Anti-MAP2 抗体 [EPR19691] - Mouse IgG2a (Chimeric) (ab300645)

Immunohistochemical analysis of paraffin-embedded Human cerebrum labeling MAP2 with ab300645 at 1/10000 dilution (0.086 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on Human cerebrum. The section was incubated with ab300645 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Immunohistochemical analysis of paraffin-embedded Human colon labeling MAP2 with ab300645 at 1/10000 dilution (0.086 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on ganglion in the human colon. The section was incubated with ab300645 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Immunohistochemical analysis of paraffin-embedded Human pancreas labeling MAP2 with ab300645 at 1/10000 dilution (0.086 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on islet in the human pancreas. The section was incubated with ab300645 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum labeling MAP2 with ab300645 at 1/10000 dilution (0.086 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on Mouse cerebrum. The section was incubated with ab300645 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Immunohistochemical analysis of paraffin-embedded Mouse colon labeling MAP2 with ab300645 at 1/10000 dilution (0.086 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on ganglion in the mouse colon. The section was incubated with ab300645 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Immunohistochemical analysis of paraffin-embedded Rat cerebrum labeling MAP2 with ab300645 at 1/10000 dilution (0.086 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on Rat cerebrum. The section was incubated with ab300645 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Immunohistochemical analysis of paraffin-embedded Rat colon labeling MAP2 with ab300645 at 1/10000 dilution (0.086 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Posiive staining on ganglion in the rat colon. The section was incubated with ab300645 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labeling MAP2 with ab300645 at 1/100 dilution followed by ab98736 Goat F(ab')2 Anti-Mouse IgG - Fc (DyLight^® 488) pre-adsorbed at 1/1000 dilution (green). Counterstain was ab183830 Anti-MAP2 rabbit monoclonal antibody at 1/1000 dilution along with ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) as the counterstain secondary used at 1/1000 dilution. Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. The nuclear counter stain is DAPI (blue).

Negative control 1: ab300645 at 1/100 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at 1/1000 dilution.

Negative control 2: ab183830 at 1/1000 dilution followed by ab98736 Goat F(ab')2 Anti-Mouse IgG - Fc (DyLight^® 488) pre-adsorbed at 1/1000 dilution.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized rat primary neural/glia cells labeling MAP2 with ab300645 at 1/100 dilution followed by ab98736 Goat F(ab')2 Anti-Mouse IgG - Fc (DyLight^® 488) pre-adsorbed at 1/1000 dilution (green). Counterstain was ab183830 Anti-MAP2 rabbit monoclonal antibody at 1/1000 dilution along with ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) as the counterstain secondary used at 1/1000 dilution. Confocal image showing cytoplasmic staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. The nuclear counter stain is DAPI (blue).

 Negative control 1: ab300645 at 1/100 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at 1/1000 dilution.

Negative control 2: ab183830 at 1/1000 dilution followed by ab98736 Goat F(ab')2 Anti-Mouse IgG - Fc (DyLight^® 488) pre-adsorbed at 1/1000 dilution.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned mouse cerebellum tissue labeling MAP2 with ab300645 at 1/100 dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (green). Positive staining on mouse cerebellum is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution.

No antigen retrieval was carried out.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned rat cerebellum tissue labeling MAP2 with ab300645 at 1/100 dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (green). Positive staining on rat cerebellum is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution.

No antigen retrieval was carried out.