Anti-MAFK 抗体 (ab50322)
Key features and details
- Rabbit polyclonal to MAFK
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MAFK antibody
MAFK 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MAFK -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chicken, Zebrafish
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免疫原
Synthetic peptide:
TTNPKPNKALKVKKEA
conjugated to KLH by a C-terminal Cysteine residue linker, corresponding to amino acids 2-17 of Human MAFK -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
精製度
Ion Exchange Chromatography -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50322の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/4000 - 1/8000. Predicted molecular weight: 17 kDa.
Dilute the antibody in PBS containing 5.0% non-fat dry milk and 0.05 % Tween 20. Staining of the MAFF band in immunoblotting is specifically inhibited by the immunizing peptide. |
| 特記事項 |
|---|
|
WB
1/4000 - 1/8000. Predicted molecular weight: 17 kDa. Dilute the antibody in PBS containing 5.0% non-fat dry milk and 0.05 % Tween 20. Staining of the MAFF band in immunoblotting is specifically inhibited by the immunizing peptide. |
ターゲット情報
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機能
Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. -
配列類似性
Belongs to the bZIP family. Maf subfamily.
Contains 1 bZIP domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 395518 Chicken
- Entrez Gene: 7975 Human
- Entrez Gene: 17135 Mouse
- Entrez Gene: 246760 Rat
- Entrez Gene: 415133 Zebrafish
- Omim: 600197 Human
- SwissProt: Q90596 Chicken
- SwissProt: O60675 Human
see all -
別名
- Erythroid transcription factor NF E2 p18 subunit antibody
- Erythroid transcription factor NF-E2 p18 subunit antibody
- FLJ32205 antibody
see all
画像
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Western blot - Anti-MAFK antibody (ab50322)Lanes 1 & 3 : Anti-MAFK antibody (ab50322) at 1/4000 dilution
Lane 2 : Anti-MAFK antibody (ab50322) at 1/8000 dilution
Lane 4 : no primary antibody
Lanes 1-2 & 4 : A431 whole cell lysate
Lane 3 : A431 whole cell lysate with MAFF immunizing peptide at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate
Predicted band size: 17 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab50322 は 8 報の論文で使用されています。
- Shen H et al. ZEB1-induced LINC01559 expedites cell proliferation, migration and EMT process in gastric cancer through recruiting IGF2BP2 to stabilize ZEB1 expression. Cell Death Dis 12:349 (2021). PubMed: 33824282
- Johnson GS et al. A functional pseudogene, NMRAL2P, is regulated by Nrf2 and serves as a coactivator of NQO1 in sulforaphane-treated colon cancer cells. Mol Nutr Food Res 61:N/A (2017). PubMed: 27860235
- Zheng S et al. TAM receptor-dependent regulation of SOCS3 and MAPKs contributes to proinflammatory cytokine downregulation following chronic NOD2 stimulation of human macrophages. J Immunol 194:1928-37 (2015). PubMed: 25567680
- Zhang Z et al. Oxidative stress regulates CFTR gene expression in human airway epithelial cells through a distal antioxidant response element. Am J Respir Cell Mol Biol 52:387-96 (2015). PubMed: 25259561
- Marinov GK et al. Evidence for site-specific occupancy of the mitochondrial genome by nuclear transcription factors. PLoS One 9:e84713 (2014). ICC/IF ; Human . PubMed: 24465428
- Hwang YJ et al. Oleanolic acid regulates NF-?B signaling by suppressing MafK expression in RAW 264.7 cells. BMB Rep 47:524-9 (2014). PubMed: 25059280
- Jolma A et al. DNA-binding specificities of human transcription factors. Cell 152:327-39 (2013). PubMed: 23332764
- Yang D et al. Identification of KAP-1-associated complexes negatively regulating the Ey and ß-major globin genes in the ß-globin locus. J Proteomics 80C:132-144 (2013). ChIP . PubMed: 23291531