Anti-MAP2 抗体

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody - Neuronal Marker (AB75713)

Immunocytochemistry/Immunofluorescence analysis of cultured neonatal mouse cortex cells labelling MAP2 (green) with ab75713. Cells were fixed with 4% paraformaldehyde. Red - rabbit anti-GFAP, Blue - DAPI nuclear stain.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody - Neuronal Marker (AB75713)

Immunocytochemistry/Immunofluorescence analysis of cultured neonatal mouse cortex cells labelling MAP2 (green) with ab75713. Cells were fixed with 4% paraformaldehyde. Red - rabbit anti-GFAP.

• ICC/IF

CiteAb

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody - Neuronal Marker (AB75713)

Immunocytochemistry-immunofluorescence using Anti-MAP2 antibody, ab75713. Publication image from Brouwer, M. et al., 2019, EMBO J, 31368584. Legend direct from paper.

SALM1 is localized to pre‐ and postsynapses of mouse excitatory hippocampal neurons (related to Appendix Figs S1–S3)Sandwich‐cultured mouse hippocampal neurons stained at DIV16 for endogenous SALM1 (green), dendritic marker MAP2 (blue), and synapse markers Homer or VGluT1 (red), or the axonal marker SMI‐312 (red). Boxes indicate area of zoom. Arrows indicate overlap between SALM1 and synapse markers. Bars = 10 µm in full neuron images. Bars = 5 µm in zoomed images.Average overlap ± SEM of SALM1 clusters with VGluT1 or Homer puncta. Numbers in bars indicate number of zoomed images/total number of neurons in two independent experiments.Example images showing differential overlap of SALM1 with Homer or VGluT1. Bars = 0.5 µm.Electron micrographs showing subsynaptic localization of endogenous SALM1 in mouse hippocampal brain slices. SALM1 immunogold particles are detected in presynapses (orange and magenta arrows) and postsynapses (cyan and blue arrows). Bars = 100 nm.Mean percentage of gold particles ± SEM detected in pre‐ versus postsynapses of mouse hippocampal slices stained for SALM1. Percentages are based on detected gold particles in 32 synapses in hippocampal brain slices of three different animals (Mann–Whitney U‐tests with Bonferroni correction, ns = not significant, *P < 0.025 after Bonferroni correction).Source data are available online for this figure.