Anti-MAP2 抗体 (ab5392)
Key features and details
- Chicken polyclonal to MAP2
- Suitable for: ICC, IHC-P, WB
- Reacts with: Mouse, Rat
- Isotype: IgY
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-MAP2 antibody
MAP2 一次抗体 製品一覧 -
製品の詳細
Chicken polyclonal to MAP2 -
由来種
Chicken -
アプリケーション
適用あり: ICC, IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat -
免疫原
Recombinant fragment corresponding to Human MAP2. Mix of recombinant human constructs of projection domain sequences, amino acids 235-1588.
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ポジティブ･コントロール
- IHC-P: Rat cerebellum tissue. ICC: Rat E20 cultured cortical neuron-glial cells, rat cortical neurons, rat cortical neurons and glia in mixed tissue culture and mouse neurons. WB: Adult rat brain, Embryonic E20 rat brain and Adult mouse brain lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
Preservative: 0.032% Sodium azide
Constituent: PBS -
Concentration information loading... -
特記事項（精製）
Lipid extraction from egg yolk, followed by ammonium sulphate precipitation. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgY -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5392の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC | (4) |
1/5000 - 1/10000.
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| IHC-P | (13) |
1/10000.
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| WB | (6) |
1/20000 - 1/100000.
This 280kDa band corresponds to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands. |
| 特記事項 |
|---|
|
ICC
1/5000 - 1/10000. |
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IHC-P
1/10000. |
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WB
1/20000 - 1/100000. This 280kDa band corresponds to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands. |
ターゲット情報
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機能
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. -
配列類似性
Contains 3 Tau/MAP repeats. -
翻訳後修飾
Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. -
細胞内局在
Cytoplasm, cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 17756 Mouse
- Entrez Gene: 25595 Rat
- SwissProt: P20357 Mouse
- SwissProt: P15146 Rat
- Unigene: 256966 Mouse
- Unigene: 436793 Mouse
- Unigene: 10484 Rat
- Unigene: 136301 Rat
see all -
別名
- DKFZp686I2148 antibody
- MAP 2 antibody
- MAP dendrite specific antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody (ab5392)
Chromogenic Immunostaining of a formalin fixed paraffin embedded rat cerebellum section with chicken pAb to MAP2A/B dilution 1/10000, detected in DAB (brown) following the ABC method. Hematoxylin (blue) was used as the counterstain. ab5392 detects neuronal cells, specifically their perikarya and dendrites. Note the prominent staining of Purkinje cells and their dendrites in the molecular layer and staining of the much smaller granule neurons in the granular layer.
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Immunocytochemistry - Anti-MAP2 antibody (ab5392)Rat E20 cultured cortical neuron-glial cells stained for MAP2 (red) using ab5392 at 1/10000 dilution for ICC/IF. Tau is detected with a mouse monoclonal anti-Tau antibody (green). The nuclear counter stain is DAPI (blue). Overlap of MAP2 and Tau staining results in an orange-yellow color.
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Western blot - Anti-MAP2 antibody (ab5392)All lanes : Anti-MAP2 antibody (ab5392) at 1/50000 dilution
Lane 1 : Adult rat brain lysate
Lane 2 : Embryonic E20 rat brain lysate
Lane 3 : Adult mouse brain lysate -
Immunocytochemistry - Anti-MAP2 antibody (ab5392)ab5392 at a dilution of 1/10000, staining MAP 2 (green) in tissue cultured rat cortical neurons by immunofluorescence. Nuclei are stained blue with Dapi. -
Immunocytochemistry - Anti-MAP2 antibody (ab5392)Rat cortical neurons and glia in mixed tissue culture stained with ab5392 (Chicken antibody to MAP2) (green)(1:30 000), a mouse monoclonal antibody to GFAP (red) and nuclei of all cells stained with Hoechst dye (blue). -
Immunocytochemistry - Anti-MAP2 antibody (ab5392)This image is courtesy of an anonymous Abreviewab5392 staining MAP2 in mouse neurons by immunocytochemistry/ immunofluorescence. Cells were PFA fixed and permeabilized in 0.4% Triton X-100 prior to blocking in 5% serum for 1 hour at 25°C. The primary antibody was diluted 1/10000 and incubated with the sample for 20 hours at 21°C. Alexa fluor® 488 goat polyclonal to chicken IgY, diluted 1/400, was used as the secondary antibody. -
Western blot - Anti-MAP2 antibody (ab5392)This image is courtesy of an anonymous AbreviewAnti-MAP2 antibody (ab5392) at 1/10000 dilution + Mouse brain lysate - post nuclear supernatant at 10 µg
Secondary
HRP-conjugated rabbit anti-chicken IgY at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 280,70 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (788)
ab5392 は 788 報の論文で使用されています。
- Yao Y et al. Schizophrenia-Like Behaviors Arising from Dysregulated Proline Metabolism Are Associated with Altered Neuronal Morphology and Function in Mice with Hippocampal PRODH Deficiency. Aging Dis 15:1952-1968 (2024). PubMed: 37815900
- Gale JR et al. Copper induces neuron-sparing, ferredoxin 1-independent astrocyte toxicity mediated by oxidative stress. J Neurochem 167:277-295 (2023). PubMed: 37702109
- Villa PA et al. Altered GnRH neuron and ovarian innervation characterize reproductive dysfunction linked to the Fragile X messenger ribonucleoprotein (Fmr1) gene mutation. Front Endocrinol (Lausanne) 14:1129534 (2023). PubMed: 36909303
- Wolzak K et al. Protein disulfide isomerases as CSF biomarkers for the neuronal response to tau pathology. Alzheimers Dement 19:3563-3574 (2023). PubMed: 36825551
- Feringa FM et al. An Atypical, Staged Cell Death Pathway Induced by Depletion of SNARE-Proteins MUNC18-1 or Syntaxin-1. J Neurosci 43:347-358 (2023). PubMed: 36517239