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Anti-MAdCAM1 抗体 [314G8] (ab34247)

Submit a review Q&A (2)References (1)

Key features and details

  • Mouse monoclonal [314G8] to MAdCAM1
  • Suitable for: ELISA, IHC-Fr, IHC-P, Functional Studies, Flow Cyt, WB
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-MAdCAM1 antibody [314G8]
    MAdCAM1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [314G8] to MAdCAM1

  • 由来種

    Mouse

  • アプリケーション

    適用あり: ELISA, IHC-Fr, IHC-P, Functional Studies, Flow Cyt, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Fusion protein corresponding to Human MAdCAM1.
    Database link: Q13477

  • 特記事項


    Clone 314G8 reacts with the ligand binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognised by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM1 with alpha 4 beta 7 (see reference 1).

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab34247の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Functional Studies
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
Use at an assay dependent concentration.
特記事項
ELISA
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Functional Studies
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.

  • 組織特異性

    Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.

  • 配列類似性

    Contains 2 Ig-like (immunoglobulin-like) domains.

  • 翻訳後修飾

    The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Addressin mucosal antibody
      • hMAdCAM 1 antibody
      • hMAdCAM-1 antibody
      • MACAM1 antibody
      • MADCA_HUMAN antibody
      • MAdCAM 1 antibody
      • MAdCAM-1 antibody
      • Madcam1 antibody
      • Mucosal addressin cell adhesion molecule 1 antibody
      • Mucosal vascular addressin cell adhesion molecule 1 antibody
      see all

    参考文献 (1)

    ab34247 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab34247 は 1 報の論文で使用されています。

    • Allavena R  et al. CNS elevation of vascular and not mucosal addressin cell adhesion molecules in patients with multiple sclerosis. Am J Pathol 176:556-62 (2010). IHC-Fr ; Human . PubMed: 20035048

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    What were the conditions (antibody dilutions and sample types) of the validation tests for the applications listed on the datasheet, including functional assays, IHC of paraffin-embedded tissue, and western blotting? For flow cytometry you specifiy a range of 1/10 - 1/100. What was the sample?

    Read More
    Answer

    The following paper describes production of the clone and characterization in three of the assays we list on the datasheet: Functional Assays, IHC-P, and WB

    http://www.nature.com/icb/journal/v82/n4/full/icb200462a.html

    Leung, E. et al. (2004) Bioassay detects soluble MAdCAM-1 in body fluids.
    Immunol. Cell. Biol. 82: 400-409.

    For flow cytometry testing, the sample was MAdCAM-transfected CHO cells.

    Read More

    Question

    We purchased a Madcam1 antibody ab34143 [17F5] from Abcam a few weeks ago. I’m very sorry to tell you this, but this antibody is not so working well.. It is actually worse than another madcam1 antibody ab34247 [314G8] which we bought last year. With the old madcam1 antibody (314G8), we saw at least low levels of Madcam1 signal on some stromal cells and endothelial cells, but the new madcam1 (ab34143) is just not showing anything at all.

    I checked the Abcam’s website and saw that ab34143 has a reference in the following paper:

    Allavena R et al. CNS elevation of vascular and not mucosal addressin cell adhesion molecules in patients with multiple sclerosis. Am J Pathol 176:556-62 (2010). IHC-Fr; Human.Read more (PubMed: 20035048)

    However, this paper only shows the stainings with ab34247 [314G8], but not with ab34143 [17F5]. Maybe because the performance of ab34143 [17F5] is not as good as the other one? Could you please check with Abcam if 17F5 is validated for IHC-fr and help me to solve this issue?

    Read More
    Answer

    Thank you for contacting us.



    I am sorry to hear you are experiencing difficulties with ab34143 in IHC-Fr. This antibody is garanteed to work on Human samplesin IHC-Fr, Flow Cyt, ELISA, IHC-P andWB.

    We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

    I look forward to receiving your reply.

    Read More

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