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Anti-MAdCAM1 抗体 [AP-MAB0842] (ab80680)

Reviews (1) Submit a question References (1)

Key features and details

  • Rat monoclonal [AP-MAB0842] to MAdCAM1
  • Suitable for: IHC-Fr, IP, IHC-P, Flow Cyt, Inhibition Assay
  • Reacts with: Mouse
  • Isotype: IgG2

製品の概要

  • 製品名

    Anti-MAdCAM1 antibody [AP-MAB0842]
    MAdCAM1 一次抗体 製品一覧

  • 製品の詳細

    Rat monoclonal [AP-MAB0842] to MAdCAM1

  • 由来種

    Rat

  • アプリケーション

    適用あり: IHC-Fr, IP, IHC-P, Flow Cyt, Inhibition Assaymore details

  • 種交差性

    交差種: Mouse

  • 免疫原

    Tissue, cells or virus corresponding to Mouse MAdCAM1.
    Database link: Q61826

  • 特記事項


    Endotoxin Level: Extremely low level of LPS (< 0.002EU/µg IgG).


    ab80680 was produced by in vitro culture, from a hybridoma (mouse myeloma fused with spleen cells from an immunized rat).
    ab80680 is useful for reducing T cell mediated inflammation in gastrointestinal diseases.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab80680の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P (1)
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
Inhibition Assay
Use at an assay dependent concentration.

ab80680 is useful for blocking the interaction of MAdCAM1 with its counter receptor.
特記事項
IHC-Fr
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
Inhibition Assay
Use at an assay dependent concentration.

ab80680 is useful for blocking the interaction of MAdCAM1 with its counter receptor.

ターゲット情報

  • 機能

    Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.

  • 組織特異性

    Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.

  • 配列類似性

    Contains 2 Ig-like (immunoglobulin-like) domains.

  • 翻訳後修飾

    The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Addressin mucosal antibody
      • hMAdCAM 1 antibody
      • hMAdCAM-1 antibody
      • MACAM1 antibody
      • MADCA_HUMAN antibody
      • MAdCAM 1 antibody
      • MAdCAM-1 antibody
      • Madcam1 antibody
      • Mucosal addressin cell adhesion molecule 1 antibody
      • Mucosal vascular addressin cell adhesion molecule 1 antibody
      see all

    参考文献 (1)

    ab80680 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab80680 は 1 報の論文で使用されています。

    • Gao RY  et al. Bile acids modulate colonic MAdCAM-1 expression in a murine model of combined cholestasis and colitis. Mucosal Immunol 14:479-490 (2021). PubMed: 33004979

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MAdCAM1 antibody [AP-MAB0842]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    (agent) for 15 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
    Sample
    Mouse Tissue sections (Colon)
    Specification
    Colon
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Kaitlin Myrick

    Verified customer

    投稿 Jul 28 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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