Anti-MAPRE1/EB1 抗体 (ab50188)
Key features and details
- Rabbit polyclonal to MAPRE1/EB1
- Suitable for: WB, IP, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-MAPRE1/EB1 antibody
MAPRE1/EB1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MAPRE1/EB1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken, Cow, Dog, Pig -
免疫原
Synthetic peptide corresponding to Human MAPRE1/EB1 aa 252-268 (C terminal) conjugated to keyhole limpet haemocyanin.
Sequence:GFVIPDEGGPQEEQEEY
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ポジティブ・コントロール
- Cell lysate of the rat fibroblast cell line Rat1, Rat1 cells and a HeLa cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab50188 is affinity-purified using the immunizing peptide immobilized on agarose. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50188の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 30 kDa.
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IP |
Use a concentration of 10 - 20 µg/ml.
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ICC/IF | (3) |
Use a concentration of 1 - 2 µg/ml.
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特記事項 |
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 30 kDa. |
IP
Use a concentration of 10 - 20 µg/ml. |
ICC/IF
Use a concentration of 1 - 2 µg/ml. |
ターゲット情報
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機能
May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration. -
組織特異性
Ubiquitously expressed. -
配列類似性
Belongs to the MAPRE family.
Contains 1 CH (calponin-homology) domain.
Contains 1 EB1 C-terminal domain. -
ドメイン
Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1. -
細胞内局在
Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Associated with the microtubule network at the growing distal tip of microtubules. Also enriched at the centrosome. - Information by UniProt
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参照データベース
- Entrez Gene: 419288 Chicken
- Entrez Gene: 507845 Cow
- Entrez Gene: 22919 Human
- Entrez Gene: 13589 Mouse
- Entrez Gene: 114764 Rat
- Omim: 603108 Human
- SwissProt: Q5ZLC7 Chicken
- SwissProt: Q3ZBD9 Cow
see all -
別名
- 5530600P05Rik antibody
- Adenomatous polyposis coli binding protein EB 1 antibody
- Adenomatous polyposis coli binding protein EB1 antibody
see all
画像
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ab50188 at 1ug/ml dilution staining the “plus-end tracking protein” (+TIP) structures in Rat1 fibroblasts grown to semi-confluency and fixed with acetone/methanol. The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugate. Antibody dilution 1:1,000.
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All lanes : Anti-MAPRE1/EB1 antibody (ab50188) at 0.5 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : NIH3T3 cell lysate
Lane 3 : Rat1 cell lysate
Lane 4 : A431 cell lysate
Lane 5 : SW48 cell lysate
Lane 6 : HCT116 cell lysate
Lane 7 : HEK293 cell lysate
Lane 8 : HeLa cell lysate with EB1 immunizing peptide at 10 µg/ml
Secondary
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate at 1/1000 dilution
Predicted band size: 30 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
50kDa band represents Rb IgG heavy chain -
RIPA lysate of HeLa cells immunoprecipitated with ab50188, separated SDS-PAGE and blotted with ab50188. 50kDa band represents Rb IgG heavy chain
Lane 1 : No primary antibody
Lanes 2-4 : Anti-MAPRE1/EB1 antibody (ab50188) at 15 µg
Lanes 1-2 : No lysate
Lane 3 : HeLa cell lysate at 100 µg
Lane 4 : HeLa cell lysate at 25 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate at 1/1000 dilution
Observed band size: 34 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab50188 は 7 報の論文で使用されています。
- Zhang L et al. Circular RNA SOX13 promotes malignant behavior and cisplatin resistance in non-small cell lung cancer through targeting microRNA-3194-3p/microtubule-associated protein RP/EB family member 1. Bioengineered 13:1814-1827 (2022). PubMed: 34709968
- Logan CM et al. Functional role for stable microtubules in lens fiber cell elongation. Exp Cell Res 362:477-488 (2018). PubMed: 29253534
- Almada E et al. Akap350 Recruits Eb1 to The Spindle Poles, Ensuring Proper Spindle Orientation and Lumen Formation in 3d Epithelial Cell Cultures. Sci Rep 7:14894 (2017). PubMed: 29097729
- Shimozawa T et al. Improving spinning disk confocal microscopy by preventing pinhole cross-talk for intravital imaging. Proc Natl Acad Sci U S A 110:3399-404 (2013). ICC/IF ; Human . PubMed: 23401517
- Goldspink DA et al. The microtubule end-binding protein EB2 is a central regulator of microtubule reorganisation in apico-basal epithelial differentiation. J Cell Sci 126:4000-14 (2013). PubMed: 23813963
- Nakamura S et al. Dissecting the nanoscale distributions and functions of microtubule-end-binding proteins EB1 and ch-TOG in interphase HeLa cells. PLoS One 7:e51442 (2012). WB ; Human . PubMed: 23251535
- Brüning-Richardson A et al. EB1 is required for spindle symmetry in mammalian mitosis. PLoS One 6:e28884 (2011). PubMed: 22216133