AB155583

Anti-MAP4K4/NIK 抗体

Anti-MAP4K4/NIK antibody

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(10 Publications)

Rabbit Polyclonal MAP4K4/NIK antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human MAP4K4 aa 350-450.

別名を表示する

HGK, KIAA0687, NIK, MAP4K4, Mitogen-activated protein kinase kinase kinase kinase 4, HPK/GCK-like kinase HGK, MAPK/ERK kinase kinase kinase 4, Nck-interacting kinase, MEK kinase kinase 4, MEKKK 4

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-MAP4K4/NIK antibody (AB155583)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MAP4K4/NIK antibody (AB155583)

Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling MAP4K4/NIK with ab155583 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4K4/NIK antibody (AB155583)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4K4/NIK antibody (AB155583)

Immunohistochemical analysis of paraffin-embedded Human SNU1 xenograft tissue labeling MAP4K4/NIK with ab155583 at 1/250 dilution.

Western blot - Anti-MAP4K4/NIK antibody (AB155583)

WB

Unknown

Western blot - Anti-MAP4K4/NIK antibody (AB155583)

5% SDS PAGE

All lanes:

Western blot - Anti-MAP4K4/NIK antibody (ab155583) at 1/1000 dilution

All lanes:

Raji whole cell lysate at 30 µg

Predicted band size: 142 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

ICC/IF, WB, IHC-P

免疫原

Synthetic Peptide within Human MAP4K4 aa 350-450. The exact immunogen used to generate this antibody is proprietary information.

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代替製品

Primary Antibodies

AB150300

Anti-MAP4K4/NIK antibody [SP177]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-MAP4K4/NIK antibody [SP177] (AB150300)

0
0

Primary Antibodies

AB245752

Anti-MAP4K4/NIK antibody [SP177] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-MAP4K4/NIK antibody [SP177] - BSA and Azide free (AB245752)

0
0

Primary Antibodies

AB243321

Anti-MAP4K4/NIK antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4K4/NIK antibody (AB243321)

0
0

Primary Antibodies

AB69885

Anti-MAP4K4/NIK antibody

Western blot - Anti-MAP4K4/NIK antibody (AB69885)

0
0

Primary Antibodies

AB80418

Anti-MAP4K4/NIK antibody

Immunoprecipitation - Anti-MAP4K4/NIK antibody (AB80418)

0
0

組合せ製品

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AB32537

Anti-ERK1 antibody [Y72]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Flow Cytometry (Intracellular) - Anti-ERK1 antibody [Y72] (AB32537)

5
4

Primary Antibodies

AB40766

Anti-c-Jun antibody [EP693Y]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [EP693Y] (AB40766)

5
2

Primary Antibodies

AB239357

Anti-TRAF3 antibody [EPR22992-93]

RabMAb|Recombinant|KO Validated

Western blot - Anti-TRAF3 antibody [EPR22992-93] (AB239357)

0
0

Primary Antibodies

AB32536

Anti-NF-kB p65 antibody [E379]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 antibody [E379] (AB32536)

4
15

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }

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製品の詳細

Keep as a concentrated solution.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Serine/threonine kinase that plays a role in the response to environmental stress and cytokines such as TNF. Appears to act upstream of the JUN N-terminal pathway (PubMed : 9890973). Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway (PubMed : 26437443). Phosphorylates SMAD1 on Thr-322 (PubMed : 21690388).

See full target information MAP4K4

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文献 (10)

Recent publications for all applications. Explore the full list and refine your search

Neoplasia (New York, N.Y.) 60:101102 PubMed39674114

2024

USP20 mediates malignant phenotypic changes in bladder cancer through direct interactions with YAP1.

Applications

Unspecified application

Species

Unspecified reactive species

Wensun Chen,Siqi Wu,Yifan Chen,Weijian Li,Yiqing Cao,Yingchun Liang,Xiyu Dai,Xinan Chen,Yilin Chen,Tian Chen,Shenghua Liu,Chen Yang,Haowen Jiang

Genes 13: PubMed36292671

2022

Bioinformatics and Experimental Analyses Reveal MAP4K4 as a Potential Marker for Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Junping Zhang,Xiaoping Cai,Weifeng Cui,Zheng Wei

International journal of molecular medicine 45:743-752 PubMed31922225

2020

Silencing CDC25A inhibits the proliferation of liver cancer cells by downregulating IL‑6 in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Si Chen,Yanping Tang,Chun Yang,Kezhi Li,Xiaoqing Huang,Ji Cao

Journal of natural medicines 73:777-788 PubMed31243669

2019

Sanguinarine inhibits the proliferation of BGC-823 gastric cancer cells via regulating miR-96-5p/miR-29c-3p and the MAPK/JNK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xian-Zhe Dong,Yan Song,Yu-Pan Lu,Yuan Hu,Ping Liu,Lan Zhang

Oncotarget 10:2644-2656 PubMed31080555

2019

MicroRNA profiling of pancreatic ductal adenocarcinoma (PDAC) reveals signature expression related to lymph node metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Moran Lemberger,Shelly Loewenstein,Nir Lubezky,Eran Nizri,Metsada Pasmanik-Chor,Eli Barazovsky,Joseph M Klausner,Guy Lahat

Bioscience reports 38: PubMed30429233

2018

Chemotherapy-induced /MAP4K4 signaling suppresses progression of colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Feifei Wang,Lianmei Zhao,Juan Zhang,Zesong Meng,Chaoxi Zhou,Guanglin Wang,Youqiang Liu,Meng Li,Jinchuan Xi,Wenbo Niu,Guiying Wang

PloS one 13:e0201321 PubMed30048526

2018

Identification of lead anti-human cytomegalovirus compounds targeting MAP4K4 via machine learning analysis of kinase inhibitor screening data.

Applications

Unspecified application

Species

Unspecified reactive species

Blair L Strang,Christopher R M Asquith,Hanan F Moshrif,Catherine M-K Ho,William J Zuercher,Hassan Al-Ali

Journal of neuropathology and experimental neurology 77:21-39 PubMed29186589

2017

Detergent Insoluble Proteins and Inclusion Body-Like Structures Immunoreactive for PRKDC/DNA-PK/DNA-PKcs, FTL, NNT, and AIFM1 in the Amygdala of Cognitively Impaired Elderly Persons.

Applications

Unspecified application

Species

Unspecified reactive species

Jozsef Gal,Jing Chen,Yuriko Katsumata,David W Fardo,Wang-Xia Wang,Sergey Artiushin,Douglas Price,Sonya Anderson,Ela Patel,Haining Zhu,Peter T Nelson

Experimental and therapeutic medicine 12:3405-3410 PubMed27882171

2016

Role of microRNA-141 in colorectal cancer with lymph node metastasis.

Applications

WB

Species

Human

Li Feng,Hongqing Ma,Liang Chang,Xinliang Zhou,Na Wang,Lianmei Zhao,Jing Zuo,Yudong Wang,Jing Han,Guiying Wang

Molecular medicine reports 13:41-8 PubMed26549737

2015

Silencing of MAP4K4 by short hairpin RNA suppresses proliferation, induces G1 cell cycle arrest and induces apoptosis in gastric cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan-Fei Liu,Guo-Qiang Qu,Yun-Min Lu,Wu-Ming Kong,Yuan Liu,Wei-Xiong Chen,Xiao-Hong Liao

View all publications

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Abcam product promise

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