Anti-MAP4K6 抗体 - C-terminal (ab154256)
Key features and details
- Rabbit polyclonal to MAP4K6 - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MAP4K6 antibody - C-terminal -
製品の詳細
Rabbit polyclonal to MAP4K6 - C-terminal -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 901-1120 of Human MAP4K6 (Uniprot ID: Q8N4C8).
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ポジティブ･コントロール
- HepG2 whole cell lysate (ab7900); U87 xenograft; HeLa cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154256の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 150 kDa.
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| IHC-P |
1/100 - 1/1000.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 150 kDa. |
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IHC-P
1/100 - 1/1000. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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関連性
MAP4K6 is a serine/threonine kinase belonging to the germinal center kinase (GCK) family. These kinases regulate a wide variety of cellular processes by coupling cell surface receptors to MAPK pathways. This kinase appears to act upstream of the JUN N-terminal pathway and may be involved in brain development. It may also play a role in the response to environmental stress, in cytoskeleton reorganization, cell adhesion, and cell motility. Alternative splicing occurs at this locus and four transcript variants encoding distinct isoforms have been identified. -
細胞内局在
Cytoplasm. Cell junction; synapse; postsynaptic cell membrane; postsynaptic density. Cell projection; axon. Cell projection; dendrite. Isoform 4: Golgi apparatus -
参照データベース
- Entrez Gene: 50488 Human
- Omim: 609426 Human
- SwissProt: Q8N4C8 Human
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別名
- B55 antibody
- GCK family kinase MiNK antibody
- hMINK antibody
see all
画像
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Western blot - Anti-MAP4K6 antibody - C-terminal (ab154256)Anti-MAP4K6 antibody - C-terminal (ab154256) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 150 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4K6 antibody - C-terminal (ab154256)Immunohistochemical analysis of paraffin-embedded U87 xenograft tissue labeling MAP4K6 with ab154256 at 1/500 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-MAP4K6 antibody - C-terminal (ab154256)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling MAP4K6 with ab154256 at 1/200 dilution. Lower panel merged with DNA probe.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab154256 は 1 報の論文で使用されています。
- Zhang J et al. LncRNA SNHG14 contributes to proinflammatory cytokine production in rheumatoid arthritis via the regulation of the miR-17-5p/MINK1-JNK pathway. Environ Toxicol 36:2484-2492 (2021). PubMed: 34529319