Anti-MAP2 抗体 - Neuronal Marker

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody - Neuronal Marker (AB32454)

ab32454 staining MAP2 in Rat Primary Neurons DIV14 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab32454 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 4% paraformaldehyde (10 min).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody - Neuronal Marker (AB32454)

IHC image of MAP2 staining in human cerebral cortex FFPE section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32454, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• ICC/IF

AbReview47142****

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody - Neuronal Marker (AB32454)

ab32454 staining MAP2 in rat cryopreserved embryonic cortical neurons cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde with picric acid. Samples were incubated with primary antibody (1/2000 in 10mM PBS + 0.3% Triton-X) for 12 hours at 4°C. An Alexa Fluor^® 488-conjugated donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Babben Tinner

• IHC-P

AbReview22894****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody - Neuronal Marker (AB32454)

ab32454 staining MAP2 in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/3000 in blocking buffer) for 2 hours at 21°C in TBS/BSA/azide. An undiluted Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

This image is courtesy of an Abreview by Carl Hobbs.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody - Neuronal Marker (AB32454)

Rat astrocytes (DIV 22) cells were fixed with 4% paraformaldehyde and stained for MAP2 (Green) using ab32454 at 1/1000 dilution in ICC/IF analysis.

Müller A et al. Monitoring Astrocytic Proteome Dynamics by Cell Type-Specific Protein Labeling. PLoS One 10:e0145451 (2015).

• WB

PubMed

Western blot - Anti-MAP2 antibody - Neuronal Marker (AB32454)

All lanes:

Western blot - Anti-MAP2 antibody - Neuronal Marker (ab32454) at 1/1000 dilution

Lanes 1 - 2:

Trisomic induced Pluripotent Stem Cells (iPSCs)

Lanes 3 - 4:

Disomic induced Pluripotent Stem Cells (iPSCs)

Predicted band size: 199 kDa

false

Gonzales PK et al. Transcriptome analysis of genetically matched human induced pluripotent stem cells disomic or trisomic for chromosome 21. PLoS One 13:e0194581 (2018).

• WB

Lab

Western blot - Anti-MAP2 antibody - Neuronal Marker (AB32454)

Blocking buffer : 2% BSA

Gel type : TA

All lanes:

Western blot - Anti-MAP2 antibody - Neuronal Marker (ab32454) at 1 µg/mL

Lane 1:

Mouse Brain (day 0) Tissue Lysate at 20 µg

Lane 2:

Mouse Brain Tissue Lysate at 20 µg

Lane 3:

Rat Brain Tissue Lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 199 kDa

Observed band size: 268 kDa,280 kDa

false

Exposure time: 2min