AB245578

Anti-MAP4 抗体

Name: Anti-MAP4 抗体 (ab245578) | Abcam
Brand: Abcam Limited
SKU: AB245578
Price: 75000 JPY
Availability: InStock

Anti-MAP4 antibody

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(3 Publications)

Rabbit Polyclonal MAP4 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MAP4 aa 1100 to C-terminus.

別名を表示する

Microtubule-associated protein 4, MAP-4, MAP4

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4 antibody (AB245578)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4 antibody (AB245578)

Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for MAP4 with ab245578 at a 1/200 dilution in immunohistochemical analysis. DAB staining.

Supplier Data

Immunoprecipitation - Anti-MAP4 antibody (AB245578)

MAP4 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).

ab245578 used for IP at 3 μg/mg lysate. For WB 0.01 μg/ml.

Lane 1 : ab245578 IP in HeLa whole cell lysate.
Lane 2 : Control IgG in HeLa whole cell lysate.

Chemiluminescence detection : 30 seconds.

All lanes:

Immunoprecipitation - Anti-MAP4 antibody (ab245578)

Predicted band size: 121 kDa

false

Supplier Data

Western blot - Anti-MAP4 antibody (AB245578)

All lanes:

Western blot - Anti-MAP4 antibody (ab245578) at 0.04 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 121 kDa

false

Exposure time: 30s

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P, WB, IP

applications

免疫原

Synthetic Peptide within Human MAP4 aa 1100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P27816

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

ab245578 was affinity purified using an epitope specific to MAP4 immobilized on solid support.

バッファー組成

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Non-neuronal microtubule-associated protein. Promotes microtubule assembly.

See full target information MAP4

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:6322 PubMed37813836

2023

Polarized microtubule remodeling transforms the morphology of reactive microglia and drives cytokine release.

Applications

Unspecified application

Species

Unspecified reactive species

Max Adrian,Martin Weber,Ming-Chi Tsai,Caspar Glock,Olga I Kahn,Lilian Phu,Tommy K Cheung,William J Meilandt,Christopher M Rose,Casper C Hoogenraad

International journal of molecular sciences 24: PubMed36835542

2023

miR-103-3p Regulates the Differentiation and Autophagy of Myoblasts by Targeting MAP4.

Applications

Unspecified application

Species

Unspecified reactive species

Xianxian Zhang,Shihui Huang,Xi Niu,Sheng Li,Jiafu Wang,Xueqin Ran

Nature 594:560-565 PubMed34040253

2021

MARK4 controls ischaemic heart failure through microtubule detyrosination.

Applications

Unspecified application

Species

Unspecified reactive species

Xian Yu,Xiao Chen,Mamta Amrute-Nayak,Edward Allgeyer,Aite Zhao,Hannah Chenoweth,Marc Clement,James Harrison,Christian Doreth,George Sirinakis,Thomas Krieg,Huiyu Zhou,Hongda Huang,Kiyotaka Tokuraku,Daniel St Johnston,Ziad Mallat,Xuan Li

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MAP4 抗体

Anti-MAP4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4 antibody (AB245578)

Immunoprecipitation - Anti-MAP4 antibody (AB245578)

Western blot - Anti-MAP4 antibody (AB245578)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (3)

代替製品

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise