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リコンビナントRabMAb

Anti-MARCKS like protein 抗体 [EPR15353] - C-terminal (ab184546)

Submit a review Submit a question References (4)
Western blot - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)
  • Western blot - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)
  • Immunocytochemistry/ Immunofluorescence - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)
  • Flow Cytometry (Intracellular) - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)
  • Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15353] to MARCKS like protein - C-terminal
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-MARCKS like protein antibody [EPR15353] - C-terminal
    MARCKS like protein 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR15353] to MARCKS like protein - C-terminal

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • 293T, SH-SY5Y and Human fetal brain lysates; Human colon tissue; 293T and SH-SY5Y cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184546の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/140.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Detects a band of approximately 42-48 kDa (predicted molecular weight: 20 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/250.
特記事項
Flow Cyt (Intra)
1/140.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Detects a band of approximately 42-48 kDa (predicted molecular weight: 20 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/250.

ターゲット情報

画像

  • Western blot - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)
    Western blot - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)
    All lanes : Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546) at 1/10000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : SH-SY5Y cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 20 kDa



    The protein has a calculated molecular weight of 20 kDa and an apparent molecular weight of 42-48 kDa due to anomalous migration on SDS gels or phosphorylation

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)

    Immunohistochemical analysis of Paraffin-embedded Human colon tissue labeling MARCKS like protein with ab184546 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)
    Western blot - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)
    Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546) at 1/50000 dilution + Human fetal brain tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 20 kDa



    The protein has a calculated molecular weight of 20 kDa and an apparent molecular weight of 42-48 kDa due to anomalous migration on SDS gels or phosphorylation.

  • Immunocytochemistry/ Immunofluorescence - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)
    Immunocytochemistry/ Immunofluorescence - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)

    Immunofluorescent analysis of 293T cells (4% paraformaldehyde-fixed) labeling MARCKS like protein with ab184546 at 1/250 dilution (red) followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Flow Cytometry (Intracellular) - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)
    Flow Cytometry (Intracellular) - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)

    Intracellular flow cytometric analysis of SH-SY5Y cells (2% paraformaldehyde-fixed) labeling MARCKS like proteinwith ab184546 at 1/140 dilution (red)or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. 

     

     

  • Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)
    Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)

参考文献 (4)

ab184546 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab184546 は 4 報の論文で使用されています。

  • Zhang G  et al. A Novel Molecular Classification Method for Glioblastoma Based on Tumor Cell Differentiation Trajectories. Stem Cells Int 2023:2826815 (2023). PubMed: 37964983
  • Neumann EK  et al. Protocol for multimodal analysis of human kidney tissue by imaging mass spectrometry and CODEX multiplexed immunofluorescence. STAR Protoc 2:100747 (2021). PubMed: 34430920
  • Song C  et al. Myristoylated alanine-rich C kinase substrate-like protein-1 regulates epithelial sodium channel activity in renal distal convoluted tubule cells. Am J Physiol Cell Physiol 319:C589-C604 (2020). PubMed: 32639874
  • Kamal AHM  et al. Cross-linking Proteomics Indicates Effects of Simvastatin on the TLR2 Interactome and Reveals ACTR1A as a Novel Regulator of the TLR2 Signal Cascade. Mol Cell Proteomics 18:1732-1744 (2019). PubMed: 31221720

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