Anti-MARCKS (phospho S158) 抗体 [EP2113Y] (ab81295)
![Western blot - Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295)](https://www.abcam.co.jp/ps/products/81/ab81295/Images/ab81295-239710-anti-marcks-phospho-s158-antibody-ep2113y-western-blot.jpg "Western blot - Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2113Y] to MARCKS (phospho S158)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MARCKS (phospho S158) antibody [EP2113Y]
MARCKS 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2113Y] to MARCKS (phospho S158) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF or IHC-P -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Hela cell lysates treated with TPA.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2113Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81295の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 32 kDa. |
追加情報
Is unsuitable for ICC/IF or IHC-P.
ターゲット情報
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機能
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. -
配列類似性
Belongs to the MARCKS family. -
翻訳後修飾
Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity. -
細胞内局在
Cytoplasm > cytoskeleton. Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4082 Human
- Entrez Gene: 17118 Mouse
- Entrez Gene: 25603 Rat
- Omim: 177061 Human
- SwissProt: P29966 Human
- SwissProt: P26645 Mouse
- SwissProt: P30009 Rat
- Unigene: 519909 Human
see all -
別名
- 80 kDa protein antibody
- 80K L antibody
- 80K L protein antibody
see all
画像
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Western blot - Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295)All lanes : Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295) at 1/2000 dilution (purified)
Lane 1 : Rat brain
Lane 2 : Mouse brain
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 32 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295)](https://www.abcam.co.jp/ps/products/81/ab81295/Images/ab81295-239709-anti-marcks-phospho-s158-antibody-ep2113y-western-blot.jpg)
Western blot - Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295)All lanes : Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295) at 1/10000 dilution (purified)
Lane 1 : HEK293 cells treated with TPA
Lane 2 : HEK293 cells treated with TPA, and the PVDF membrane treated with lambda phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 32 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295)](https://www.abcam.co.jp/ps/products/81/ab81295/Images/ab81295-96500-anti-marcks-phospho-s158-antibody-ep2113y-western-blot.jpg)
Western blot - Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295)All lanes : Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295) at 1/2000 dilution (unpurified)
Lane 1 : Hela cell lysates, untreated.
Lane 2 : Hela cell lysates treated with TPA.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 32 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab81295 は 10 報の論文で使用されています。
- Zhong C et al. Protein Kinase C-Mediated Hyperphosphorylation and Lateralization of Connexin 43 Are Involved in Autoimmune Myocarditis-Induced Prolongation of QRS Complex. Front Physiol 13:815301 (2022). PubMed: 35418879
- Zhang J et al. Epithelial Gasdermin D shapes the host-microbial interface by driving mucus layer formation. Sci Immunol 7:eabk2092 (2022). PubMed: 35119941
- Wang CN et al. Targeting the phosphorylation site of myristoylated alanine-rich C kinase substrate alleviates symptoms in a murine model of steroid-resistant asthma. Br J Pharmacol 176:1122-1134 (2019). PubMed: 30706455
- Yang DC et al. Tackling MARCKS-PIP3 circuit attenuates fibroblast activation and fibrosis progression. FASEB J 33:14354-14369 (2019). PubMed: 31661644
- Rohrbach TD et al. MARCKS phosphorylation is modulated by a peptide mimetic of MARCKS effector domain leading to increased radiation sensitivity in lung cancer cell lines. Oncol Lett 13:1216-1222 (2017). WB ; Human . PubMed: 28454237
- Rohrbach TD et al. Targeting the effector domain of the myristoylated alanine rich C-kinase substrate enhances lung cancer radiation sensitivity. Int J Oncol 46:1079-88 (2015). PubMed: 25524703
- Jarboe JS et al. MARCKS regulates growth and radiation sensitivity and is a novel prognostic factor for glioma. Clin Cancer Res 18:3030-41 (2012). WB . PubMed: 22619307
- Rumsby M et al. Human prostate cell lines from normal and tumourigenic epithelia differ in the pattern and control of choline lipid headgroups released into the medium on stimulation of protein kinase C. Br J Cancer 104:673-84 (2011). WB ; Human . PubMed: 21266973
- Chen X & Rotenberg SA PhosphoMARCKS drives motility of mouse melanoma cells. Cell Signal 22:1097-103 (2010). PubMed: 20211725
- Gambaryan S et al. Thrombin and collagen induce a feedback inhibitory signaling pathway in platelets involving dissociation of the catalytic subunit of protein kinase A from an NFkappaB-IkappaB complex. J Biol Chem 285:18352-63 (2010). PubMed: 20356841