Anti-Malignant fibrous histiocytoma 抗体 (ab185289)
Key features and details
- Rabbit polyclonal to Malignant fibrous histiocytoma
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Malignant fibrous histiocytoma antibody -
製品の詳細
Rabbit polyclonal to Malignant fibrous histiocytoma -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Recombinant fragment corresponding to Human Malignant fibrous histiocytoma aa 950-1050.
Database link: Q9Y4C4 -
ポジティブ･コントロール
- IHC-P:Human rectum tissue; WB: RT-4 and U-251 MG whole cell lysate; ICC: U-2 OS whole cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185289の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 117 kDa.
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| IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 117 kDa. |
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IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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組織特異性
Ubiquitously expressed. Overexpressed in malignant fibrous histiocytomas. -
関連疾患
A chromosomal aberration involving MFHAS1 may be a cause of B-cell lymphoma. Translocation t(8;14)(p23.1;q21) with a cryptic exon named '14q21 element'. The resulting fusion protein named 'chimeric MASL1' is tumorigenic in nude mice. -
配列類似性
Contains 13 LRR (leucine-rich) repeats.
Contains 1 Roc domain. - Information by UniProt
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参照データベース
- Entrez Gene: 9258 Human
- Entrez Gene: 52065 Mouse
- Entrez Gene: 306508 Rat
- SwissProt: Q9Y4C4 Human
- SwissProt: Q3V1N1 Mouse
- Unigene: 379414 Human
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別名
- Malignant fibrous histiocytoma-amplified sequence 1 antibody
- Malignant fibrous histiocytoma-amplified sequence with leucine-rich tandem repeats 1 antibody
- MFHA1_HUMAN antibody
- Mfhas1 antibody
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Malignant fibrous histiocytoma antibody (ab185289)
Immunohistochemical analysis of paraffin-embedded human rectum tissue labeling malignant fibrous histiocytoma in the nucleus and cytoplasm of glandular cells with ab185289 at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-Malignant fibrous histiocytoma antibody (ab185289)All lanes : Anti-Malignant fibrous histiocytoma antibody (ab185289) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) whole cell lysate
Predicted band size: 117 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Malignant fibrous histiocytoma antibody (ab185289)Immunocytochemistry analysis of U-2 OS (human bone osteosarcoma epithelial cell line) whole cells labeling malignant fibrous histiocytoma in the nucleoplasm and cytosol with ab185289 at 2 µg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab185289 は 1 報の論文で使用されています。
- Lian H et al. Malignant fibrous histiocytoma amplified sequence 1 alleviates inflammation and renal fibrosis in diabetic nephropathy by inhibiting TLR4. Biosci Rep 39:N/A (2019). PubMed: 31696221