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リコンビナントRabMAb

Anti-MAP4K4/NIK 抗体 [SP177] (ab150300)

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Flow Cytometry (Intracellular) - Anti-MAP4K4/NIK antibody [SP177] (ab150300)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4K4/NIK antibody [SP177] (ab150300)
  • Anti-MAP4K4/NIK antibody [SP177] (ab150300)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP177] to MAP4K4/NIK
  • Suitable for: Flow Cyt (Intra), IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-MAP4K4/NIK antibody [SP177]
    MAP4K4/NIK 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [SP177] to MAP4K4/NIK

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), IHC-Pmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse

  • 免疫原

    Synthetic peptide within Human MAP4K4/NIK aa 850-950 (C terminal). The exact sequence is proprietary.
    Database link: O95819

  • ポジティブ･コントロール

    • Human prostate adenocarcinoma tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • バッファー

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • 精製度

    Protein A/G purified

  • 特記事項（精製）

    Purified from TCS by protein A/G.

  • ポリ/モノ

    モノクローナル

  • クローン名

    SP177

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab150300の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/400.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
Flow Cyt (Intra)
1/400.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway.

  • 組織特異性

    Appears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.

  • 配列類似性

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CNH domain.
    Contains 1 protein kinase domain.

  • 翻訳後修飾

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • FLH21957 antibody
      • FLJ10410 antibody
      • FLJ20373 antibody
      • FLJ90111 antibody
      • Hepatocyte progenitor kinase like/germinal center kinase like kinase antibody
      • HGK antibody
      • HPK/GCK like kinase antibody
      • HPK/GCK like kinase HGK antibody
      • HPK/GCK-like kinase HGK antibody
      • KIAA0687 antibody
      • M4K4_HUMAN antibody
      • MAP4K4 antibody
      • MAPK/ERK kinase kinase kinase 4 antibody
      • MEK kinase kinase 4 antibody
      • MEKKK 4 antibody
      • MEKKK4 antibody
      • Mitogen activated protein kinase kinase kinase kinase 4 antibody
      • Mitogen-activated protein kinase kinase kinase kinase 4 antibody
      • Nck interacting kinase antibody
      • Nck-interacting kinase antibody
      • NIK antibody
      see all

    画像

    • Flow Cytometry (Intracellular) - Anti-MAP4K4/NIK antibody [SP177] (ab150300)
      Flow Cytometry (Intracellular) - Anti-MAP4K4/NIK antibody [SP177] (ab150300)

      Flow cytometric analysis of rabbit anti-MAP4K4/NIK (SP177) antibody ab150300 (1/400) in HepG2 cells(green) compared to negative control of rabbit IgG (blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4K4/NIK antibody [SP177] (ab150300)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4K4/NIK antibody [SP177] (ab150300)

      Immunohistochemical analysis of formalin fixed, paraffin embedded Human prostate adenocarcinoma tissue labelling MAP4K4/NIK with ab150300 at 1/100 dilution.

    • Anti-MAP4K4/NIK antibody [SP177] (ab150300)
      Anti-MAP4K4/NIK antibody [SP177] (ab150300)

    参考文献 (0)

    ab150300 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab150300 は論文での使用が確認できていません。

    レビューと Q&A

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    There are currently no Customer reviews or Questions for ab150300.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com