Anti-Mad2L1 抗体 (ab70385)
Key features and details
- Rabbit polyclonal to Mad2L1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Mad2L1 antibody
Mad2L1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Mad2L1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
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免疫原
Synthetic peptide mapping to a region between residue 150 and the C-terminus (residue 205) of Human MAD2 (SwissProt Q13257)
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ポジティブ･コントロール
- WB: HeLa, HEK293T, Jurkat, TCMK-1, NIH/3T3; IP: HeLa
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70385の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
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| IP |
Use at 2-10 µg/mg of lysate.
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| 特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa). |
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IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. -
配列類似性
Belongs to the MAD2 family.
Contains 1 HORMA domain. -
ドメイン
The protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. The protein in the closed conformation preferentially dimerizes with another molecule in the open conformation, but can also form a dimer with a molecule in the closed conformation. Formation of a heterotetrameric core complex containing two molecules of MAD1L1 and of MAD2L1 in the closed conformation promotes binding of another molecule of MAD2L1 in the open conformation and the conversion of the open to the closed form, and thereby promotes interaction with CDC20. -
翻訳後修飾
Phosphorylated on multiple serine residues. The level of phosphorylation varies during the cell cycle and is highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20. -
細胞内局在
Nucleus. Chromosome > centromere > kinetochore. Cytoplasm. Recruited by MAD1L1 to unattached kinetochores (Probable). Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD. - Information by UniProt
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参照データベース
- Entrez Gene: 4085 Human
- Entrez Gene: 56150 Mouse
- Omim: 601467 Human
- SwissProt: Q13257 Human
- SwissProt: Q9Z1B5 Mouse
- Unigene: 591697 Human
- Unigene: 485053 Mouse
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別名
- HsMAD 2 antibody
- HsMAD2 antibody
- MAD 2 antibody
see all
画像
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Immunoprecipitation - Anti-Mad2L1 antibody (ab70385)
MAD2 was immunoprecipitated from HeLa whole cell lysate (1 mg per IP reaction, 20% loaded) with ab70385 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab70385 at 1 µg/mL.
Lane 1: ab70385 IP in HeLa whole cell lysate.
Lane 2: ab70385 IP in HeLa whole cell lysate.
Lane 3: rabbit anti-MAD2 antibody IP in HeLa whole cell lysate.
Lane 4: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 minutes
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Western blot - Anti-Mad2L1 antibody (ab70385)All lanes : Anti-Mad2L1 antibody (ab70385) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH/3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 24 kDa
Exposure time: 3 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab70385 は 4 報の論文で使用されています。
- Wang Y et al. Phosphorylation of MAD2 at Ser195 Promotes Spindle Checkpoint Defects and Sensitizes Cancer Cells to Radiotherapy in ATM Deficient Cells. Front Cell Dev Biol 10:817831 (2022). PubMed: 35309941
- Takei Y et al. Expression of p53 synergistically augments caspases-mediated apoptosis induced by replication-competent adenoviruses in pancreatic carcinoma cells. Cancer Gene Ther 22:445-53 (2015). PubMed: 26251031
- Tan CL et al. Stranglehold on the spindle assembly checkpoint: the human papillomavirus E2 protein provokes BUBR1-dependent aneuploidy. Cell Cycle 14:1459-70 (2015). WB, ICC/IF ; Human . PubMed: 25789401
- Jahn SC et al. Constitutive Cdk2 activity promotes aneuploidy while altering the spindle assembly and tetraploidy checkpoints. J Cell Sci 126:1207-17 (2013). PubMed: 23321641