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AB140678

Anti-MAGE3 抗体 [OTI1H1]

Anti-MAGE3 antibody [OTI1H1]

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(2 Publications)

Mouse Monoclonal MAGE3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MAGEA3.

別名を表示する

MAGE3, MAGEA3, Melanoma-associated antigen 3, Antigen MZ2-D, Cancer/testis antigen 1.3, MAGE-3 antigen, CT1.3

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

OTI1H1

アイソタイプ

IgG1

キャリアフリー

No

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

Recombinant Full Length Protein corresponding to Human MAGEA3.

P43357

特異性

This antibody cross reacts with MAGEA2, MAGEA6, MAGEA8 and MAGEA12 and does not cross react with MAGEA1 and MAGEA4.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p></p>" } } }
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製品の詳細

The clone number has been updated from 1H1 to OTI1H1, both clone numbers name the same clone.

Dilute in PBS (pH7.3) before use.

This product was changed from ascites to tissue culture supernatant on 10th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein G
精製に関する特記事項
ab140678 is purified from TCS by affinity chromatography.
バッファー組成
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 48% PBS, 1% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The 'MAGE-A3' protein sometimes referred to simply as 'MAGE3' is part of the MAGE (Melanoma Antigen Gene) family which comprises a group of proteins known for their involvement in various cellular processes. MAGE-A3 specifically is encoded by a gene located on the X chromosome. The molecular mass of MAGE-A3 is approximately 46 kDa. This protein is typically expressed in a variety of tumor cells but is absent in most normal tissues with the exception of placental and testicular tissues. Its expression profile makes MAGE-A3 a significant focus in cancer research due to its restricted presence in normal cells and prevalent presence in transformed cells.
Biological function summary

MAGE-A3 contributes to processes related to cell cycle regulation and apoptosis. The protein is part of a complex that influences cellular stress responses and survival pathways. It exerts its biological function through interactions with other proteins potentially acting as a regulator of transcription factors involved in cell proliferation and survival. The activity and utility of MAGE-A3 in this context make it a target of interest in therapeutic research specifically for cancer vaccines and immune response strategies.

Pathways

MAGE-A3 plays a role in the regulation of the apoptotic and cell survival pathways. It is notably involved in pathways such as the p53 pathway essential for modulating cellular responses to stress and DNA damage. MAGE-A3 interacts with proteins like p53 and other regulators involved in the cell cycle and apoptosis thereby affecting cellular decisions to repair damage or undergo programmed cell death. Its role in these pathways highlights its importance in maintaining the balance between cell survival and death highlighting its potential target for therapeutic intervention.

MAGE-A3 is closely associated with cancers such as melanoma and non-small cell lung cancer. Its expression in these malignancies makes it a prime candidate for cancer-specific targeted therapies including cancer vaccines. MAGE-A3 is connected with proteins that regulate immune evasion and tumor growth making its modulation an attractive strategy in immunotherapy. Understanding the interaction of MAGE-A3 with these proteins provides valuable insights necessary for developing precise diagnostic and treatment methods for these cancers.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Activator of ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases that acts as a repressor of autophagy (PubMed : 20864041, PubMed : 31267705). May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3 : substrate complex (PubMed : 17942928, PubMed : 20864041). May play a role in embryonal development and tumor transformation or aspects of tumor progression (PubMed : 17942928, PubMed : 20864041). In vitro promotes cell viability in melanoma cell lines (PubMed : 17942928). Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes (PubMed : 8113684).
See full target information MAGEA3
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer letters 435:1-9 PubMed30056111

2018

miR-1273g silences MAGEA3/6 to inhibit human colorectal cancer cell growth via activation of AMPK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Wu,Fang Liu,Lemei Dong,Han Yang,Xixi He,Lili Li,Lihao Zhao,Sisi Jin,Gang Li

Oncology letters 13:1609-1618 PubMed28454298

2017

Expression and prognostic relevance of MAGE-A3 and MAGE-C2 in non-small cell lung cancer.

Applications

IHC-P

Species

Human

Xinfeng Chen,Liping Wang,Jinyan Liu,Lan Huang,Li Yang,Qun Gao,Xiaojuan Shi,Jieyao Li,Feng Li,Zhen Zhang,Song Zhao,Bin Zhang,Pierre Van der Bruggen,Yi Zhang
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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