Anti-MAP2 抗体 [EPR19691] - Goat IgG (Chimeric) - BSA and Azide free

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Goat IgG (Chimeric) - BSA and Azide free (AB302488)

This data was developed using ab302487, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling MAP2 with ab302487 at 1/1000 (1.004 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (ab205723) at 1 : 2000 dilution. Positive staining on ganglion in the human colon. The section was incubated with ab302487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (ab205723) at 1 : 2000 dilution.Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Goat IgG (Chimeric) - BSA and Azide free (AB302488)

This data was developed using ab302487 , the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling MAP2 with ab302487 at 1/1000 (1.004 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (ab205723) at 1 : 2000 dilution. Positive staining on human cerebrum. The section was incubated with ab302487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (ab205723) at 1 : 2000 dilution.Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Goat IgG (Chimeric) - BSA and Azide free (AB302488)

This data was developed using ab302487, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling MAP2 with ab302487 at 1/1000 (1.004 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (ab205723) at 1 : 2000 dilution. Positive staining on ganglion in the rat colon. The section was incubated with ab302487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (ab205723) at 1 : 2000 dilution.Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] - Goat IgG (Chimeric) - BSA and Azide free (AB302488)

This data was developed using ab302487, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cells labeling MAP2 with ab302487 at 1/100 dilution (10.04 μg/ml), followed by ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 μg/mL) (Green). Confocal image showing cytoplasmic staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. ab183830 Anti-MAP2 rabbit monoclonal antibody was used to counterstain tubulin at 1/1000 dilution (1 μg/mL) (Red). The Nuclear counterstain was DAPI (Blue). The negative controls are as follows : -ve control 1 : ab302487 was used as primary antibody at 1/100 dilution, followed by ab150080 at 1/1000 dilution (2 μg/mL).  -ve control 2 : ab183830 at 1/200 dilution (10 μg/mL) was used as a primary antibody, followed by ab150129 at 1/1000 (2 μg/mL).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Goat IgG (Chimeric) - BSA and Azide free (AB302488)

This data was developed using ab302487, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling MAP2 with ab302487 at 1/1000 (1.004 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (ab205723) at 1 : 2000 dilution. Positive staining on rat cerebrum. The section was incubated with ab302487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (ab205723) at 1 : 2000 dilution.Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] - Goat IgG (Chimeric) - BSA and Azide free (AB302488)

Composite multiplex immunofluorescence staining of ab302487, ab309493 and ab254351 staining MAP2, Synaptophysin and SV2A in Mouse Primary Neurons DIV14 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal donkey serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab289874 (shown in yellow), ab309493 (shown in green) and ab254351 (shown in magenta) at 5µg/ml. Cells were then incubated with ab150136 Donkey Anti-Goat IgG H&L (Alexa Fluor® 594) preadsorbed, ab150111 Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed and ab150061 Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Goat IgG (Chimeric) - BSA and Azide free (AB302488)

This data was developed using ab302487, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling MAP2 with ab302487 at 1/1000 (1.004 ug/ml) followed by a ready to use . Secondary antibody : Donkey Anti-Goat IgG H&L (HRP) (ab205723).Positive staining on mouse cerebrum. The section was incubated with ab302487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use .Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Goat IgG (Chimeric) - BSA and Azide free (AB302488)

This data was developed using ab302487, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling MAP2 with ab302487 at 1/1000 (1.004 ug/ml) followed by a Donkey Anti-Goat IgG H&L (HRP) (ab205723) at 1 : 2000 dilution. Positive staining on ganglion in the mouse colon. The section was incubated with ab302487 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is Donkey Anti-Goat IgG H&L (HRP) (ab205723) at 1 : 2000 dilution.Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] - Goat IgG (Chimeric) - BSA and Azide free (AB302488)

This data was developed using ab302487, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cells labeling MAP2 with ab302487 at 1/100 dilution (10.04 μg/ml), followed by ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 μg/mL) (Green). Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. ab183830 Anti-MAP2 rabbit monoclonal antibody was used to counterstain tubulin at 1/1000 dilution (1 μg/mL) (Red). The Nuclear counterstain was DAPI (Blue). The negative controls are as follows : -ve control 1 : ab302487 was used as primary antibody at 1/100 dilution, followed by an150080 at 1/1000 dilution (2 μg/mL).  -ve control 2 : ab183830 at 1/200 dilution (10 μg/mL) was used as a primary antibody, followed by ab150129 at 1/1000 (2 μg/mL).