™520), anti-CD19 (gray; Opal™690) and anti-CD3 (magenta; Opal™570) on mouse spleen.
Panel B : anti-MARCO staining macrophages in mouse spleen.
Panel C : ant-CD19 staining B lymphocytes in mouse spleen.
Panel D : ant-CD3 staining T lymphocytes in mouse spleen.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323717, ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)
  • mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse liver tissue staining MARCO with ab323717 at a 1 : 200 (2.49 ug/ml) dilution, ab245235 anti-CD19 used at 1 : 1000 (0.22 ug/ml) dilution and ab237721 anti-CD3 used at a 1 : 2000 (0.26 ug/ml) dilution.

Panel A : merged staining of anti-MARCO (green; Opal™520), anti-CD19 (gray; Opal™690) and anti-CD3 (magenta; Opal™570) on mouse liver.
Panel B : anti-MARCO staining macrophages in mouse liver.
Panel C : ant-CD19 staining B lymphocytes in mouse liver.
Panel D : ant-CD3 staining T lymphocytes in mouse liver.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323717, ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [RM1219] (AB323717)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MARCO antibody [RM1219] (AB323717)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized J774A.1(mouse reticulum cell sarcoma monocyte/macrophage) cells labelling MARCO with ab323717 at 1/500 (0.996 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing increased cytoplasmic staining in J774A.1 cells treated with 1 ng/ml lipopolysaccharide (LPS) for 24 hours (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling MARCO with ab323717 at 1/400 (1.245 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Positive staining on the Kupffer cells in rat liver. The primary antibody was incubated overnight at 4°C.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)

Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling MARCO with ab323717 at 1/400 (1.245 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Positive staining on the macrophages in rat lung. The primary antibody was incubated overnight at 4°C.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling MARCO with ab323717 at 1/200 (2.49 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on the Kupffer cells in mouse liver. The section was incubated with ab323717 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)
  • mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse lung tissue staining MARCO with ab323717 at a 1 : 200 (2.49 ug/ml) dilution, ab245235 anti-CD19 used at 1 : 1000 (0.22 ug/ml) dilution and ab237721 anti-CD3 used at a 1 : 2000 (0.26 ug/ml) dilution.

Panel A : merged staining of anti-MARCO (green; Opal™520), anti-CD19 (gray; Opal™690) and anti-CD3 (magenta; Opal™570) on mouse lung.
Panel B : anti-MARCO staining macrophages in mouse lung.
Panel C : ant-CD19 staining B lymphocytes in mouse lung.
Panel D : ant-CD3 staining T lymphocytes in mouse lung.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323717, ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling MARCO with ab323717 at 1/200 (2.49 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on the macrophages in mouse lung. The section was incubated with ab323717 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)
  • mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat liver tissue staining MARCO with ab323717 at a 1 : 200 (2.49 ug/ml) dilution, ab283654 anti-CD68 used at 1 : 100 (4.43 ug/ml) dilution and ab237721 anti-CD3 used at a 1 : 2000 (0.26 ug/ml) dilution.

Panel A : merged staining of anti-MARCO (green; Opal™520), anti-CD68 (magenta; Opal™690) and anti-CD3 (yellow; Opal™570) on rat liver.
Panel B : anti-MARCO staining macrophages in rat liver.
Panel C : ant-CD68 staining macrophages in rat liver.
Panel D : ant-CD3 staining T lymphocytes in rat liver.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323717, ab283654 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)
  • mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse lung tissue staining MARCO with ab323717 at a 1 : 200 (2.49 ug/ml) dilution, ab283654 anti-CD68 used at 1 : 100 (4.43 ug/ml) dilution and ab237721 anti-CD3 used at a 1 : 2000 (0.26 ug/ml) dilution.

Panel A : merged staining of anti-MARCO (green; Opal™520), anti-CD68 (magenta; Opal™690) and anti-CD3 (yellow; Opal™570) on mouse lung.
Panel B : anti-MARCO staining macrophages in mouse lung.
Panel C : ant-CD68 staining macrophages in mouse lung.
Panel D : ant-CD3 staining T lymphocytes in mouse lung.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323717, ab283654 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)

Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling MARCO with ab323717 at 1/200 (2.49 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining in mouse pancreas. The section was incubated with ab322705 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCO antibody [RM1219] (AB323717)

Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling MARCO with ab323717 at 1/400 (1.245 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Negative control : no staining in rat pancreas. The primary antibody was incubated overnight at 4°C.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)
  • mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-MARCO antibody [RM1219] (AB323717)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat liver tissue staining MARCO with ab323717 at a 1 : 200 (2.49 ug/ml) dilution, ab64088 anti-CD20 used at 1 : 100 (2.91 ug/ml) dilution and ab237721 anti-CD3 used at a 1 : 2000 (0.26 ug/ml) dilution.

Panel A : merged staining of anti-MARCO (green; Opal™520), anti-CD20 (gray; Opal™690) and anti-CD3 (magenta; Opal™570) on rat liver.
Panel B : anti-MARCO staining macrophages in rat liver.
Panel C : ant-CD20 staining B lymphocytes in rat liver.
Panel D : ant-CD3 staining T lymphocytes in rat liver.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323717, ab64088 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Western blot - Anti-MARCO antibody [RM1219] (AB323717)
  • WB

Supplier Data

Western blot - Anti-MARCO antibody [RM1219] (AB323717)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : pancreas.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MARCO antibody [RM1219] (ab323717) at 1/1000 dilution

Lane 1:

Human spleen tissue lysate at 40 µg

Lane 2:

Human pancreas tissue lysate at 40 µg

Lane 3:

Human lung tissue lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 75 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-MARCO antibody [RM1219] (AB323717)
  • WB

Supplier Data

Western blot - Anti-MARCO antibody [RM1219] (AB323717)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 26892079).

The expression of MARCO/Macrophage receptor is induced by Lipopolysaccharides (LPS) treatment (PMID : 9916718).

In Western blot loading control Anti-Vinculin antibody [EPR8185] (ab129002)(1/5000)(124KDa).

All lanes:

Western blot - Anti-MARCO antibody [RM1219] (ab323717) at 1/1000 dilution

Lane 1:

Untreated J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage ), whole cell lysate at 40 µg

Lane 2:

J774A.1 treated with 1ug/ml Lipopolysaccharide (LPS) and 300ng/ml 24h Brefeldin A (BFA) for 24 hours, whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 60-75 kDa,124 kDa

false

Exposure time: 180s

Western blot - Anti-MARCO antibody [RM1219] (AB323717)
  • WB

Supplier Data

Western blot - Anti-MARCO antibody [RM1219] (AB323717)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : pancreas, skeletal muscle.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MARCO antibody [RM1219] (ab323717) at 1/1000 dilution

Lane 1:

Mouse lymph node tissue lysate at 20 µg

Lane 2:

Mouse pancreas tissue lysate at 20 µg

Lane 3:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 4:

Rat lymph node tissue lysate at 5 µg

Lane 5:

Rat skeletal muscle tissue lysate at 5 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75 kDa

false

Exposure time: 59s

Key facts

宿主種

Rabbit

クローン性

Multiclonal

クローン番号

RM1219

アイソタイプ

IgG

キャリアフリー

No

交差種

Human, Mouse, Rat

アプリケーション

IHC-P, ICC/IF, WB

applications

免疫原

This product was produced with the following immunogens:

The exact immunogen used to generate this antibody is proprietary information.

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

ターゲットの情報

Pattern recognition receptor (PRR) which binds Gram-positive and Gram-negative bacteria (PubMed : 7867067). Also plays a role in binding of unopsonized particles by alveolar macrophages (By similarity). Binds to the secretoglobin SCGB3A2 (By similarity).
See full target information Marco

追加のターゲット

MARCO,

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