Anti-Mannose Receptor 抗体 (ab64693)
Key features and details
- Rabbit polyclonal to Mannose Receptor
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Mannose Receptor antibody
Mannose Receptor 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Mannose Receptor -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chimpanzee, Rhesus monkey -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 1400 to the C-terminus of Human Mannose Receptor.
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ポジティブ・コントロール
- WB: Rat liver and lung, human lung, mouse lung tissue lysate. IHC-P: Human, rat and mouse lung tissue. ICC/IF: MOLT-4 whole cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64693の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (24) |
Use a concentration of 0.05 - 0.1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
We do not recommend methanol fixation with this antibody |
WB | (3) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 166 kDa.
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ICC/IF | (1) |
Use a concentration of 1 µg/ml.
Permeabilise with Tween (0.1%) |
特記事項 |
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IHC-P
Use a concentration of 0.05 - 0.1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. We do not recommend methanol fixation with this antibody |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 166 kDa. |
ICC/IF
Use a concentration of 1 µg/ml. Permeabilise with Tween (0.1%) |
ターゲット情報
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機能
Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens. -
配列類似性
Contains 8 C-type lectin domains.
Contains 1 fibronectin type-II domain.
Contains 1 ricin B-type lectin domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4360 Human
- Entrez Gene: 17533 Mouse
- Entrez Gene: 291327 Rat
- Omim: 153618 Human
- SwissProt: P22897 Human
- SwissProt: Q61830 Mouse
- Unigene: 75182 Human
- Unigene: 2019 Mouse
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別名
- bA541I19.1 antibody
- C type lectin domain family 13 member D antibody
- C-type lectin domain family 13 member D antibody
see all
画像
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All lanes : Anti-Mannose Receptor antibody (ab64693) at 1 µg/ml
Lane 1 : Rat liver tissue lysate
Lane 2 : Human lung tissue lysate
Lane 3 : Rat lung tissue lysate
Lane 4 :Mouse lung normal tissue lysate - total protein (ab29297)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 166 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes -
ab64693 staining Mannose Receptor in MOLT4 cells. The cells were fixed with 4% Formaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab64693 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)
IHC image of ab64693 staining in human lung formalin fixed paraffin embedded tissue section*, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64693, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)
Immunohistochemical analysis of formalin fixed paraffin embedded human lung labelling mannose receptor with ab64693 at 0.1 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32min with ULTRA cell conditioning solution (CC1 pH8.5). ab64693 anti mannose receptor antibody was incubated at 37°C for 16min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)This image is courtesy of an anonymous Abreview
Paraffin embedded, formalin-fixed mouse lung tissue labelling the mannose receptor with ab64693 at 1/500 in immunohistochemical analysis. An Alexa Fluor® 647 Goat anti-rabbit IgG was used as the secondary antibody, this was artificially colored green by software to enhance stain visibility. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. 15% serum was used as blocking agent for 1 hour at room temperature.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)
IHC image of Mannose Receptor staining in a section of formalin-fixed paraffin-embedded normal rat lung performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab64693, 0.01ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)
IHC image of Mannose Receptor staining in a section of formalin-fixed paraffin-embedded normal mouse lung performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab64693, 0.01ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (750)
ab64693 は 750 報の論文で使用されています。
- Huang S et al. Genetic and Immunohistochemistry Tools to Visualize Rat Macrophages In Situ. Methods Mol Biol 2713:99-115 (2024). PubMed: 37639117
- Fan Z et al. Simulation of Cortical and Cancellous Bone to Accelerate Tissue Regeneration. Adv Funct Mater 33:N/A (2023). PubMed: 37601745
- Yang H et al. Dapagliflozin promotes angiogenesis in hindlimb ischemia mice by inducing M2 macrophage polarization. Front Pharmacol 14:1255904 (2023). PubMed: 37808194
- Wilkinson AL et al. The senescent secretome drives PLVAP expression in cultured human hepatic endothelial cells to promote monocyte transmigration. iScience 26:107966 (2023). PubMed: 37810232
- Fu J et al. Polydopamine (PDA) coatings with endothelial vascular growth factor (VEGF) immobilization inhibiting neointimal formation post zinc (zn) wire implantation in rat aortas. Biomater Res 27:84 (2023). PubMed: 37667399