Anti-MAP2 抗体 [AP-20] (ab11268)
![Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [AP-20] (ab11268)](https://www.abcam.co.jp/ps/products/11/ab11268/Images/ab11268-18867-anti-map2-antibody-ap-20-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [AP-20] (ab11268)")
Key features and details
- Mouse monoclonal [AP-20] to MAP2
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse
- Isotype: IgG1
製品の概要
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製品名
Anti-MAP2 antibody [AP-20]
MAP2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [AP-20] to MAP2 -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse -
免疫原
Full length native protein (purified) corresponding to Cow MAP2.
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ポジティブ･コントロール
- ICC: Mouse Cell (Cortical neuron culture)
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特記事項
This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016- GR143561. Lot numbers higher than GR143561 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Proprietary Purification -
特記事項（精製）
Purified from Tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
AP-20 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11268の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration.
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| IHC-P | (1) |
1/5000.
Use at an assay dependent concentration. |
| 特記事項 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
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IHC-P
1/5000. Use at an assay dependent concentration. |
ターゲット情報
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機能
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. -
配列類似性
Contains 3 Tau/MAP repeats. -
翻訳後修飾
Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. -
細胞内局在
Cytoplasm, cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 17756 Mouse
- SwissProt: P20357 Mouse
- Unigene: 256966 Mouse
- Unigene: 436793 Mouse
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別名
- DKFZp686I2148 antibody
- MAP 2 antibody
- MAP dendrite specific antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [AP-20] (ab11268)This image is courtesy of Randal Moldrich, Queensland Brain Institute, AustraliaMouse monoclonal [AP-20] to MAP2 - Neuronal Marker (ab11268) used at a dilution of 1/500 in Immunocytochemistry/Immunofluorescence on Mouse Cell (Cortical neuron culture). Fixative: Paraformaldehyde. Blocking step: 2% Serum incubated for 1 hour at RT. Secondary Antibody: Goat anti-mouse AlexaFluor 594 Conjugation: Alexa Fluor® 594; 1/1000
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [AP-20] (ab11268)](https://www.abcam.co.jp/ps/products/11/ab11268/Images/ab11268-18866-anti-map2-antibody-ap-20-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [AP-20] (ab11268)This image is courtesy of Randal Moldrich, Queensland Brain Institute, Australiaab11268 staining MAP2 in E16 mouse brain neocortex (bottom is dorsal) by immunohistochemistry. Free-floating sections were incubated with ab11268 (1/5000) for 2 hours at 21ºC. Bound antibody was detected using a biotinylated donkey anti-mouse IgG antibody. This image is courtesy of an Abreview submitted by Randal Moldrich, 6 March 2008.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (36)
ab11268 は 36 報の論文で使用されています。
- Yang RY et al. Knockdown of polypyrimidine tract binding protein facilitates motor function recovery after spinal cord injury. Neural Regen Res 18:396-403 (2023). PubMed: 35900436
- Shikanai M et al. Rab21 regulates caveolin-1-mediated endocytic trafficking to promote immature neurite pruning. EMBO Rep 24:e54701 (2023). PubMed: 36683567
- Ali H et al. Attenuation hotspots in neurotropic human astroviruses. PLoS Biol 21:e3001815 (2023). PubMed: 37459343
- Solana-Balaguer J et al. Neuron-derived extracellular vesicles contain synaptic proteins, promote spine formation, activate TrkB-mediated signalling and preserve neuronal complexity. J Extracell Vesicles 12:e12355 (2023). PubMed: 37743539
- Solana-Balaguer J et al. RTP801 mediates transneuronal toxicity in culture via extracellular vesicles. J Extracell Vesicles 12:e12378 (2023). PubMed: 37932242