Anti-MAP2 抗体 [EPR19691]

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Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB183830)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cells labelling MAP2 with ab183830 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing positive staining in rat primary neuron cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 µg/mL) (Red). The Nuclear counterstain was DAPI (Blue).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB183830)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling MAP2 with ab183830 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on neurons of human cerebral cortex is observed [PMID 15233758, PMID 19136970]. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB183830)

Immunohistochemical analysis of paraffin-embedded human Alzheimer's brain tissue* labelling MAP2 with ab183830 at 0.1ug/ml followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Positive staining in human Alzheimer's brain.

The section was incubated with ab183830 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0 epitope retrieval solution1) for 20 mins.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB183830)

Composite multiplex immunofluorescence staining of Iba1, GFAP and MAP2 staining in a section of formalin-fixed paraffin-embedded human cerebral cortex*.

Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate (Ph6.0) using retrieval settings of 100°C for 20 minutes. The section was then incubated at room temperature for 1 hour with ab300156 at 1µg/ml dilution (shown in green), ab183830 at 1µg/ml (shown in magenta), and ab302644 at 1µg/ml (shown in yellow). Then incubated for 1 hour with ab150161 Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed 1/1000, ab150083 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed 1/1000, and ab150134 Donkey Anti-Goat IgG H&L (Alexa Fluor® 555) preadsorbed 1/1000. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium ^®.

Image was taken with the EVOS™ S1000 Spatial Imaging System (ThermoFisher Scientific) with spectral unmixing and minor subsequent contrast adjustment.

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB183830)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling MAP2 with ab183830 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on human pancreas islet is observed [PMID : 9341200]. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB183830)

Immunofluorescence staining of MAP2 using ab183830 in ioGlutamatergic Neurons (Human iPSC-Derived Glutamatergic Neurons, ab259259), which were differentiated for 11 days post induction.

The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab183830 at 1 μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin, at 1/1000 dilution. Cells were then incubated with ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Images were acquired with the Perkin Elmer Operetta HCA and a maximum intensity projection of confocal sections is shown.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB183830)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MAP2 with ab183830 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human tonsil. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB183830)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural mix culture cells labelling Map2 with ab183830 at 1/500 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB183830)

Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling MAP2 with ab183830 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on myenteric nerve plexus of mouse stomach is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB183830)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Glutamate Receptor 1 (AMPA subtype) with ab174785 at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) at 1/1000 dilution at RT for 45 min. Anti-MAP2 antibody [EPR19691] (ab183830) was used as a counterstain at 1/1000 dilution and was co-incubated with ab174785 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB183830)

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling MAP2 with ab183830 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on neurons of mouse cerebral cortex is observed [PMID 15233758, PMID 19136970]. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB183830)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling MAP2 with ab183830 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on rat kidney is observed. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB183830)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse Cortex tissue labeling MAP2 with ab183830 at 1/100 dilution, followed by Goat anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on neurons of mouse Cortex is observed. The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 secondary antibody at 1/1000 dilution.

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Flow Cytometry (Intracellular) - Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB183830)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse brain cells cells labelling MAP2 with ab183830 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

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Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB183830)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neurons labelling Synaptophysin with ab309493 at 1/100 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in rat primary neuron cell line. Confocal scanning Z step was set as 0.3 µM followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab183830 Anti-MAP2 rabbit monoclonal antibody was used to counterstain tubulin at 1/1000 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

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Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB183830)

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Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB183830)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labelling Myelin Basic Protein with ab7349 at a 1/200 dilution, followed by Goat anti-Rat (Alexa Fluor^® 488) (ab150157) secondary antibody at a 1/1000 dilution. Confocal image showing positive staining in mouse primary oligodendroglia cells (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).

Counterstained with Anti-MAP2 antibody (ab183830) at a 1/1000 dilution, followed by Goat Anti-Rabbit secondary (Alexa Fluor^® 594) (ab150080) at a 1/1000 dilution (shown in magenta).

Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

The negative controls are as follows :

-ve control 1 : Myelin Basic Protein (ab7349) at a 1/200 dilution, followed by Goat anti-Rabbit (Alexa Fluor^® 594) (ab150080) at a 1/1000 dilution.

-ve control 2 : Anti-MAP2 (ab183830) at a 1/1000 dilution, followed by Goat anti-Rat (Alexa Fluor^® 488) (ab150157) at a 1/1000 dilution.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB183830)

Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling MAP2 with ab183830 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on neurons of rat hippocampus was observed [PMID 15233758, PMID 19136970]. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB183830)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat primary neuron cells labelling Glutamate Receptor 1 (AMPA subtype) with ab174785 at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) at 1/1000 dilution at RT for 45 min. Anti-MAP2 antibody [EPR19691] (ab183830) was used as a counterstain at 1/1000 dilution and was co-incubated with ab174785 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.

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Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB183830)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labeling MAP2 with ab300645 at 1/100 dilution followed by ab98736 Goat F(ab')2 Anti-Mouse IgG - Fc (DyLight® 488) pre-adsorbed at 1/1000 dilution (green). Counterstain was ab183830 Anti-MAP2 rabbit monoclonal antibody at 1/1000 dilution along with ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) as the counterstain secondary used at 1/1000 dilution. Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. The nuclear counter stain is DAPI (blue). Negative control 1 : ab300645 at 1/100 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 dilution. Negative control 2 : ab183830 at 1/1000 dilution followed by ab98736 Goat F(ab')2 Anti-Mouse IgG - Fc (DyLight® 488) pre-adsorbed at 1/1000 dilution.

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Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB183830)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized rat primary neural/glia cells labeling MAP2 with ab300645 at 1/100 dilution followed by ab98736 Goat F(ab')2 Anti-Mouse IgG - Fc (DyLight® 488) pre-adsorbed at 1/1000 dilution (green). Counterstain was ab183830 Anti-MAP2 rabbit monoclonal antibody at 1/1000 dilution along with ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) as the counterstain secondary used at 1/1000 dilution. Confocal image showing cytoplasmic staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. The nuclear counter stain is DAPI (blue).  Negative control 1 : ab300645 at 1/100 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 dilution. Negative control 2 : ab183830 at 1/1000 dilution followed by ab98736 Goat F(ab')2 Anti-Mouse IgG - Fc (DyLight® 488) pre-adsorbed at 1/1000 dilution.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB183830)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling MAP2 with ab183830 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on mouse kidney. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB183830)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling MAP2 with ab183830 at 1/100 dilution, followed by Goat anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Negative staining on mouse kidney. The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 secondary antibody at 1/1000 dilution.

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Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] - Neuronal Marker (AB183830)

MAP2 antibody ab183830 was used with 3D Cell Culture Clearing Kit ab243299 to penetrate, stain and clear a 3D neuronal spheroid cell culture. Blue : DAPI, Green : MAP2. Learn more about 3D cell culture and tissue clearing kits, reagents, and protocols designed to make it easier to stain 3D cell cultures and thick tissue sections and get more data from each valuable tissue section. To use this antibody with clearing, use 3D Cell Culture Clearing Kit ab243299. We recommend a starting dilution of 1 : 150, and also using Goat Anti-Rabbit IgG H&L AlexaFluor488 (ab150077) at a dilution of 1 : 400.