Anti-Mad2L2/REV7 抗体 (ab115622)
Key features and details
- Rabbit polyclonal to Mad2L2/REV7
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Mad2L2/REV7 antibody
Mad2L2/REV7 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Mad2L2/REV7 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Horse, Cow, Pig, Xenopus laevis, Zebrafish
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免疫原
Synthetic peptide corresponding to Human Mad2L2/REV7 aa 1-100 conjugated to keyhole limpet haemocyanin.
Database link: NP_006332.2
(Peptide available asab11254) -
ポジティブ･コントロール
- Human Brain, Cerebellum tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab115622の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
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IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation. -
組織特異性
Ubiquitously expressed. -
配列類似性
Contains 1 HORMA domain. -
細胞内局在
Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 10459 Human
- Entrez Gene: 71890 Mouse
- Entrez Gene: 313702 Rat
- Entrez Gene: 394380 Xenopus laevis
- Entrez Gene: 550258 Zebrafish
- Entrez Gene: 557968 Zebrafish
- Omim: 604094 Human
- SwissProt: Q9UI95 Human
see all -
別名
- Homolog of REV7 S cerevisiae antibody
- hREV7 antibody
- MAD2 (mitotic arrest deficient yeast, homolog) like 2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mad2L2/REV7 antibody (ab115622)This image is courtesy of an anonymous Abreview
ab115622 staining Mad2L2/REV7 in Human lung cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with the Hope Technique and blocked for 15 minutes at 25°C. Samples were incubated with primary antibody (1/300) for 1 hour at 25°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mad2L2/REV7 antibody (ab115622)ab115622, at 10µg/ml, staining Mad2L2/REV7 in Formalin-fixed, Paraffin-embedded Human Brain Cerebellum tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab115622 は 1 報の論文で使用されています。
- Karakashev S et al. EZH2 Inhibition Sensitizes CARM1-High, Homologous Recombination Proficient Ovarian Cancers to PARP Inhibition. Cancer Cell 37:157-167.e6 (2020). PubMed: 32004442