Anti-MAP4 抗体 (ab245578)
Key features and details
- Rabbit polyclonal to MAP4
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MAP4 antibody
MAP4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MAP4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide within Human MAP4 aa 1102-1152. The exact sequence is proprietary. NP_002366.2
Database link: P27816 -
ポジティブ･コントロール
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human ovarian carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab245578 was affinity purified using an epitope specific to MAP4 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245578の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 121 kDa.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 121 kDa. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Non-neuronal microtubule-associated protein. Promotes microtubule assembly. -
配列類似性
Contains 4 Tau/MAP repeats. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylation on Ser-787 negatively regulates MAP4 activity to promote microtubule assembly. Isoform 3 is phosphorylated on Ser-337 and Ser-338. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 4134 Human
- Entrez Gene: 17758 Mouse
- Entrez Gene: 367171 Rat
- Omim: 157132 Human
- SwissProt: P27816 Human
- SwissProt: P27546 Mouse
- SwissProt: Q5M7W5 Rat
- Unigene: 517949 Human
see all -
別名
- DKFZp779A1753 antibody
- MAP-4 antibody
- MAP4 antibody
see all
画像
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Western blot - Anti-MAP4 antibody (ab245578)All lanes : Anti-MAP4 antibody (ab245578) at 0.04 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 121 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-MAP4 antibody (ab245578)MAP4 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab245578 used for IP at 3 µg/mg lysate. For WB 0.01 µg/ml.
Lane 1: ab245578 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.Chemiluminescence detection: 30 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4 antibody (ab245578)Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for MAP4 with ab245578 at a 1/200 dilution in immunohistochemical analysis. DAB staining.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab245578 は論文での使用が確認できていません。