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AB254471

Anti-CD206抗体[EPR22489-7] - BSA and Azide free

Anti-CD206 antibody [EPR22489-7] - BSA and Azide free

5

(2 Reviews)

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(4 Publications)

Anti-CD206 antibody [EPR22489-7] - BSA and Azide free (ab254471) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting CD206 in Western Blot, IHC-P. Suitable for Human.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

別名を表示する

CD206, CLEC13D, CLEC13DL, MRC1L1, MRC1, Macrophage mannose receptor 1, MMR, C-type lectin domain family 13 member D, C-type lectin domain family 13 member D-like, Human mannose receptor, Macrophage mannose receptor 1-like protein 1, hMR

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD206 antibody [EPR22489-7] - BSA and Azide free (AB254471)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD206 antibody [EPR22489-7] - BSA and Azide free (AB254471)

Immunohistochemical analysis of paraffin-embedded human Karposi's sarcoma tissue labeling CD206 with ab252921 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in tumor cells of human Kaposi's Sarcoma (PMID : 9060831) is observed. Counterstained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252921).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD206 antibody [EPR22489-7] - BSA and Azide free (AB254471)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD206 antibody [EPR22489-7] - BSA and Azide free (AB254471)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD206 with ab252921 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in macrophages (Kupffer cells) of human liver (PMID : 26938527) is observed. Counterstained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252921).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD206 antibody [EPR22489-7] - BSA and Azide free (AB254471)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD206 antibody [EPR22489-7] - BSA and Azide free (AB254471)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CD206 with ab252921 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in macrophages of human colon (PMID : 9060831) is observed. Counterstained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252921).

関連する標識済み抗体及び組成の異なる製品 (4)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR22489-7

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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代替製品

Primary Antibodies

AB125028

Anti-CD206 antibody [EPR6828(B)]

RabMAb|Recombinant|20ul selling size|Trial Size|Trial Size

Western blot - Anti-CD206 antibody [EPR6828(B)] (AB125028)

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Primary Antibodies

AB209327

Anti-CD206 antibody [EPR6828(B)] - Low endotoxin, Azide free

RabMAb|Recombinant

Western blot - Anti-CD206 antibody [EPR6828(B)] - Low endotoxin, Azide free (AB209327)

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組合せ製品

Assay Kits

AB277420

Human CD206 ELISA kit

ELISA - Human CD206 ELISA kit (AB277420)

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Sample Prep & Detection Kits

AB188287

Cy3® Conjugation Kit (Fast) - Lightning-Link®

Fluorescence Resonance Energy Transfer - Cy3® Conjugation Kit (Fast) - Lightning-Link® (AB188287)

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Sample Prep & Detection Kits

AB269892

Latex Conjugation Kit - 400nm Blue

Functional Studies - Latex Conjugation Kit - 400nm Blue (AB269892)

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Primary Antibodies

AB213363

Anti-CD68 antibody [EPR20545]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-CD68 antibody [EPR20545] (AB213363)

  • 4
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Primary Antibodies

AB243887

Anti-CD86 antibody [BLR030F] - BSA free

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [BLR030F] - BSA free (AB243887)

  • 0
  • 0
Primary Antibodies

AB182422

Anti-CD163 antibody [EPR19518]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Flow Cytometry - Anti-CD163 antibody [EPR19518] (AB182422)

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Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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製品の詳細

What is this antibody validated in?
Anti-CD206 antibody [EPR22489-7] - BSA and Azide free (ab254471) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of CD206?
Anti-CD206 [EPR22489-7] - BSA and Azide free (ab254471) specifically detects a band for CD206 (UniProt: P22897) at a molecular weight of 166kDa.

Other related products
We have a range of other formats of antibody clone [EPR22489-7] also available for your convenience: ab252921, Carrier free - ab254471, Alexa Fluor® 488 - ab313398, Alexa Fluor® 647 - ab313671, Alexa Fluor® 555 - ab313731

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The CD206 or MRC1 is a type of C-type lectin receptor with a molecular weight of 175 kDa. It is expressed on the surface of macrophages and dendritic cells. This receptor binds to complex carbohydrates like mannose and fucose found on pathogen surfaces playing an important role in their recognition. CD206 acts as endocytic receptors aiding in the internalization and degradation of pathogens.
Biological function summary

The CD206 functions in pathogen recognition and clearance by binding to specific carbohydrates on the pathogen's surface. It is not part of a larger molecular complex but works independently to facilitate phagocytosis. The receptor is essential in immune responses allowing macrophages and dendritic cells to efficiently capture and process antigens for presentation to T cells. This enables the initiation of adaptive immune responses.

Pathways

Researchers know that the CD206 plays an important role in immune signaling pathways particularly in antigen processing and presentation. It interacts with proteins involved in these pathways including those necessary for the internalization and degradation of bound antigens. Moreover it works alongside scavenger receptors contributing to the regulation of immune and inflammatory responses.

The CD206 is often linked to pulmonary tuberculosis and certain types of cancer. When macrophages express this receptor it can modulate immune responses against Mycobacterium tuberculosis by aiding in its recognition. In cancer altered expression of CD206 can indicate tumor-associated macrophages which promote tumor growth by suppressing immune responses. These connections highlight the receptor's potential as a diagnostic marker and therapeutic target.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains.. (Microbial infection) Acts as a phagocytic receptor for bacteria, fungi and other pathogens.. (Microbial infection) Acts as a receptor for Dengue virus envelope protein E.. (Microbial infection) Interacts with Hepatitis B virus envelope protein.
See full target information MRC1
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Nature immunology 26:1182-1197 PubMed40588561

2025

Chemosensor receptors are lipid-detecting regulators of macrophage function in cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Giulia Marelli,Nicolò Morina,Simone Puccio,Marta Iovino,Marta Pandini,Federica Portale,Mattia Carvetta,Divya Mishra,Elisabetta Diana,Greta Meregalli,Elvezia Paraboschi,Javier Cibella,Clelia Peano,Gianluca Basso,Gabriele De Simone,Chiara Camisaschi,Elena Magrini,Giulio Sartori,Elham Karimi,Piergiuseppe Colombo,Massimo Lazzeri,Paolo Casale,Lavinia Morosi,Giuseppe Martano,Rosanna Asselta,Eduardo Bonavita,Hiro Matsunami,Francesco Bertoni,Logan Walsh,Enrico Lugli,Diletta Di Mitri

Frontiers in immunology 16:1508559 PubMed40078987

2025

Single-cell RNA-sequencing reveals cellular heterogeneity and immune microenvironment characteristics between ocular adnexal mucosa-associated lymphoid lymphoma and IgG4-related ophthalmic disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Yang,Yujiao Wang,Xuelian Jin,Weimin He

Cell reports methods 3:100595 PubMed37741277

2023

Dual-modality imaging of immunofluorescence and imaging mass cytometry for whole-slide imaging and accurate segmentation.

Applications

Unspecified application

Species

Unspecified reactive species

Eun Na Kim,Phyllis Zixuan Chen,Dario Bressan,Monika Tripathi,Ahmad Miremadi,Massimiliano di Pietro,Lisa M Coussens,Gregory J Hannon,Rebecca C Fitzgerald,Lizhe Zhuang,Young Hwan Chang

Frontiers in bioengineering and biotechnology 10:816513 PubMed35402413

2022

Photooxidation and Pentagalloyl Glucose Cross-Linking Improves the Performance of Decellularized Small-Diameter Vascular Xenograft .

Applications

Unspecified application

Species

Unspecified reactive species

Yuhong Liu,Chunyang Chen,Xinlong Xie,Haoyong Yuan,Zhenjie Tang,Tao Qian,Yalin Liu,Mingzhe Song,Sixi Liu,Ting Lu,Zhongshi Wu
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
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