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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-MAP2K1IP1 抗体 [Y130] (ab32134)

Submit a review Submit a question References (4)
Western blot - Anti-MAP2K1IP1 antibody [Y130] (ab32134)
  • Western blot - Anti-MAP2K1IP1 antibody [Y130] (ab32134)
  • Immunocytochemistry/ Immunofluorescence - Anti-MAP2K1IP1 antibody [Y130] (ab32134)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2K1IP1 antibody [Y130] (ab32134)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2K1IP1 antibody [Y130] (ab32134)
  • Anti-MAP2K1IP1 antibody [Y130] (ab32134)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y130] to MAP2K1IP1
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-MAP2K1IP1 antibody [Y130]
    MAP2K1IP1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [Y130] to MAP2K1IP1

  • 由来種

    Rabbit

  • 特異性

    ab32134 recognises MP1 (MEK partner-1).

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa and A431 cell lysates. IHC-P: Human breast carcinoma tissue. ICC/IF: MCF7 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    Y130

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab32134の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/10000 - 1/50000. Detects a band of approximately 14 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100.
特記事項
WB
1/10000 - 1/50000. Detects a band of approximately 14 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100.
追加情報
Is unsuitable for Flow Cyt.

ターゲット情報

  • 機能

    As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2.

  • 配列類似性

    Belongs to the LAMTOR3 family.

  • 細胞内局在

    Late endosome membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • LAMTOR3 antibody
      • Late endosomal/lysosomal adaptor and MAPK and MTOR activator 3 antibody
      • LTOR3_HUMAN antibody
      • MAP2K1IP1 antibody
      • MEK binding partner 1 antibody
      • MEK partner 1 antibody
      • MEK-binding partner 1 antibody
      • Mitogen activated protein kinase kinase 1 interacting protein 1 antibody
      • Mitogen-activated protein kinase kinase 1-interacting protein 1 antibody
      • Mitogen-activated protein kinase scaffold protein 1 antibody
      • Mp1 antibody
      • Ragulator complex protein LAMTOR3 antibody
      see all

    画像

    • Western blot - Anti-MAP2K1IP1 antibody [Y130] (ab32134)
      Western blot - Anti-MAP2K1IP1 antibody [Y130] (ab32134)
      All lanes : Anti-MAP2K1IP1 antibody [Y130] (ab32134) at 1/50000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : MAP2K1IP1 knockout HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.


      Lanes 1-2: Merged signal (red and green). Green - ab32134 observed at 14 kDa. Red - loading control ab8245 observed at 37 kDa.

       ab32134 Anti-MAP2K1IP1 antibody [Y130] was shown to specifically react with MAP2K1IP1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265624 (knockout cell lysate ab258021) was used. Wild-type and MAP2K1IP1 knockout samples were subjected to SDS-PAGE. ab32134 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 50000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

       

    • Western blot - Anti-MAP2K1IP1 antibody [Y130] (ab32134)
      Western blot - Anti-MAP2K1IP1 antibody [Y130] (ab32134)
      Anti-MAP2K1IP1 antibody [Y130] (ab32134) at 1/50000 dilution + A431 cell lysate

      Observed band size: 14 kDa why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-MAP2K1IP1 antibody [Y130] (ab32134)
      Immunocytochemistry/ Immunofluorescence - Anti-MAP2K1IP1 antibody [Y130] (ab32134)

      Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling MAP2K1IP1 with purified ab32134 at 1/100 dilution (10 µg/mL). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2K1IP1 antibody [Y130] (ab32134)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2K1IP1 antibody [Y130] (ab32134)

      ab32134, staining human breast carcinoma by immunohistochemistry, Paraffin embedded tissue

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2K1IP1 antibody [Y130] (ab32134)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2K1IP1 antibody [Y130] (ab32134)

      ab32134 showing positive staining in Breast carcinoma tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-MAP2K1IP1 antibody [Y130] (ab32134)
      Anti-MAP2K1IP1 antibody [Y130] (ab32134)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (4)

    ab32134 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab32134 は 4 報の論文で使用されています。

    • Jin Z  et al. miR-330-3p suppresses liver cancer cell migration by targeting MAP2K1. Oncol Lett 18:314-320 (2019). PubMed: 31289502
    • Liu X  et al. Napabucasin Attenuates Resistance of Breast Cancer Cells to Tamoxifen by Reducing Stem Cell-Like Properties. Med Sci Monit 25:8905-8912 (2019). PubMed: 31760402
    • Schiefermeier N  et al. The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration. J Cell Biol 205:525-540 (2014). IP ; Human . PubMed: 24841562
    • Westerman BA  et al. A genome-wide RNAi screen in mouse embryonic stem cells identifies Mp1 as a key mediator of differentiation. J Exp Med 208:2675-89 (2011). PubMed: 22143885

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