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リコンビナントRabMAb

Anti-MAGEA1 抗体 [SP194] - BSA and Azide free (ab245755)

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Flow Cytometry (Intracellular) - Anti-MAGEA1 antibody [SP194] - BSA and Azide free (ab245755)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA1 antibody [SP194] - BSA and Azide free (ab245755)
  • Flow Cytometry (Intracellular) - Anti-MAGEA1 antibody [SP194] - BSA and Azide free (ab245755)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA1 antibody [SP194] - BSA and Azide free (ab245755)
  • Anti-MAGEA1 antibody [SP194] - BSA and Azide free (ab245755)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP194] to MAGEA1 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-MAGEA1 antibody [SP194] - BSA and Azide free
    MAGEA1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [SP194] to MAGEA1 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), IHC-P, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human HeLa, SK-OV-3 and A431 cell lysates. Human A431 cells. Human testis and skin squamous cell carcinoma tissues.

  • 特記事項

    ab245755 is the carrier-free version of ab183309.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab245755の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 34 kDa.
特記事項
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 34 kDa.

ターゲット情報

  • 機能

    May be involved in transcriptional regulation through interaction with SNW1 and recruiting histone deactelyase HDAC1. May inhibit notch intracellular domain (NICD) transactivation. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.

  • 組織特異性

    Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes. Never expressed in kidney tumors, leukemias and lymphomas.

  • 配列類似性

    Contains 1 MAGE domain.

  • 細胞内局在

    Cytoplasm. Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Antigen MZ2 E antibody
      • Antigen MZ2-E antibody
      • Cancer/testis antigen 1.1 antibody
      • cancer/testis antigen family 1, member 1 antibody
      • CT1.1 antibody
      • MAGA1_HUMAN antibody
      • MAGE 1 antigen antibody
      • MAGE-1 antigen antibody
      • MAGE1 antibody
      • MAGE1A antibody
      • MAGEA1 antibody
      • Melanoma antigen 1 antibody
      • Melanoma antigen family A 1 (directs expression of antigen MZ2 E) antibody
      • Melanoma antigen family A 1 antibody
      • Melanoma antigen MAGE 1 antibody
      • Melanoma associated antigen 1 antibody
      • Melanoma associated antigen MZ2 E antibody
      • Melanoma-associated antigen 1 antibody
      • MGC9326 antibody
      see all

    画像

    • Flow Cytometry (Intracellular) - Anti-MAGEA1 antibody [SP194] - BSA and Azide free (ab245755)
      Flow Cytometry (Intracellular) - Anti-MAGEA1 antibody [SP194] - BSA and Azide free (ab245755)

      Intracellular Flow Cytometry analysis of SK-OV-3 (Human ovarian cancer epithelial cell) cells labeling MAGEA1 with purified ab183309 at 1/20 dilution (8µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183309). 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA1 antibody [SP194] - BSA and Azide free (ab245755)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA1 antibody [SP194] - BSA and Azide free (ab245755)

      Immunohistochemical analysis of Human skin squamous cell carcinoma tissue labeling MAGEA1 using ab183309 at a 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183309).

    • Flow Cytometry (Intracellular) - Anti-MAGEA1 antibody [SP194] - BSA and Azide free (ab245755)
      Flow Cytometry (Intracellular) - Anti-MAGEA1 antibody [SP194] - BSA and Azide free (ab245755)

      Intracellular flow cytometric analysis of Human A431 cells labeling MAGEA1 using ab183309 (1/400) (green) compared to a negative control rabbit IgG (blue). 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183309). 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA1 antibody [SP194] - BSA and Azide free (ab245755)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA1 antibody [SP194] - BSA and Azide free (ab245755)

      Immunohistochemical analysis of Human testis tissue labeling MAGEA1 using ab183309 at a 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183309).

    • Anti-MAGEA1 antibody [SP194] - BSA and Azide free (ab245755)
      Anti-MAGEA1 antibody [SP194] - BSA and Azide free (ab245755)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab245755 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab245755 は論文での使用が確認できていません。

    レビューと Q&A

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com