Anti-MALT1/MLT 抗体 [EPR604]

• WB

Lab

Western blot - Anti-MALT1/MLT antibody [EPR604] (AB109724)

Western blot : Anti-MALT1/MLT antibody [EPR604] ab109724 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 92 kDa in Wild-type HCT 116 cell lysates with no signal observed at this size in MALT1 knockout HCT 116 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-MALT1/MLT antibody [EPR604] (ab109724) at 1/1000 dilution

Lane 1:

Western blot - Human wild-type HCT116 cell line (ab288559) at 20 µg

Lane 2:

Western blot - Human MALT1 knockout HCT116 cell line (<a href='/products/cell-lines/human-malt1-knockout-hct116-cell-line-ab286597'>ab286597</a>) at 20 µg

Lane 3:

HeLa at 20 µg

Lane 4:

Ramos at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 92 kDa

Observed band size: 92 kDa

false

• WB

Unknown

Western blot - Anti-MALT1/MLT antibody [EPR604] (AB109724)

All lanes:

Western blot - Anti-MALT1/MLT antibody [EPR604] (ab109724) at 1/1000 dilution

Lane 1:

Jurkat lysate at 10 µg

Lane 2:

K562 lysate at 10 µg

Lane 3:

Bone marrow lysate at 10 µg

Lane 4:

PBMC lysates at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 92 kDa

false